多孔磷酸钙颗粒对维生素C的负载及其可控释放

发布时间：2019-03-26 08:44

【摘要】：背景:近来有报道指出,维生素C可诱导、加速骨髓间充质干细胞向成骨细胞分化,促进骨的修复和再生,但维生素C水溶液不稳定,因此在其使用时需要载体材料的保护。目的:将磷酸钙作为维生素C的载体,观察其对维生素C的包载及控释能力。方法:采用化学沉淀法制备负载维生素C的磷酸钙颗粒,使维生素C终浓度分别为0,0.1,2,4 mmol/L,检测颗粒载药量。将负载维生素C的磷酸钙颗粒置于模拟体液中,检测维生素C释放量;同时检测超声环境下的维生素C释放量。将磷酸钙颗粒、负载2 mmol/L维生素C的磷酸钙颗粒与MC3T3-E1细胞共培养,1,3,5,7 d后检测细胞增殖,1,5,10,15 d后检测细胞碱性磷酸酶活性。结果与结论:1负载0.1,2,4 mmol/L维生素C磷酸钙颗粒的载药量分别为(59.9±5.4)%、(87.2±1.2)%及(28.4±26.3)%;2在正常环境下,负载0.1,2 mmol/L维生素C的磷酸钙颗粒前期释放速率较慢,负载4 mmol/L维生素C的磷酸钙颗粒前期释放速率较快,3种样品都有持续释药的特点;3在超声环境下,负载2 mmol/L维生素C的磷酸钙颗粒存在一定的突释现象,第1次释放量为5%-15%,而随后的释放速度较缓慢;在75,105和150 W超声辅助条件下,分别持续释放了220,340,260 min;4负载2 mmol/L维生素C的磷酸钙颗粒对MC3T3-E1细胞的增殖无影响,但提高了细胞的碱性磷酸酶活性;5结果表明,磷酸钙可作为维生素C的载体。
[Abstract]:Background: it has been reported recently that vitamin C can induce and accelerate the differentiation of bone marrow mesenchymal stem cells into osteoblasts and promote bone repair and regeneration. However, vitamin C solution is unstable, so it needs the protection of carrier materials in its use. Objective: to observe the encapsulation and controlled release ability of calcium phosphate as carrier of vitamin C. Methods: calcium phosphate granules loaded with vitamin C were prepared by chemical precipitation method. The final concentrations of vitamin C were 0, 0.1, 2 and 4 mmol/L, respectively. Calcium phosphate particles loaded with vitamin C were placed in simulated body fluid to detect vitamin C release and vitamin C release in ultrasonic environment. MC3T3-E1 cells were co-cultured with calcium phosphate granules loaded with 2 mmol/L vitamin C for 1, 3, 5, 7 days to detect cell proliferation, 1,5,10,15 days later to detect the activity of alkaline phosphatase (ALP). Results and conclusion: 1the loading amounts of vitamin C calcium phosphate granules at 0.1, 2 and 4 mmol/L were (59.9 卤5.4)%, (87.2 卤1.2)% and (28.4 卤26.3)%, respectively. (2) in normal environment, the release rate of calcium phosphate granules loaded with 2 mmol/L vitamin C was slower than that of calcium phosphate granules loaded with 4 mmol/L vitamin C at 0.1, and all three samples had the characteristics of sustained drug release, and the release rate of calcium phosphate granules loaded with 4 mmol/L vitamin C was faster than that of calcium phosphate granules loaded with 4 mmol/L vitamin C. 3There was a sudden release of calcium phosphate granules loaded with 2 mmol/L vitamin C in ultrasonic environment, the first release rate was 5% ~ 15%, but the subsequent release rate was slower; Under 75105 and 150W ultrasound-assisted conditions, calcium phosphate particles loaded with 2 mmol/L vitamin C at 220340260 min;4 had no effect on the proliferation of MC3T3-E1 cells, but enhanced the alkaline phosphatase activity of MC3T3-E1 cells. 5 the results showed that calcium phosphate could be used as carrier of vitamin C.
【作者单位】：浙江省立同德医院;浙江省中医药研究院;
【分类号】：R318

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