利用原子力显微镜研究壳聚糖膜对细胞弹性的影响
发布时间：2018-10-22 12:41
【摘要】：细胞的弹性性质是细胞力学的一个重要参数，细胞的结构和功能与细胞的力学性质密切相关。外界物理环境的改变，如细胞外基质软硬度的变化会导致细胞弹性发生变化。原子力显微镜可以在生理条件下检测样品表面的力学性质，是研究细胞力学的一个十分有用的工具。 本文以壳聚糖为材料制备了不同软硬的基底膜，利用原子力显微镜研究壳聚糖膜对细胞弹性的影响。通过配制不同浓度的壳聚糖溶液，采用流延法制备了实验所需的壳聚糖膜，并对壳聚糖膜的细胞相容性、接触角、硬度做了测定。将正常的内皮细胞和肝癌细胞分别接种在不同浓度的壳聚糖膜上，对其形态特征（细胞铺展面积、细胞长、短轴比值等）和细胞弹性做了检测。本文的主要结果如下：①壳聚糖膜溶胀性最大的为3%壳聚糖膜，最小的为4%壳聚糖膜；膜的接触角约在65°～81°之间，膜的硬度（复水后）约在315KPa～413KPa之间，膜的接触角和硬度随着制膜溶液浓度的增大而增大；②内皮细胞和肝癌细胞在膜上铺展的面积随着制膜时溶液浓度的增大而增大，细胞长轴与短轴的比率在不同浓度的壳聚糖膜上有所不同，，说明细胞形态在不同的壳聚糖膜上会发生变化；内皮细胞最大的弹性模量约为2.44KPa，最小的约为1.75KPa,最大的弹性模量值是最小模量值的1.4倍，肝癌细胞最大的弹性模量约为1.32KPa，最小的约为0.66KPa，最大的弹性模量值是最小模量值的2倍。这说明不同的壳聚糖膜对细胞的弹性模量有着明显地影响。实验结果有助于了解壳聚糖膜与细胞之间的相互作用，为壳聚糖膜在细胞生物学领域的研究和应用增加理论上的认识。
[Abstract]:The elastic property of cells is an important parameter of cellular mechanics. The structure and function of cells are closely related to the mechanical properties of cells. Changes in physical environment, such as the degree of hardness of extracellular matrix, will lead to changes in cell elasticity. Atomic force microscope (AFM) is a useful tool for studying cellular mechanics because it can detect the mechanical properties of samples under physiological conditions. In this paper, different soft and hard substrate membranes were prepared from chitosan, and the effect of chitosan membrane on cell elasticity was studied by atomic force microscopy (AFM). The chitosan membrane was prepared by different concentration of chitosan solution, and the cell compatibility, contact angle and hardness of chitosan membrane were measured. Normal endothelial cells and hepatoma cells were inoculated on chitosan membrane at different concentrations, and their morphological characteristics (cell spreading area, cell length, short axis ratio, etc.) and cell elasticity were determined. The main results are as follows: (1) the highest swelling property of chitosan membrane is 3% chitosan film, the smallest is 4% chitosan film, the contact angle of the membrane is about 65 掳~ 81 掳, the hardness of the membrane is about 315KPa~413KPa. The contact angle and hardness of the membrane increased with the increase of the concentration of the solution, 2 the area of the endothelial cells and hepatoma cells spreading on the membrane increased with the increase of the concentration of the solution. The ratio of cell long axis to short axis was different in different concentrations of chitosan membrane, which indicated that cell morphology would change on different chitosan membrane. The maximum elastic modulus of endothelial cells is about 2.44 KPA, the smallest is about 1.75 KPA, the maximum elastic modulus is 1.4 times of the minimum, the maximum elastic modulus of hepatoma cells is about 1.32 KPA, the smallest is about 0.66 KPA, and the maximum elastic modulus is 2 times of the minimum. This shows that different chitosan membranes have a significant effect on the elastic modulus of cells. The experimental results are helpful to understand the interaction between chitosan membrane and cells, and to increase the theoretical understanding of the research and application of chitosan membrane in the field of cell biology.
【学位授予单位】：重庆大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.01
本文编号：2287187
[Abstract]:The elastic property of cells is an important parameter of cellular mechanics. The structure and function of cells are closely related to the mechanical properties of cells. Changes in physical environment, such as the degree of hardness of extracellular matrix, will lead to changes in cell elasticity. Atomic force microscope (AFM) is a useful tool for studying cellular mechanics because it can detect the mechanical properties of samples under physiological conditions. In this paper, different soft and hard substrate membranes were prepared from chitosan, and the effect of chitosan membrane on cell elasticity was studied by atomic force microscopy (AFM). The chitosan membrane was prepared by different concentration of chitosan solution, and the cell compatibility, contact angle and hardness of chitosan membrane were measured. Normal endothelial cells and hepatoma cells were inoculated on chitosan membrane at different concentrations, and their morphological characteristics (cell spreading area, cell length, short axis ratio, etc.) and cell elasticity were determined. The main results are as follows: (1) the highest swelling property of chitosan membrane is 3% chitosan film, the smallest is 4% chitosan film, the contact angle of the membrane is about 65 掳~ 81 掳, the hardness of the membrane is about 315KPa~413KPa. The contact angle and hardness of the membrane increased with the increase of the concentration of the solution, 2 the area of the endothelial cells and hepatoma cells spreading on the membrane increased with the increase of the concentration of the solution. The ratio of cell long axis to short axis was different in different concentrations of chitosan membrane, which indicated that cell morphology would change on different chitosan membrane. The maximum elastic modulus of endothelial cells is about 2.44 KPA, the smallest is about 1.75 KPA, the maximum elastic modulus is 1.4 times of the minimum, the maximum elastic modulus of hepatoma cells is about 1.32 KPA, the smallest is about 0.66 KPA, and the maximum elastic modulus is 2 times of the minimum. This shows that different chitosan membranes have a significant effect on the elastic modulus of cells. The experimental results are helpful to understand the interaction between chitosan membrane and cells, and to increase the theoretical understanding of the research and application of chitosan membrane in the field of cell biology.
【学位授予单位】：重庆大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.01
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