同步辐射显微断层成像监测口腔骨植入材料置入体内后的生物相容性及定量分析:随机对照动物实验方案

发布时间：2019-01-17 06:48

【摘要】：背景:目前骨缺损修复的主要方式是在缺损区充填骨替代材料,为了解骨缺损修复效果,需深入了解骨替代材料植入后骨缺损微细结构改变。同步辐射显微断层成像技术(SR-μCT)可以在不用切片、染色的情况下,进行高衬度分辨率、高空间分辨率的显微三维成像,有极高的科研及临床应用价值。目的:探讨在口腔医学检查中应用SR-μCT对骨结合微米级检查的可行性。方法:实验为随机对照动物实验,在中国天津,天津市第五中心医院完成。取24只雄性新西兰大白兔制备下颌骨缺损模型,随机分为4组,分别在缺损区植入自体骨、Bio-oss骨粉、β-磷酸三钙骨粉,阴性对照组不植入任何材料。术后2,4,8周取材(包括缺损区及周围部分正常骨组织),行SR-μCT检查,之后制成组织切片行组织病理学检查,从多角度观察不同种类骨移植材料的骨修复情况。实验方案经天津市第五中心医院伦理委员会批准。新西兰大白兔的实验操作和取材遵循《关于善待实验动物的指导性意见》规定,并与美国国立卫生与健康研究院的指南一致。结果与结论:实验证实了SR-μCT能在不破坏样品的情况下,完成对骨结合及新生骨微观的观察,得到骨体积、骨小梁数目及骨密度等的更准确的定量数据。因此,通过SR-μCT能全面分析骨植入材料置入体内后的生物相容性反应,深入了解不同种类骨移植材料在骨缺损处微米级结构改变,可为提高骨缺损愈合的效果提供实验依据。
[Abstract]:Background: at present, the main method of bone defect repair is to fill the defect area with bone substitute material. In order to understand the effect of bone defect repair, it is necessary to deeply understand the microstructural changes of bone defect after bone replacement material implantation. Synchrotron radiation microtomography (SR- 渭 CT) is of great value in scientific research and clinical application because of its high contrast resolution and high spatial resolution. Objective: to investigate the feasibility of applying SR- 渭 CT to bone bonding micron examination in stomatology. Methods: the experiment was conducted in Tianjin, China, Tianjin Fifth Central Hospital. Twenty-four male New Zealand rabbits were randomly divided into 4 groups. Autogenous bone, Bio-oss bone powder, 尾 -tricalcium phosphate bone powder were implanted in the defect area, but no material was implanted in the negative control group. SR- 渭 CT was performed 8 weeks after operation, and then histopathological examination was performed to observe the bone repair of different kinds of bone graft materials from various angles. The experimental scheme was approved by the Ethics Committee of Tianjin Fifth Center Hospital. The experimental operation and sampling of New Zealand white rabbits follow the guidelines on the treatment of Experimental Animals and are consistent with the guidelines of the National Institutes of Health and Health of the United States. Results & conclusion: SR- 渭 CT was proved to be able to obtain more accurate quantitative data of bone volume, trabecular number and bone density without destroying the samples. Therefore, SR- 渭 CT can be used to analyze the biocompatibility of bone implant materials after implantation, and to understand the micron structure changes of different kinds of bone graft materials at the bone defect, which can provide experimental basis for improving the healing effect of bone defect.
【作者单位】：天津市第五中心医院口腔科;
【基金】：天津市滨海新区卫生计生委科技项目重点支持项目(2015BWKZ001)~~
【分类号】：R783.1

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