聚氨酯—硫酸软骨素膜的制备及其抗结壳性能研究

发布时间：2018-11-28 21:15

【摘要】：硬性结壳并诱发感染是影响输尿管支架的临床治疗效果和寿命的根本原因。而支架植入体内后快速形成的条件膜是引起结壳的关键因素之一。聚氨酯(PU)是输尿管支架常用材料之一,具有良好的生物相容性和优异的物理机械性能,而其固有的疏水性特征利于蛋白吸附和细菌粘附生长,从而导致支架结壳和引发炎症问题。聚氨酯的表面改性是解决支架存在问题的良好策略之一。本文商业化的PU Pellethane 2363-80AE为基底,利用化学接枝手段在聚氨酯表面引入具有亲水性和带负电荷的硫酸软骨素,通过加入不同的间隔基以及间隔基的投入量得到表面性能不同的聚氨酯-硫酸软骨素(PU-CS)膜片。研究特殊表面结构和物理化学性能对蛋白、粘多糖等生物大分子的吸附情况,以及表面形成条件膜后,对无机盐沉积、细菌粘附和生长的影响。结果显示,改性后的PU-CS膜片亲水性更好,且表现出良好的蛋白抑制效果,其中丙二胺为间隔基,且CS接枝量最多的PU-CS(3)对蛋白吸附有最佳的抵抗效果。改性后膜片表面条件膜的形成受到了抑制,蛋白和粘多糖的吸附量减少；条件膜中的蛋白可以促进无机盐的沉积,粘多糖则可以通过减小无机盐的粒径而减少其沉积,第7天PU-CS(3)膜片上的无机盐沉积最少；CS改性后的PU膜片表现出更少的细菌粘附数量,条件膜中蛋白的减少和粘多糖含量的增加,都会有利于减少细菌粘附,且PU-CS(3)膜片上细菌粘附最少。研究结果可为支架表面改性提供实验依据,设计具有抗蛋白粘附和利于粘多糖粘附的表面,将可能提高支架的抗结壳性能。
[Abstract]:Rigid crusts and induced infections are the fundamental factors affecting the clinical efficacy and life span of ureteral stents. The rapid formation of conditional membrane after stent implantation is one of the key factors for the formation of crusts. Polyurethane (PU) is one of the common materials for ureteral stents. It has good biocompatibility and excellent physical and mechanical properties, and its inherent hydrophobic properties are conducive to protein adsorption and bacterial adhesion growth. This leads to scaffold crusts and inflammatory problems. The surface modification of polyurethane is one of the good strategies to solve the problems of the scaffold. In this paper, commercial PU Pellethane 2363-80AE was used as substrate to introduce chondroitin sulfate with hydrophilic and negative charge onto polyurethane surface by chemical grafting. The polyurethane-chondroitin sulfate (PU-CS) membrane with different surface properties was obtained by adding different spacers and spacer inputs. The adsorption of protein, mucopolysaccharide and other biomolecules by special surface structure and physicochemical properties, and the effect of the formation of conditioned film on inorganic salt deposition, bacterial adhesion and growth were studied. The results showed that the modified PU-CS film was more hydrophilic and had a good protein inhibition effect. PU-CS (3), which was the spacer group of propylenediamine and the grafted CS, had the best resistance to protein adsorption. The formation of membrane on the surface of modified membrane was inhibited, and the adsorption of protein and mucopolysaccharide was decreased. The protein in the conditioned film could promote the deposition of inorganic salt, while mucopolysaccharide could reduce the deposition of inorganic salt by reducing the particle size of inorganic salt, and on the 7th day, the deposition of inorganic salt on PU-CS (3) membrane was the least. The CS modified PU membrane showed less bacterial adhesion. The decrease of protein and the increase of mucopolysaccharide content in the conditioned membrane would help to reduce the bacterial adhesion, and the bacterial adhesion on PU-CS (3) membrane was the least. The results can provide experimental basis for surface modification of scaffolds and design surfaces with anti-protein adhesion and favorable mucopolysaccharide adhesion, which may improve the anti-crusting performance of scaffolds.
【学位授予单位】：华东理工大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R318.08

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