多孔磷酸钙骨水泥与转染骨形态发生蛋白2基因的骨髓间充质干细胞复合修复股骨髁骨缺损

发布时间：2019-03-25 12:48

【摘要】：背景:骨形态发生蛋白能够提高组织工程化骨的成骨能力,但如何控制骨形态发生蛋白在机体局部持续缓慢释放,是构建具有骨诱导能力组织工程化骨的关键问题。目的:观察转染骨形态发生蛋白2基因的骨髓间充质干细胞,复合多孔磷酸骨水泥构建组织工程化骨对骨缺损的修复作用。方法:取新西兰兔白12只,制作双侧股骨髁缺损模型,左侧植入转染骨形态发生蛋2基因的骨髓间充质干细胞复合多孔磷酸钙骨水泥组织工程化骨(实验组),右侧植入未转染骨形态发生蛋白2基因的骨髓间充质干细胞复合多孔磷酸钙骨水泥组织工程化骨(对照组)。植入后4,12周取双侧股骨髁,进行组织形态学分析。结果与结论:(1)Van-Gieson染色:植入4周时,实验组可见大量新生的编织骨组织形成,主要集中在材料外周孔隙;对照组仅在材料外周观察到少量新生骨组织。植入12周时,实验组外周编织骨转变为成熟的小梁骨,人工骨内部可见大量新生骨组织形成,外周区域部分支架材料吸收被新生骨组织取代;对照组植入区亦可见较多新生骨组织从材料周边区域向中心区域生长,支架材料吸收不明显;实验组不同时间点的新生骨面积均高于对照组(P0.001);(2)免疫荧光染色:植入12周时,实验组新骨形成速率明显快于对照组(P0.05);(3)结果表明:采用基因转染技术将骨形态发生蛋白2转染至骨髓间充质干细胞并与磷酸钙骨水泥材料复合,可显著提高组织工程化骨修复骨缺损的效果。
[Abstract]:Background: bone morphogenetic protein can improve the osteogenic ability of tissue engineered bone, but how to control the sustained slow release of bone morphogenetic protein in the body is a key problem in the construction of tissue engineered bone with the ability of bone induction. Aim: to observe the effect of bone marrow mesenchymal stem cells transfected with bone morphogenetic protein-2 gene (BMP-2) combined with porous phosphate cement to construct tissue-engineered bone defect. Methods: twelve white New Zealand rabbits were used to establish bilateral femoral condylar defect model. Bone marrow mesenchymal stem cells transfected with bone morphogenetic protein 2 gene and porous calcium phosphate cement tissue engineered bone were implanted on the left side (experimental group). Bone marrow mesenchymal stem cells containing bone morphogenetic protein 2 gene and porous calcium phosphate cement were implanted on the right side of bone marrow mesenchymal stem cells and tissue engineered bone of bone morphogenetic protein 2 gene (control group). At 4 and 12 weeks after implantation, bilateral femoral condyles were taken for histological analysis. Results & conclusion: (1) Van-Gieson staining: at 4 weeks after implantation, a large number of newly formed braided bone tissue was observed in the experimental group, mainly concentrated in the peripheral pores of the material, and only a small amount of new bone tissue was observed in the peripheral part of the material in the control group. At the 12th week after implantation, the peripheral woven bone in the experimental group was transformed into mature trabecular bone. A large number of new bone tissue was formed in the artificial bone, and a part of the scaffold material absorbed in the peripheral region was replaced by the new bone tissue. In the control group, a lot of new bone tissue grew from the surrounding area to the central area, and the bone area of the experimental group was higher than that of the control group (P0.001) at different time points, and there was no obvious absorption of the scaffold material in the implanted area of the control group. (2) immunofluorescence staining: at 12 weeks after implantation, the rate of new bone formation in the experimental group was significantly faster than that in the control group (P0.05). (3) the results showed that bone morphogenetic protein-2 (BMP-2) transfected into bone marrow mesenchymal stem cells and combined with calcium phosphate cement could significantly improve the effect of repairing bone defect with tissue engineered bone.
【作者单位】：宝鸡市中医院骨伤一科;宝鸡市高级职业技术学院;解放军第四军医大学第一附属医院;解放军451医院骨科;
【基金】：国家十一五科技支撑计划(2006BAI16B02)~~
【分类号】：R318.08;R68

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