重力矢量及趋化诱导对脂肪干细胞在PLGA电纺膜上早期增殖的影响
发布时间：2018-10-12 13:48
【摘要】：观察研究重力矢量方向及血小板衍生生长因子(PDGF)趋化诱导,在体外环境对人脂肪干细胞(ASCs)在PLGA电纺膜表面黏附和增殖活动的影响。体外原代培养从人颊脂垫提取的ASCs,根据不同重力矢量条件以及有无趋化因子PDGF将实验分为4个组。A组:PDGF/重力矢量(+),含PDGF的PLGA电纺膜置于ASCs悬液底部;B组:PBS/重力矢量(+),空白(PBS)电纺膜置于ASCs悬液底部;C组:PDGF/重力矢量(-),含PDGF的PLGA电纺膜覆于ASCs悬液表面;D组:PBS/重力矢量(-),空白(PBS)电纺膜覆于ASCs悬液表面。分别于0、1、3、5、8、11d加入Alamar Blue继续孵化24 h后测OD值,对照标准曲线,计算各组Alamar Blue的下降百分比,评估ASCs的黏附增殖活动。结果显示:在重力矢量(+)组的早期(第1~4 d),无论趋化因子PDGF存在与否,Alamar Blue的下降百分比都能达到30%左右,不影响细胞的定植、黏附与增殖。但是在重力矢量(-)组,缺少趋化因子PDGF的Alamar Blue的下降百分比为25%左右,细胞将无法正常的定植与黏附。PDGF具有明显诱导ASCs趋化聚集作用,能减轻不利重力因素的影响,显著改善ASCs黏附与增殖。
[Abstract]:The effects of gravity vector direction and platelet-derived growth factor (PDGF) chemotaxis on the adhesion and proliferation of human adipose stem cell (ASCs) on the surface of PLGA electrospun membrane were studied in vitro. ASCs, extracted from human buccal fat pad in vitro was divided into four groups according to different gravity vector conditions and chemokine PDGF. Group A: PDGF/ gravity vector (), PLGA electrospun film containing PDGF at the bottom of ASCs suspension; group B: PBS/ gravity Vector (), blank (PBS) electrospun film is placed at the bottom of ASCs suspension, C group is PDGF/ gravity vector (-), PLGA electrospun film containing PDGF is coated on ASCs suspension surface, group D is PBS/ gravity vector (-), blank (PBS) electrospun film is coated on ASCs suspension surface. The values of OD were measured after incubating for 24 h after adding Alamar Blue for 24 hours. The percentage of Alamar Blue decreased in each group was calculated and the adhesion and proliferation of ASCs were evaluated. The results showed that in the early stage of gravity vector group (1 ~ 4 d), the percentage of, Alamar Blue decreased by about 30%, regardless of whether the chemokine PDGF existed or not, which did not affect cell colonization, adhesion and proliferation. However, in the gravity vector (-) group, the percentage of Alamar Blue in the absence of chemokine PDGF is about 25%, and the cells will not be able to colonize and adhere normally. PDGF can obviously induce the chemotactic aggregation of ASCs, which can reduce the influence of unfavorable gravity factors. The adhesion and proliferation of ASCs were significantly improved.
【作者单位】： 天津医科大学口腔医院口腔颌面外科;
【基金】：教育部留学回国人员科研启动基金资助项目(教外司留(2012)940)
【分类号】：R318.08
本文编号：2266348
[Abstract]:The effects of gravity vector direction and platelet-derived growth factor (PDGF) chemotaxis on the adhesion and proliferation of human adipose stem cell (ASCs) on the surface of PLGA electrospun membrane were studied in vitro. ASCs, extracted from human buccal fat pad in vitro was divided into four groups according to different gravity vector conditions and chemokine PDGF. Group A: PDGF/ gravity vector (), PLGA electrospun film containing PDGF at the bottom of ASCs suspension; group B: PBS/ gravity Vector (), blank (PBS) electrospun film is placed at the bottom of ASCs suspension, C group is PDGF/ gravity vector (-), PLGA electrospun film containing PDGF is coated on ASCs suspension surface, group D is PBS/ gravity vector (-), blank (PBS) electrospun film is coated on ASCs suspension surface. The values of OD were measured after incubating for 24 h after adding Alamar Blue for 24 hours. The percentage of Alamar Blue decreased in each group was calculated and the adhesion and proliferation of ASCs were evaluated. The results showed that in the early stage of gravity vector group (1 ~ 4 d), the percentage of, Alamar Blue decreased by about 30%, regardless of whether the chemokine PDGF existed or not, which did not affect cell colonization, adhesion and proliferation. However, in the gravity vector (-) group, the percentage of Alamar Blue in the absence of chemokine PDGF is about 25%, and the cells will not be able to colonize and adhere normally. PDGF can obviously induce the chemotactic aggregation of ASCs, which can reduce the influence of unfavorable gravity factors. The adhesion and proliferation of ASCs were significantly improved.
【作者单位】： 天津医科大学口腔医院口腔颌面外科;
【基金】：教育部留学回国人员科研启动基金资助项目(教外司留(2012)940)
【分类号】：R318.08
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