胶原蛋白-硫酸肝素生物支架在猪脑内的生物相容性分析

发布时间：2019-04-07 16:44

【摘要】：背景:胶原蛋白-硫酸肝素支架与人体神经内部结构十分相似。目的:探讨胶原蛋白-硫酸肝素支架的体内生物相容性。方法:将40只小猪随机均分为两组,以距离前囟中线旁开1.0 cm为穿刺点,利用骨穿针进行纵向穿刺,至脑蛛网膜下腔,感到落空感后,将骨穿针缓慢拔除,观察组植入胶原蛋白-硫酸肝素支架,对照组不予处置。术后1,3,7,14,30 d对两组脑组织进行透射电镜观察、细胞凋亡及Caspase-3蛋白表达检测。结果与结论:早期电镜观察发现,观察组支架周边有个别脑组织神经元受损情况,并出现髓神经纤维脱髓鞘变化,支架与脑组织交界处及支架内部存在阳性Caspase-3蛋白,但在远离支架周边脑组织则无阳性蛋白表达。从术后1 d开始,观察组支架周边脑组织出现个别脑细胞凋亡情况,至术后30 d无明显凋亡细胞,对照组也表现出相似的变化,观察组术后1,3,7,14 d细胞凋亡数量高于对照组(P0.05),两组术后30 d细胞凋亡数量比较差异无显著性意义(P0.05)。两组Caspase-3蛋白表达均处于较低状态,且观察组术后3,7 d的Caspase-3蛋白表达高于对照组(P0.05),其余时间点比较差异无显著性意义。表明胶原蛋白-硫酸肝素支架在猪脑内具有良好的生物相容性。
[Abstract]:Background: the collagen-heparin sulfate scaffold is very similar to the internal structure of human nerve. Objective: to investigate the biocompatibility of collagen-heparin sulfate scaffold in vivo. Methods: 40 piglets were randomly divided into two groups. The puncture point was 1.0 cm from the midline of the anterior fontanelle, and the bone puncture needle was used to puncture the subarachnoid space of the brain. After feeling emptiness, the bone puncture needle was removed slowly. The collagen-heparin sulfate stent was implanted in the observation group, and the control group was not disposed of. At 1, 3, 7, 14, 30 days after operation, the brain tissues of the two groups were observed by transmission electron microscope (TEM), and the expression of Caspase-3 protein and apoptosis were detected. Results & conclusion: in the observation group, there were some brain tissue neurons damaged around the stent and demyelinating change of myelin nerve fiber. There were positive Caspase-3 protein in the junction between the stent and brain tissue and inside the stent, and there was no significant difference between the stent and the brain tissue between the stent and the stent. However, there was no positive protein expression in the brain tissue far from the stent. From the 1st day after the operation, some brain cells apoptosis appeared in the brain around the stent in the observation group, and there were no obvious apoptotic cells in the brain tissue around the stent in the observation group 30 days after the operation. The changes in the control group were similar to those in the control group. The number of apoptosis on the 14th day was higher than that in the control group (P0.05). There was no significant difference in the number of apoptotic cells between the two groups on the 30th day after operation (P0.05). The expression of Caspase-3 protein was lower in the two groups, and the expression of Caspase-3 protein in the observation group was higher than that in the control group on the 3rd and 7th day after operation (P0.05), but there was no significant difference in the other time points. The results showed that collagen-heparin sulfate scaffold had good biocompatibility in pig brain.
【作者单位】：黄石市中心医院(湖北理工学院附属医院)神经内科;黄石市中心医院(湖北理工学院附属医院)肾脏疾病发生与干预湖北省重点实验室;肾脏疾病发生与干预湖北省重点实验室;黄石市中心医院(湖北理工学院附属医院)药学部;佳木斯大学附属第一医院神经一科;
【分类号】：R318.08

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