激光和LED对酶反应生产神经酰胺的影响

发布时间：2018-10-30 10:53

【摘要】：激光照射对酶活性的影响主要取决于光源的波长和能量。系统研究了激光的波长和能量对酶活性的改变,并且对激光和发光二极管(LED)是否对酶活性改变具有相同的效果也进行了研究。分别采用能量为0~810J/cm2,激光波长分别为532、808、1064、1342nm,LED波长分别为640nm和810nm的激光照射磷脂酶C,通过高效薄层层析法(HPTLC)测量经过光源照射0.5、1、2、3、4、6、17、24h后神经酰胺的浓度来研究酶活性的改变。酶活性改变持续时间通过以上实验数据进行评估计算。在一定波长范围内激光和LED照射均可以提高磷脂酶C的活性,效果取决于光源的能量和波长,在经过照射后,酶活性的改变大约可以持续4h。研究结果表明在报道激光照射对酶活性改变的时候,酶活性改变持续时间应该作为一个主要的激光参数进行声明,还发现照射时选择波长、能量相同的激光源和LED,其对酶活性的改变具有同样的效果。
[Abstract]:The effect of laser irradiation on enzyme activity mainly depends on the wavelength and energy of light source. The effects of laser wavelength and energy on enzyme activity were systematically studied, and the effect of laser and light-emitting diode (LED) on enzyme activity was also studied. Phospholipase C was irradiated by laser irradiation with energy of 0 ~ 810J / cm ~ (2) and laser wavelength of 532n / 808 / 1064nmLED with 640nm and 810nm, respectively, and measured by high performance thin-layer chromatography (HPTLC). The concentration of ceramide was used to study the change of enzyme activity after 24 h. The duration of enzyme activity change was evaluated by the above experimental data. The activity of phospholipase C can be increased by laser and LED irradiation in a certain wavelength range. The effect depends on the energy and wavelength of light source. After irradiation, the change of enzyme activity can last about 4 hours. The results show that the duration of enzyme activity change should be declared as a main laser parameter when the laser irradiation changes the enzyme activity. It is also found that the laser source and LED, with the same wavelength and same energy are selected during the irradiation. It has the same effect on the change of enzyme activity.
【作者单位】：山西省肿瘤医院;中国医学科学院北京协和医学院生物医学工程研究所激光医学实验室;
【分类号】：TN249;R318.51

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