接枝不同官能团的聚酯材料对血小板黏附及其功能的影响研究
发布时间：2018-12-15 03:46
【摘要】：目的考察接枝不同官能团(-COOH、-OH、两性基团)的聚对苯二甲酸丁二醇酯(PBT)膜对血小板黏附及血小板功能的影响,选择适用于过滤去除浓缩血小板中白细胞的材料。方法利用紫外改性的方法,将不同单体包括丙烯酸(AA)、丙烯酸羟乙酯(HEA)和N-(3-磺丙基)-N-甲基丙烯酸基氧乙基-N,N-二甲基铵内铵盐(SMDB)接枝到PBT表面,得到接枝不同官能团的PBT膜,包括PBT-AA(-COOH)、PBT-HEA(-OH)、PBT-SMDB(两性基团);通过傅里叶红外光谱、水接触角及表面粗糙度等指标对改性PBT的理化性能做表征,采用扫描电镜观察改性PBT对血小板的黏附,并通过检测PBT改性前后的血小板聚集率、低渗休克相对变化、CD62p表达率等指标,考察改性PBT对血小板功能的影响。结果在红外光谱的1 047和1 043 cm-1处出现了C-O-C的弯曲振动峰和O=S=O的对称伸缩振动峰,提示HEA和SMDB接枝到了PBT膜表面。扫描电镜显示:未改性的PBT膜表面黏附的血小板数明显多于改性PBT膜表面,且可以观察到明显的伪足。血小板黏附数在接枝不同官能团的PBT膜表面顺序为:-COOH两性基团-OH。未改性PBT组(n=6)与对照组(未接触材料的血小板)(n=6)比较:血小板最大聚集率(%)为87.22±2.10 vs 82.36±2.11,低渗休克相对变化率(%)为79.33±1.27 vs 77.12±1.09,CD62p表达率增加(%)为9.78±0.58 vs 17.45±1.25(P0.05)。在接枝不同官能团的改性PBT膜中,PBT-HEA(n=6)对血小板聚集和血小板活化等功能影响最小[血小板最大聚集率(93.34±1.45)%、血小板低渗休克相对变化率(81.26±1.48)%、CD62p表达率(12.51±0.85)%]。结论表面接枝HEA的聚酯对血小板的黏附及功能影响明显小于接枝其他官能团的材料,或可作为用于浓缩血小板过滤去除白细胞的新材料。
[Abstract]:Objective to investigate the effects of poly (butylene terephthalate) (PBT) membranes grafted with different functional groups (- COOH,-OH,) on platelet adhesion and platelet function. Methods different monomers, including (AA), hydroxyethyl acrylate (HEA) and N- (3-sulfopropyl) -N-methacrylate ethoxyethyl N, were modified by UV. (SMDB) was grafted onto the surface of PBT to obtain PBT membrane grafted with different functional groups, including PBT-AA (- COOH), PBT-HEA (- OH), PBT-SMDB). The physicochemical properties of modified PBT were characterized by FTIR, water contact angle and surface roughness. The adhesion of modified PBT to platelet was observed by scanning electron microscope, and the platelet aggregation rate before and after PBT modification was measured. The effects of modified PBT on platelet function were investigated by relative changes of hypotonic shock and CD62p expression. Results the bending vibration peak of C-O-C and the symmetrical stretching vibration peak of O=S=O appeared at 1 047 and 1 043 cm-1 of IR spectrum, suggesting that HEA and SMDB grafted onto the surface of PBT film. Scanning electron microscopy showed that the number of platelets adhered to the surface of the unmodified PBT membrane was significantly higher than that of the modified PBT membrane, and obvious pseudopodia could be observed. The order of platelet adhesion number on the surface of PBT membrane grafted with different functional groups is-COOH amphoteric group-OH. The maximum platelet aggregation rate (%) was 87.22 卤2.10 vs (82.36 卤2.11) in the unmodified PBT group and the control group (non-contact material platelets). The relative change rate of hypotonic shock (%) was 79.33 卤1.27 vs, 77.12 卤1.09 vs, the expression rate of CD62p increased (%) was 9.78 卤0.58 vs 17.45 卤1.25 (P0.05). Among the modified PBT membranes grafted with different functional groups, PBT-HEA (NN6) had the least effect on platelet aggregation and platelet activation [the maximum platelet aggregation rate was (93.34 卤1.45)%]. The relative change rate of platelet hypotonic shock was (81.26 卤1.48)% and the expression rate of CD62p was (12.51 卤0.85)%. Conclusion the effect of polyester grafted with HEA on platelet adhesion and function is obviously smaller than that on other functional groups, and it may be used as a new material for platelet filtration and leukocyte removal.
【作者单位】： 中国医学科学院北京协和医学院输血研究所;
【基金】：国家自然科学青年科学基金项目51403238)
【分类号】：R318.08;TQ317
，
本文编号：2379908
[Abstract]:Objective to investigate the effects of poly (butylene terephthalate) (PBT) membranes grafted with different functional groups (- COOH,-OH,) on platelet adhesion and platelet function. Methods different monomers, including (AA), hydroxyethyl acrylate (HEA) and N- (3-sulfopropyl) -N-methacrylate ethoxyethyl N, were modified by UV. (SMDB) was grafted onto the surface of PBT to obtain PBT membrane grafted with different functional groups, including PBT-AA (- COOH), PBT-HEA (- OH), PBT-SMDB). The physicochemical properties of modified PBT were characterized by FTIR, water contact angle and surface roughness. The adhesion of modified PBT to platelet was observed by scanning electron microscope, and the platelet aggregation rate before and after PBT modification was measured. The effects of modified PBT on platelet function were investigated by relative changes of hypotonic shock and CD62p expression. Results the bending vibration peak of C-O-C and the symmetrical stretching vibration peak of O=S=O appeared at 1 047 and 1 043 cm-1 of IR spectrum, suggesting that HEA and SMDB grafted onto the surface of PBT film. Scanning electron microscopy showed that the number of platelets adhered to the surface of the unmodified PBT membrane was significantly higher than that of the modified PBT membrane, and obvious pseudopodia could be observed. The order of platelet adhesion number on the surface of PBT membrane grafted with different functional groups is-COOH amphoteric group-OH. The maximum platelet aggregation rate (%) was 87.22 卤2.10 vs (82.36 卤2.11) in the unmodified PBT group and the control group (non-contact material platelets). The relative change rate of hypotonic shock (%) was 79.33 卤1.27 vs, 77.12 卤1.09 vs, the expression rate of CD62p increased (%) was 9.78 卤0.58 vs 17.45 卤1.25 (P0.05). Among the modified PBT membranes grafted with different functional groups, PBT-HEA (NN6) had the least effect on platelet aggregation and platelet activation [the maximum platelet aggregation rate was (93.34 卤1.45)%]. The relative change rate of platelet hypotonic shock was (81.26 卤1.48)% and the expression rate of CD62p was (12.51 卤0.85)%. Conclusion the effect of polyester grafted with HEA on platelet adhesion and function is obviously smaller than that on other functional groups, and it may be used as a new material for platelet filtration and leukocyte removal.
【作者单位】： 中国医学科学院北京协和医学院输血研究所;
【基金】：国家自然科学青年科学基金项目51403238)
【分类号】：R318.08;TQ317
，
本文编号：2379908
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