鱼皮胶原纤维重组构建纳吸棉的工艺特征

发布时间：2019-04-02 19:18

【摘要】：背景:鱼源胶原基医用生物材料在热稳定性、体内降解稳定性以及体内材料形态学稳定性等方面仍存在明显不足,利用技术手段提高淡水鱼类胶原海绵材料的密度和胶原分子间结合的紧密程度进而提高材料性能以满足应用需要成为拓展鱼源胶原应用领域的当务之急。目的:探究鱼皮胶原纤维重组构建纳吸棉的工艺条件。方法:以罗非鱼鱼皮为原料,在低于胶原蛋白变性温度的条件下提取酶促溶性胶原蛋白,以胶原蛋白纤维重组率为考察指标,研究胶原蛋白纤维重组工艺并对其进行参数优化,确定了鱼皮胶原纤维重组构建纳吸棉的最佳工艺。以此为基础进行重组纳吸棉的红外光谱和微观结构分析。结果与结论:通过单因素试验和正交试验得出最佳鱼皮胶原纤维重组反应条件为:胶原质量浓度为1 g/L,离子强度(Na Cl浓度)为65 mmol/L,p H值7.4,体系温度为20℃,重组时间为10 h,在该条件下得到的纤维重组率高达68.6%。在此基础上制备的纳吸棉具有细线状纤维构成的多孔网状结构,且其三螺旋结构完整,是一种性能优良的腔内止血材料。
[Abstract]:Background: there are still obvious deficiencies in thermal stability, degradation stability and morphological stability of fish-derived collagen-based biomaterials. It is an urgent task to improve the density of freshwater fish collagen sponge materials and the density of collagen molecules and to improve the properties of the materials to meet the needs of the application of fish collagen. Objective: to explore the technological conditions of constructing nano-absorbent cotton by recombination of collagen fiber from fish skin. Methods: using tilapia skin as raw material, the enzyme-enhanced collagen was extracted under the condition of lower than the denaturation temperature of collagen. The recombination rate of collagen fiber was used as the index to study the technology of collagen fiber recombination and its parameters were optimized. The optimum technology of recombining fish skin collagen fiber to construct nano-absorbent cotton was determined. On the basis of this, the infrared spectrum and microstructure analysis of the reconstituted nano-absorbent cotton were carried out. Results and conclusion: the optimum reaction conditions of fish skin collagen fiber recombination were as follows: collagen concentration was 1 g / L and ionic strength (Na Cl concentration was 65 mmol/L,p H = 7.4, and the optimal reaction conditions were as follows: collagen concentration was 1 g / L and ionic strength concentration was 7.4mol 路L ~ (- 1). When the temperature of the system was 20 鈩，

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