基于DSP的实时PCR仪温度控制系统的研制
发布时间：2019-01-01 20:54
【摘要】：1983年，聚合酶链式反应（Polymerase Chain Reaction，PCR）由Kary B．Mullis博士发明。PCR技术是一项革命性的创新，它在生物医学领域具有重要的作用。实时PCR通过在PCR反应体系中加入荧光探针，利用荧光信号累积实时监测整个PCR进程，最后通过标准曲线对未知模板进行分析。由于此项技术灵敏度高，准确性强，因此已广泛应用于生物医学领域，如医学诊断、食品安全、动物检疫和遗传学研究等。 本文旨在开发一套基于DSP的实时PCR仪温度控制系统，论文工作主要包括硬件开发和系统底层程序开发两个部分。 硬件主要包括：DSP主控电路、驱动电路、半导体加热制冷片、Kapton加热板以及温度传感电路。DSP主控电路则包括电源电路、时钟电路、JTAG接口、复位电路、PWM波输出管脚、A/D转换电路和串行通信接口；驱动电路由光电隔离电路和H桥功率放大电路构建而成；采用半导体加热制冷片和Kapton加热板分别对样品池和热盖进行温度控制；采用铂热电阻Pt100作为温度传感器，并设计了消除非线性误差的电桥电路进行温度探测。 系统底层程序主要包括：软件初始化子程、A/D转换子程、PID算法子程、PWM波输出子程、串口通信子程和Flash程序的烧写。 最后，对所研制的温度控制系统进行了调试。实验结果表明，，A/D转换的相对误差为0.16%；样品池的升降温速率为4℃/s，温度控制精度为±0.25℃；Kapton加热板的升温速率为2.2℃/s，温度控制精度为±0.3℃；电泳结果表明本系统满足PCR反应条件；温度数据可通过串行通信接口以9600的波特率传输至上位机，并由上位机软件绘制温度曲线。所开发的温度控制系统已经历长时间的持续测试，系统运行稳定。
[Abstract]:In 1983, polymerase chain reaction (Polymerase Chain Reaction,PCR) was invented by Dr. Kary B.Mullis. PCR technology is a revolutionary innovation and plays an important role in biomedicine. By adding fluorescence probe into PCR reaction system, real-time PCR monitors the whole PCR process by fluorescence signal accumulation. Finally, the unknown template is analyzed by standard curve. Due to its high sensitivity and accuracy, it has been widely used in biomedical fields, such as medical diagnosis, food safety, animal quarantine and genetic research. The purpose of this paper is to develop a real time PCR temperature control system based on DSP. This paper mainly includes two parts: hardware development and system bottom program development. The hardware includes: DSP main control circuit, driving circuit, semiconductor heating chiller, Kapton heating board and temperature sensing circuit. DSP main control circuit includes power supply circuit, clock circuit, JTAG interface, reset circuit, PWM wave output pin, Ar / D conversion circuit and serial communication interface; The driving circuit is composed of optoelectronic isolation circuit and H-bridge power amplifier circuit, and the temperature of the sample cell and the hot cover are controlled by using the semiconductor heating chiller and the Kapton heating plate, respectively. The platinum thermal resistance (Pt100) is used as the temperature sensor, and a bridge circuit is designed to eliminate the nonlinear error to detect the temperature. The bottom program of the system mainly includes: software initialization sub-program, A / D conversion sub-procedure, PID algorithm sub-path, PWM wave output sub-path, serial communication sub-program and Flash program burning. Finally, the temperature control system is debugged. The experimental results show that the relative error of A- / D conversion is 0.16, the temperature control precision is 卤0.25 鈩
本文编号：2398097
[Abstract]:In 1983, polymerase chain reaction (Polymerase Chain Reaction,PCR) was invented by Dr. Kary B.Mullis. PCR technology is a revolutionary innovation and plays an important role in biomedicine. By adding fluorescence probe into PCR reaction system, real-time PCR monitors the whole PCR process by fluorescence signal accumulation. Finally, the unknown template is analyzed by standard curve. Due to its high sensitivity and accuracy, it has been widely used in biomedical fields, such as medical diagnosis, food safety, animal quarantine and genetic research. The purpose of this paper is to develop a real time PCR temperature control system based on DSP. This paper mainly includes two parts: hardware development and system bottom program development. The hardware includes: DSP main control circuit, driving circuit, semiconductor heating chiller, Kapton heating board and temperature sensing circuit. DSP main control circuit includes power supply circuit, clock circuit, JTAG interface, reset circuit, PWM wave output pin, Ar / D conversion circuit and serial communication interface; The driving circuit is composed of optoelectronic isolation circuit and H-bridge power amplifier circuit, and the temperature of the sample cell and the hot cover are controlled by using the semiconductor heating chiller and the Kapton heating plate, respectively. The platinum thermal resistance (Pt100) is used as the temperature sensor, and a bridge circuit is designed to eliminate the nonlinear error to detect the temperature. The bottom program of the system mainly includes: software initialization sub-program, A / D conversion sub-procedure, PID algorithm sub-path, PWM wave output sub-path, serial communication sub-program and Flash program burning. Finally, the temperature control system is debugged. The experimental results show that the relative error of A- / D conversion is 0.16, the temperature control precision is 卤0.25 鈩
本文编号：2398097
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