超声辅助酶法去抗原处理猪异种骨的实验研究
发布时间：2019-02-23 11:01
【摘要】：目的采用超声辅助酶法去抗原处理异种骨,期望获得具有免疫原性低、具有良好生物力学性能的理想骨移植材料。方法将猪异种骨用超临界CO2脱脂、深低温冷冻处理后分为3组,分别采用超声波辅助胰蛋白酶法去抗原(A组)、单纯采用超声波法(B组)或胰蛋白酶法(C组)处理异种骨,并取新鲜骨为D组。观察和比较各组的骨材料大体标本和切片、抗原性、材料的生物力学特性。结果超声辅助酶法去抗原处理异种骨速度快,经处理后的猪异种骨材料去抗原更彻底,免疫原性低,孔隙率最高,孔径大小最佳,但材料的生物力学性能有明显下降。结论超声辅助酶法是一种较好的骨材料去抗原处理方法,但处理完后对材料的生物力学性能有明显影响,还需要进一步优化实验参数。
[Abstract]:Objective to obtain an ideal bone graft material with low immunogenicity and good biomechanical properties. Methods Pig xenograft bone was degreased with supercritical CO2 and cryopreserved into 3 groups. The antigens were removed by ultrasonic assisted trypsin method (group A). The heterotopic bone was treated by ultrasonic method (group B) or trypsin method (group C). The antigenicity and biomechanical properties of bone materials were observed and compared. Results Ultrasound-assisted enzymatic antigen-removal of xenogeneic bone was faster, and the treated porcine xenograft material had more complete antigenation, lower immunogenicity, the highest porosity and the best pore size, but the biomechanical properties of the treated porcine xenogeneic bone materials were obviously decreased. Conclusion Ultrasound-assisted enzymatic method is a good antigen-removing method for bone materials, but the biomechanical properties of the materials are significantly affected after the treatment, and further optimization of experimental parameters is needed.
【作者单位】： 贵州省人民医院骨科;
【基金】：贵州省科学技术基金资助项目(黔合科J字[2010]2276号)
【分类号】：R687.3;R318.08
，
本文编号：2428756
[Abstract]:Objective to obtain an ideal bone graft material with low immunogenicity and good biomechanical properties. Methods Pig xenograft bone was degreased with supercritical CO2 and cryopreserved into 3 groups. The antigens were removed by ultrasonic assisted trypsin method (group A). The heterotopic bone was treated by ultrasonic method (group B) or trypsin method (group C). The antigenicity and biomechanical properties of bone materials were observed and compared. Results Ultrasound-assisted enzymatic antigen-removal of xenogeneic bone was faster, and the treated porcine xenograft material had more complete antigenation, lower immunogenicity, the highest porosity and the best pore size, but the biomechanical properties of the treated porcine xenogeneic bone materials were obviously decreased. Conclusion Ultrasound-assisted enzymatic method is a good antigen-removing method for bone materials, but the biomechanical properties of the materials are significantly affected after the treatment, and further optimization of experimental parameters is needed.
【作者单位】： 贵州省人民医院骨科;
【基金】：贵州省科学技术基金资助项目(黔合科J字[2010]2276号)
【分类号】：R687.3;R318.08
，
本文编号：2428756
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