可降解聚羟基烷基酸酯生物弹性体细胞相容性研究
发布时间：2019-02-24 16:42
【摘要】：目的:聚β-羟基丁酸酯(Polyβ-hydroxybutyrate,PHB)是一种由可以由多种细菌发酵得到的脂肪族聚酯,具有良好的生物相容性、生物降解性和压电性等特点。本文旨在检测聚β-羟基丁酸酯聚合物生物弹体部分物理性能以及研究该生物弹体与L-929细胞的生物相容性。方法:本研究首先对聚β-羟基丁酸酯聚乙二醇(PHB-PEG)共聚物进行红外光谱检测,核磁共振图谱分析,示差扫描量热分析,分析该物质物理特性。其次在高温、常温情况下,我们将L-929细胞分别放置于聚β-羟基丁酸酯聚乙二醇聚合物薄膜、聚氯乙烯(PVC)薄膜上培养,观察第二、四、六天L-929细胞存活情况。结果:PHB-PEG共聚物表征检测发现:PHB-PEG红外测试结果证实PHB-PEG之间链接存在;磁共振图谱为PHB-PEG共聚物结构提供了进一步的证明;差示扫描量热显示PHB-PEG不同相之间的转变温度比PHB有所降低。L-929细胞与PHB-PEG共存实验显示:高温、常温等环境条件下,PHB-PEG环境组细胞生长状况良好,较致密,有些细胞突开始伸展,呈梭状;而PV环境组第二天开始出现细胞固缩,死亡。在高温、常温环境中,L-929细胞生长情况在PHB组与阴性对照NaCl组间无显著差异(p0.05);在PHB组与阳性对照组PVC组间有显著差异(p0.01);在阳性对照PVC组与阴性对照NaCl组间有显著差异(p0.01)。同组不同时间点的AO值采用协方差检验,得出阴性对照NaCl组和PHB-PEG组中L-929细胞生长趋势一致,阳性对照组L-929细胞生长呈被抑制状态。L-929细胞能在PHB-PEG多聚物中,细胞毒性评分均为0分,而在PVC环境中,细胞毒性评分为3分;L-929细胞能在PHB-PEG多聚物中环境良好的生长,无细胞毒性产生,生物相容性良好,而L-929细胞在PVC环境中存活较差。结论:PHB-PEG多聚物良好的细胞相容性及其生物性质,使其可能成为潜在医用材料。
[Abstract]:Objective: Poly (尾 -hydroxybutyrate) (Poly 尾 -butyrate) is a kind of aliphatic polyester which can be fermented by a variety of bacteria. It has good biocompatibility, biodegradability and piezoelectric properties. The purpose of this paper is to test the physical properties of poly (尾 -hydroxybutyrate) polymer and to study its biocompatibility with L-929 cells. Methods: in this study, the poly (尾 -hydroxybutyrate) poly (ethylene glycol) (PHB-PEG) copolymers were detected by IR, NMR, DSC, and the physical properties of the copolymers were analyzed. Secondly, L-929 cells were cultured on poly (尾 -hydroxybutyrate) poly (ethylene glycol) polymer film and polyvinyl chloride (PVC) film at high temperature and normal temperature, and the survival of L-929 cells was observed on the second, fourth and sixth day. Results: the results of PHB-PEG copolymer characterization showed that the existence of links between PHB-PEG was confirmed by PHB-PEG IR, and the structure of PHB-PEG copolymers was further proved by Mr spectra. Differential scanning calorimetry (DSC) showed that the transition temperature between different phases of PHB-PEG was lower than that of PHB. The co-existence of L-929 cells and PHB-PEG showed that the cells in PHB-PEG group grew well and denser under high temperature and normal temperature. Some processes began to stretch out, fusiform; In the PV group, cell pyknosis and death began on the second day. There was no significant difference in the growth of L-929 cells between the PHB group and the negative control group (p0.05), but there was significant difference between the PHB group and the positive control group (p0.01) in the growth of L-929 cells in the hyperthermia and normothermic environment. There was significant difference between the positive control PVC group and the negative control NaCl group (p 0.01). The AO values at different time points in the same group were tested by covariance test. The results showed that the growth trend of L-929 cells in NaCl group and PHB-PEG group was the same. In the positive control group, the growth of L-929 cells was inhibited. The cytotoxicity scores of L-929 cells were 0 in PHB-PEG polymers and 3 in PVC. L-929 cells could grow well in the PHB-PEG polymer, had no cytotoxicity and had good biocompatibility, while L-929 cells could not survive in the PVC environment. Conclusion: PHB-PEG polymer has good cytocompatibility and biological properties, which makes it a potential medical material.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R318.08
[Abstract]:Objective: Poly (尾 -hydroxybutyrate) (Poly 尾 -butyrate) is a kind of aliphatic polyester which can be fermented by a variety of bacteria. It has good biocompatibility, biodegradability and piezoelectric properties. The purpose of this paper is to test the physical properties of poly (尾 -hydroxybutyrate) polymer and to study its biocompatibility with L-929 cells. Methods: in this study, the poly (尾 -hydroxybutyrate) poly (ethylene glycol) (PHB-PEG) copolymers were detected by IR, NMR, DSC, and the physical properties of the copolymers were analyzed. Secondly, L-929 cells were cultured on poly (尾 -hydroxybutyrate) poly (ethylene glycol) polymer film and polyvinyl chloride (PVC) film at high temperature and normal temperature, and the survival of L-929 cells was observed on the second, fourth and sixth day. Results: the results of PHB-PEG copolymer characterization showed that the existence of links between PHB-PEG was confirmed by PHB-PEG IR, and the structure of PHB-PEG copolymers was further proved by Mr spectra. Differential scanning calorimetry (DSC) showed that the transition temperature between different phases of PHB-PEG was lower than that of PHB. The co-existence of L-929 cells and PHB-PEG showed that the cells in PHB-PEG group grew well and denser under high temperature and normal temperature. Some processes began to stretch out, fusiform; In the PV group, cell pyknosis and death began on the second day. There was no significant difference in the growth of L-929 cells between the PHB group and the negative control group (p0.05), but there was significant difference between the PHB group and the positive control group (p0.01) in the growth of L-929 cells in the hyperthermia and normothermic environment. There was significant difference between the positive control PVC group and the negative control NaCl group (p 0.01). The AO values at different time points in the same group were tested by covariance test. The results showed that the growth trend of L-929 cells in NaCl group and PHB-PEG group was the same. In the positive control group, the growth of L-929 cells was inhibited. The cytotoxicity scores of L-929 cells were 0 in PHB-PEG polymers and 3 in PVC. L-929 cells could grow well in the PHB-PEG polymer, had no cytotoxicity and had good biocompatibility, while L-929 cells could not survive in the PVC environment. Conclusion: PHB-PEG polymer has good cytocompatibility and biological properties, which makes it a potential medical material.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R318.08
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