补骨脂素抗菌双相陶瓷骨的制备及性能检测

发布时间：2019-04-04 08:13

【摘要】：背景:近年来中药在骨组织工程中应用的研究在国内处于起步阶段,尚未见到补骨脂素在骨组织工程支架材料中应用的研究报道。目的:通过真空复合方法制备出补骨脂素抗菌双相陶瓷骨,检测材料的理化性能、抗菌性和细胞相容性。方法:将猪椎骨经复合焦磷酸钠两次低温煅烧制备双相陶瓷骨,将双相陶瓷骨置于壳聚糖/补骨脂素复合溶液中,制备补骨脂素抗菌双相陶瓷骨。扫描电镜观察补骨脂素抗菌双相陶瓷骨的表面形貌,并检测其抗压强度;采用微生物法检测补骨脂素抗菌双相陶瓷骨对大肠杆菌、金黄色葡萄球菌的抑制效果;将补骨脂素抗菌双相陶瓷骨与小鼠成骨细胞MC3T3-El共培养4,8 d,扫描电镜观察细胞在材料表面的附着与增殖。结果与结论:补骨脂素抗菌双相陶瓷骨具有天然的孔隙结构,骨小梁、小梁间隙和骨内管腔系统同时存在,抗压强度为(4.69±0.50)MPa,其对大肠杆菌、金黄色葡萄球菌均有较强的抑制作用,抑菌效果明显;小鼠成骨细胞MC3T3-El可在补骨脂素抗菌双相陶瓷骨上很好的附着,保持良好的状态和功能并快速增殖,说明补骨脂素抗菌双相陶瓷骨具有良好的细胞相容性。
[Abstract]:Background: the application of traditional Chinese medicine in bone tissue engineering has been in its infancy in recent years, and the application of psoralen in bone tissue engineering scaffolds has not been reported. Aim: to prepare psoralen antibacterial biphasic ceramic bone by vacuum composite method, and detect the physical and chemical properties, antibacterial and cellular compatibility of the material. Methods: porcine vertebrae were calcined with sodium pyrophosphate for two times to prepare biphasic ceramic bone. The biphasic ceramic bone was placed in chitosan / psoralen composite solution to prepare psoralen antibacterial biphasic ceramic bone. The surface morphology and compressive strength of psoralen antibacterial biphasic ceramic bone were observed by scanning electron microscope (SEM), and the inhibitory effect of psoralen antibacterial biphasic ceramic bone on Escherichia coli and Staphylococcus aureus was detected by microbiological method. The bone of psoralen antibacterial biphasic ceramic was co-cultured with mouse osteoblast MC3T3-El for 4 days and 8 days, and the adhesion and proliferation of cells on the surface of the material were observed by scanning electron microscope (SEM). Results & conclusion: psoralen antibacterial biphasic ceramic bone has a natural pore structure. The bone trabecular, trabecular space and endovascular system exist at the same time. The compressive strength is (4.69 卤0.50) MPa, for E. coli. Staphylococcus aureus had a strong inhibitory effect, and the bacteriostatic effect was obvious. Mouse osteoblast MC3T3-El can adhere to psoralen antibacterial biphasic bone, maintain good state and function, and proliferate rapidly, indicating that psoralen antibacterial biphasic bone has good cellular compatibility.
【作者单位】：青岛市海慈医疗集团关节科;青岛市黄岛区第二人民医院;
【分类号】：R318.08

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