不同方法制备异种骨支架材料的生物相容性研究

发布时间：2019-04-30 18:34

【摘要】：目的：检测3种不同方法制备异种骨支架材料的生物相容性。方法：通过急性毒性实验、热源实验、皮内刺激实验、皮下植入实验和细胞毒性实验，初步评价经过物理、化学及物理联合化学不同处理方法制备的羊椎骨异种骨材料的生物相容性。结果：急性毒性实验：物理、物理联合化学和对照组动物7d内无毒性反应；化学组动物初期出现呼吸困难轻度症状，，但无死亡情况。说明物理、物理联合化学组材料无毒性；化学组材料有轻度毒性。热源实验：物理、化学及物理联合化学组实验前后动物体温变化差值有统计学差异（P＜0.05）。说明物理、物理联合化学组材料不具有致热源作用；化学组材料存在致热源作用。皮内刺激实验：物理、物理联合化学组注射处皮肤无明显红斑、水肿和坏死，说明物理、物理联合化学组材料对皮肤无刺激性；化学组注射处皮肤在48、72h时出现极轻微红斑，表明化学组材料有轻度刺激性。皮下植入实验：组织学观察发现物理联合化学组材料周围发现有少量成骨细胞规则排列，同时可见少量成骨，表明有异位成骨的趋势；物理组和化学组材料周围未见成骨细胞排列。细胞毒性实验：MTT法证实物理、化学、物理联合化学组材料浸液和对照组培养骨髓间充质干细胞12d内，除物理、物理联合化学组及对照组复合培养液的吸光度值比较无统计学差异（P＞0.05）外，其他各组间均有统计学差异（P＜0.05）。细胞能在物理、物理联合化学组材料上良好地黏附、增殖、生长；细胞在化学组材料上生长受到抑制，材料孔隙内无细胞黏附。结论：经过物理联合化学方法处理的异种骨材料具有良好的生物相容性；单纯经过物理或化学方法处理的异种骨材料生物相容性较差，不符合生物材料安全性标准。
[Abstract]:Objective: to investigate the biocompatibility of xenogeneic bone scaffolds prepared by three different methods. Methods: acute toxicity test, heat source test, intradermal stimulation test, subcutaneous implantation test and cytotoxicity test were carried out. Biocompatibility of different bone materials of sheep vertebrae prepared by chemical and physical methods. Results: acute toxicity test: physical, physical combined chemistry and control group had no toxic reaction within 7 days, the animals in the chemical group had mild symptoms of dyspnea at the initial stage, but there was no death. Physical and physical combination of chemical group materials are non-toxic; chemical group materials have mild toxicity. Heat source experiment: the difference of animal body temperature before and after physical, chemical and physical combined chemistry group had statistical difference (P < 0.05). The results show that the physical and physical chemical composition materials have no heat source effect, but the chemical composition materials have the heat source effect. Intradermal stimulation test: there was no obvious erythema, edema and necrosis in the injection site of physical and physical combined chemical group, which indicated that physical and physical combined chemical group had no irritation to the skin; The skin of the chemical group showed slight erythema at 48 h and 72 h, indicating that the chemical group had mild irritation. Subcutaneous implantation experiment: histological observation found that a small number of osteoblasts arranged regularly around the material in the physical combined chemical group, and a small amount of osteogenesis could be seen, indicating the tendency of heterotopic osteogenesis. No osteoblast arrangement was observed around the physical and chemical groups. Cytotoxicity test: MTT method confirmed physical, chemical, physical combined with chemical group material extract and control group cultured bone marrow mesenchymal stem cells within 12 days, except physics, There was no significant difference in the absorbance value between the physical combined chemical group and the control group (P > 0.05), but there was statistical difference among the other groups (P < 0.05). The cells could adhere, proliferate and grow well in the physical and physical groups, and the growth of the cells in the chemical group was inhibited, and there was no cell adhesion in the pores of the materials. Conclusion: the heterogenous bone materials treated by physical and chemical methods have good biocompatibility, and the heterogenous bone materials treated by physical or chemical methods alone have poor biocompatibility, which does not meet the safety standard of biomaterials.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R318.08

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