基于反离子电渗透原理的无创血糖检测研究

发布时间：2018-12-15 04:29

【摘要】：糖尿病是影响人类健康的三大杀手之一，世界上糖尿病患者数量巨大且越来越多，目前没有根治糖尿病的方法，只有通过频繁的血糖浓度检测，注射胰岛素控制血糖浓度来预防和治疗糖尿病及其并发症。无创血糖检测技术是血糖检测研究的热点，主要研究方法是近红外光法和反离子电渗透法。本文探讨了利用反离子电渗透原理进行组织液葡萄糖提取和渗透出的组织液葡萄糖浓度测定研究，设计了能够用于葡萄糖检测的电流型葡萄糖传感器，并利用单片机集成电路技术，以STC89C51RC为核心，设计能够进行初步提取和检测葡萄糖浓度的血糖检测装置。论文主要完成的工作如下：（1）完成了基于丝网印刷工艺的三电极电流型葡萄糖传感器研制，经CHI600A电化学工作站检测，该传感器具有较好的氧化还原性、线性度、可重复性及一致性。在葡萄糖溶液浓度为4~22mmol/L时，传感器的电流响应与葡萄糖浓度间有较好的线性关系，同一批次和不同批次设计的传感器测量的平均偏差为0.85%，具有较好的重复性和一致性。本传感器初步实现了mmol/L级葡萄糖浓度的检测。（2）研制了基于反离子电渗透原理的血糖检测组织液提取和检测装置。以STC89C51RC主控芯片为基础，研制了0.1mA/cm2的恒流源提取电路和5.0~11.0V可调的恒压源电路，用于葡萄糖的提取实验。还研制了弱电流信号的检测和显示电路用于葡萄糖溶液的显示。经电路调试发现，设计的恒压源和恒流源电路达到所需电流和电压要求，可用于葡萄糖的提取；实现了信号采集和显示功能，，能用于葡萄糖传感器上产生弱电流信号的检测和显示。（3）最后，利用该装置对4~22mmol/L的葡萄糖溶液进行测试，验证所研制的传感器和无创血糖检测装置的性能。试验结果表明，装置能把不同浓度的葡萄糖溶液很好的区分，且检测结果与三诺安稳血糖仪检测结果的相关系数为0.9980。
[Abstract]:Diabetes is one of the three major killers affecting human health. There are large and growing numbers of diabetes patients in the world. There is no cure for diabetes at present, only through frequent blood glucose testing. Insulin injection controls blood glucose levels to prevent and treat diabetes and its complications. Non-invasive blood glucose detection is a hot spot in blood glucose detection. The main research methods are near infrared light and reverse ion electroosmosis. In this paper, using the principle of reverse ion electroosmosis, we have studied the determination of glucose concentration in tissue solution for glucose extraction and osmosis, and designed an amperometric glucose sensor which can be used for glucose detection. Using the integrated circuit technology of single chip computer and STC89C51RC as the core, a blood glucose detection device was designed to carry out preliminary extraction and detection of glucose concentration. The main work of this thesis is as follows: (1) the three-electrode current type glucose sensor based on screen printing technology has been developed and tested by CHI600A electrochemical workstation. The sensor has good redox and linearity. Repeatability and consistency. When the concentration of glucose solution is 4~22mmol/L, there is a good linear relationship between the current response of the sensor and the concentration of glucose. The average deviation of the sensor designed for the same batch and different batches is 0.85. It has good repeatability and consistency. This sensor has preliminarily realized the detection of mmol/L grade glucose concentration. (2) based on the principle of reverse ion electroosmosis, a device for extracting and detecting tissue fluid for blood glucose detection was developed. Based on the STC89C51RC main control chip, a constant current source extraction circuit for 0.1mA/cm2 and an adjustable constant voltage source circuit of 5.0 ~ 11.0V have been developed for glucose extraction experiments. A weak current signal detection and display circuit is also developed for glucose solution display. The circuit debugging shows that the designed constant voltage source and constant current source circuit meet the required current and voltage requirements and can be used to extract glucose. The function of signal acquisition and display is realized, which can be used to detect and display weak current signal generated on glucose sensor. (3) finally, the glucose solution of 4~22mmol/L was tested by the device to verify the performance of the sensor and the non-invasive blood glucose detection device. The experimental results show that the device can distinguish different concentrations of glucose solution well, and the correlation coefficient between the detection results and the results of Sannoan stable blood glucose meter is 0.9980.
【学位授予单位】：重庆理工大学
【学位级别】：硕士
【学位授予年份】：2013
【分类号】：R318.6

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