人工听骨链重建材料生物相容性、特性及修复喉软骨缺损的效果

发布时间：2018-10-30 07:54

【摘要】：背景:喉软骨缺损替代品较多,包括自体软骨、同种异体软骨以及金属支架等,这些材料在喉软骨缺损中均能取得理想效果,但是也存在许多不足。目的:研究人工听骨链重建材料生物相容性及其特性,探讨人工听骨链重建材料在喉软骨缺损中的修复效果。方法:(1)从新鲜人骨中提取骨形态发生蛋白,将羟基磷灰石高温煅烧后制备多孔羟基磷灰石,将获得的多孔羟基磷灰石听骨放入骨形态发生蛋白溶液中,制备骨形态发生蛋白-羟基磷灰石人工听骨链重建材料,分析材料的生物相容性及特性。(2)将40只新西兰雄性成年白兔随机分为多孔羟基磷灰石组和人工听骨链重建材料组,每组20只。制备新西兰雄性成年白兔喉软骨缺损模型,多孔羟基磷灰石组采用多孔羟基磷灰石材料进行修复,人工听骨链重建材料组采用骨形态发生蛋白-羟基磷灰石人工听骨链重建材料修复。结果和结论:(1)不同孔隙率人工听骨重建材料抗压强度和抗压强度差异有显著性意义;(2)骨形态发生蛋白-羟基磷灰石人工听骨链重建材料外表面空洞未形成对称性球状,外观表现为多边形,空隙尺寸为100-200μm;(3)2组大鼠分别植入骨形态发生蛋白-多孔羟基磷灰石和骨形态发生蛋白-羟基磷灰石人工听骨链重建材料后未见明显的不良反应。但人工听骨链重建材料组出现较多的结缔组织及明显骨性结节,且材料降解速度较快。(4)提示骨形态发生蛋白-羟基磷灰石人工听骨链重建材料具有良好的生物相容性和特性,利用其修复喉软骨缺损效果理想,具有较高的临床应用价值。
[Abstract]:Background: there are many substitutes for laryngeal cartilage defects, including autologous cartilage, allogeneic cartilage and metal scaffolds. These materials can achieve ideal results in laryngeal cartilage defects, but there are many deficiencies. Objective: to study the biocompatibility and characteristics of artificial ossicular chain reconstruction material, and to investigate the effect of artificial ossicular chain reconstruction material in repairing laryngeal cartilage defect. Methods: (1) Bone morphogenetic protein (BMP) was extracted from fresh human bone. Hydroxyapatite was calcined at high temperature to prepare porous hydroxyapatite. The ossicular bone of porous hydroxyapatite was put into the solution of bone morphogenetic protein (BMP). Bone morphogenetic protein-hydroxyapatite artificial ossicular chain reconstruction materials were prepared. (2) 40 New Zealand male adult rabbits were randomly divided into porous hydroxyapatite group and artificial ossicular chain reconstruction group with 20 rabbits in each group. The laryngeal cartilage defect model of New Zealand adult white rabbits was established. The porous hydroxyapatite group was repaired with porous hydroxyapatite material. Bone morphogenetic protein-hydroxyapatite artificial ossicular chain reconstruction material was used in the artificial ossicular chain reconstruction group. Results and conclusions: (1) there were significant differences in compressive strength and compressive strength of artificial ossicular reconstruction materials with different porosity; (2) the outer surface cavity of bone morphogenetic protein-hydroxyapatite artificial ossicular chain was not symmetrical globular, and the appearance was polygon, the void size was 100-200 渭 m; (3) there was no significant adverse reaction after the implantation of bone morphogenetic protein-porous hydroxyapatite and bone morphogenetic protein-hydroxyapatite artificial ossicular chain reconstruction. But there were more connective tissues and obvious bony nodules in the artificial ossicular chain reconstruction group. The results showed that bone morphogenetic protein-hydroxyapatite artificial ossicular chain reconstruction material had good biocompatibility and characteristics. It has high clinical application value.
【作者单位】：华中科技大学同济医学院附属武汉市中心医院;
【基金】：2013年河北省自然科学基金项目(2013CFC082)~~
【分类号】：R318.08;R767.91

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