聚乙烯醇—透明质酸—胶原组织工程支架的制备和表征

发布时间：2019-02-14 23:54

【摘要】：目的：本研究制备了合成高分子材料聚乙烯醇与天然高分子材料透明质酸复合材料，经过胶原修饰后，制备了聚乙烯醇-透明质酸-胶原复合支架材料，并探讨其作为组织工程支架材料的可行性。方法：采用成膜法和冷冻干燥法，在不同重均分子质量和醇解度的聚乙烯醇中加入透明质酸溶液，逐步增加透明质酸的含量，得到聚乙烯醇-透明质酸复合材料。对材料进行孔隙率、膨胀率、含水率的测试，并用扫描电镜观察材料内部形态结构，选出具有最佳性能的聚乙烯醇-透明质酸复合支架。在最佳配比的聚乙烯醇-透明质酸基础上加入胶原组分，改变材料中胶原的含量，制备出多种浓度配比的聚乙烯醇-透明质酸-胶原支架。材料用EDC交联，对支架进行孔隙率、膨胀率、含水率测试，观察交联前后材料性能变化，扫描电镜观察材料组织形态结构，以及进行材料相容性测试。结果：聚乙烯醇-透明质酸复合膜支架材料含水率在50%-70%范围内,膨胀率在144%-222%之间,孔隙率在60-70%范围内波动。对于冻干海绵材料,支架含水率为64%-75%。扫面电镜观察可见材料内部的孔洞结构。选择最佳配比浓度为5%、分子量为24000的PVA,透明质酸浓度为5%，加入胶原溶液,调整溶液中胶原与透明质酸比例,形成三种组份的复合支架，并使用50mM EDC交联对比。所得材料含水率在66%-70%之间，孔隙率大于在60%-75%之间，，膨胀率达220%-260%。扫描电镜图像结果显示材料内部具有丰富的空洞结构。细胞相容性实验证明材料无毒。结论：制得聚乙烯醇-透明质酸-胶原复合材料有丰富的孔隙结构及良好的细胞相容性特征，满足组织工程支架材料的要求，可用作组织工程支架材料。
[Abstract]:Objective: to prepare the composite of polyvinyl alcohol (PVA) and hyaluronic acid (HA), and to prepare the composite scaffold of polyvinyl alcohol (PVA), hyaluronic acid (HA) and collagen after collagen modification. Its feasibility as scaffold material for tissue engineering was discussed. Methods: polyvinyl alcohol-hyaluronic acid composites were obtained by adding hyaluronic acid solution into polyvinyl alcohol with different weight average molecular weight and alcoholysis degree by means of film forming and freeze-drying, and gradually increasing the content of hyaluronic acid. The porosity, expansion rate and moisture content of the material were measured, and the morphology of the composite scaffold was observed by scanning electron microscope. The composite scaffold with the best performance was selected as the polyvinyl alcohol-hyaluronic acid composite scaffold. Polyvinyl alcohol-hyaluronic acid-collagen scaffold with different concentrations was prepared by adding collagen component on the basis of the optimum proportion of PVA-HA and changing the content of collagen in the material. EDC crosslinking was used to test the porosity, expansion rate and moisture content of the scaffolds. The properties of the scaffolds were observed before and after crosslinking, and the microstructure and compatibility of the scaffolds were observed by SEM. Results: the moisture content of polyvinyl alcohol-hyaluronic acid composite membrane scaffold was between 50% and 70%, the expansion ratio was between 144% and 222%, and the porosity fluctuated in the range of 60% to 70%. For freeze-dried sponge, the moisture content of the support is 64-75. The pore structure in the material was observed by SEM. The optimum concentration of PVA, hyaluronic acid with molecular weight of 24000 was 5 and the optimum ratio of collagen to hyaluronic acid was 5. The ratio of collagen to hyaluronic acid in the solution was adjusted to form a composite scaffold with three components. 50mM EDC was used to crosslink the scaffolds. The moisture content of the obtained material is between 66% and 70%, the porosity is greater than 60%-75%, and the expansion ratio is 220-260%. Scanning electron microscopy (SEM) images show that there are abundant voids in the material. The cytocompatibility test showed that the material was nontoxic. Conclusion: the polyvinyl alcohol-hyaluronic acid-collagen composite has abundant pore structure and good cytocompatibility, which can be used as scaffold material for tissue engineering.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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