丝素蛋白生物医学材料的制备及模拟生理条件下的药物释放研究
发布时间：2018-12-07 13:18
【摘要】：随着对丝素蛋白结构和功能的不断深入研究,丝素的应用发生了巨大的变化,不再单一地局限于编织,其开发应用范围已拓宽到了非纺织领域,如医药化工、保健品、环境探测等方面。同时,丝素蛋白具有良好的机械性、对生物体无毒且透气又透氧。因此,在组织工程研究方面表现出不可估量的开发潜力。 论文首先制备丝素蛋白膜通过溶液浇注而成,应用傅里叶红外光谱(FTIR)和X-射线衍射(XRD)分析了经浓度不同的甲、乙醇-水混合溶剂处理后的丝素蛋白薄膜的结构变化,并以罗丹明B为模型药物与丝素蛋白建立药物缓释系统,研究了丝素蛋白膜的结晶结构对药物释放动力学的影响情况。实验发现,在醇体积比浓度φMeOH/%=50～90的范围内,丝素蛋白材料中以p-折叠为主的silk Ⅱ结晶含量随着混合溶剂中醇浓度的增加而先增加后下降。甲醇-水混合溶剂中,在φMeOH/%=80附近出现最大值。而在乙醇-水混合溶剂中,在70-80%附近出现最大值。应用Peppas方程分析了罗丹明B从丝素蛋白膜的释放动力学,结果表明,罗丹明B从丝素蛋白膜的释放属于Fickian扩散机理,其扩散指数n随着丝素蛋白膜中p-折叠含量的增加而增加。silk Ⅱ结晶是丝素蛋白材料药物释放的天然调节器。 在此基础上选用不同比例的丙三醇与丝素蛋白共混成膜,应用傅里叶红外光谱(FTIR)和X-射线衍射(XRD)研究了丝素蛋白-丙三醇共混膜的结晶结构的变化,并以罗丹明B为模型药物与丝素蛋白建立药物缓释系统,研究了丝素蛋白膜的结晶结构对药物释放动力学的影响情况。结果显示,在未经甲醇-水混合溶剂处理的丝素蛋白-丙三醇共混膜中,随着丙三醇含量的提高,丝素蛋白材料中以p-折叠为主的silk Ⅱ结晶结构含量逐渐增大,在丙三醇含量为30%时出现最大值。罗丹明B从共混药物膜的释放属于Fickian扩散机理,其扩散指数n随着丝素蛋白膜中β-折叠含量的增加而增加,且药物释放百分数增大,丙三醇的存在可以改善丝素蛋白为载体的药物释放效果。
[Abstract]:With the in-depth study of the structure and function of silk fibroin, the application of silk fibroin has changed greatly, and it is no longer confined to weaving, and its development and application has been extended to non-textile fields, such as medicine, chemical industry, health products, etc. Environmental detection etc. At the same time, silk fibroin has good mechanical properties, non-toxic to organism, breathable and oxygen permeable. Therefore, there is inestimable development potential in tissue engineering research. Firstly, silk fibroin films were prepared by solution pouring. The structural changes of silk fibroin films treated with different concentrations of methyl-ethanol-water solution were analyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The drug release system was established with Rhodamine B as model drug and silk fibroin. The effect of crystalline structure of silk fibroin membrane on drug release kinetics was studied. It was found that in the range of alcohol volume ratio 蠁 MeOH/%=50~90, the content of silk 鈪，
本文编号：2367265
[Abstract]:With the in-depth study of the structure and function of silk fibroin, the application of silk fibroin has changed greatly, and it is no longer confined to weaving, and its development and application has been extended to non-textile fields, such as medicine, chemical industry, health products, etc. Environmental detection etc. At the same time, silk fibroin has good mechanical properties, non-toxic to organism, breathable and oxygen permeable. Therefore, there is inestimable development potential in tissue engineering research. Firstly, silk fibroin films were prepared by solution pouring. The structural changes of silk fibroin films treated with different concentrations of methyl-ethanol-water solution were analyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The drug release system was established with Rhodamine B as model drug and silk fibroin. The effect of crystalline structure of silk fibroin membrane on drug release kinetics was studied. It was found that in the range of alcohol volume ratio 蠁 MeOH/%=50~90, the content of silk 鈪，
本文编号：2367265
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