双层胶原支架与人牙周膜细胞的体外生物相容性

发布时间：2018-10-09 07:13

【摘要】：【目的】研究双层胶原支架与人牙周膜细胞的体外生物相容性。【方法】组织块培养法培养原代人牙周膜细胞。MTT法测胶原支架不同浓度浸提液的细胞毒性。将人牙周膜细胞与支架材料三维培养,通过扫描电镜、组织切片观察其复合情况,并检测三维培养后细胞碱性磷酸酶(ALP)、羟脯氨酸(HYP)的分泌情况。【结果】不同浓度材料浸提液毒性评级为0~1级。细胞与支架复合培养后,细胞在支架上黏附、增殖良好,支架材料内部细胞分布均匀,且致密层可起屏障膜作用,阻挡细胞进入支架内部。细胞与胶原支架复合培养24 h后,细胞的ALP活性与对照组无明显差异(P0.05),复合培养48、72 h后,细胞的ALP活性高于对照组(P0.05)。复合培养24、48、72 h后,细胞的HYP活性均高于对照组(P0.05)。【结论】双层胶原支架具有良好的生物相容性,可进一步应用于牙周组织工程的研究。
[Abstract]:[objective] to study the biocompatibility of double collagen scaffolds with human periodontal ligament cells in vitro. The composite of human periodontal ligament cells and scaffold was observed by scanning electron microscope (SEM) and tissue section. The secretion of alkaline phosphatase (ALP),) hydroxyproline (HYP) was detected after three dimensional culture. [results] the toxicity rating of the extracts of different concentrations was 0 ~ 1 grade. After the cells were co-cultured with the scaffolds, the cells adhered to the scaffolds and proliferated well. The cells in the scaffold materials were evenly distributed, and the dense layer could act as a barrier membrane to prevent the cells from entering the scaffold. After co-culture with collagen scaffold for 24 h, the ALP activity of the cells was not significantly different from that of the control group (P0.05), and the ALP activity of the cells was higher than that of the control group (P0.05) after 48 minutes of co-culture (P0.05). The HYP activity of the cells was significantly higher than that of the control group (P0.05). [conclusion] the double-layer collagen scaffold has good biocompatibility and can be further applied to the study of periodontal tissue engineering.
【作者单位】：中山大学附属第三医院口腔科;国家人体组织功能重建工程技术研究中心;
【基金】：广东省科技计划项目(2011B031300011,2010B031100021)
【分类号】：R318.08

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