不同剂量氯化镧对氧化铝陶瓷颗粒诱导RAW264.7细胞产生炎症反应的保护作用

发布时间：2019-05-08 20:40

【摘要】：目的观察不同剂量氯化镧(LaCl3)对氧化铝(Al2O3)陶瓷颗粒诱导的小鼠巨噬细胞RAW264.7产生炎症反应的作用效果,为研究LaCl3在Al2O3陶瓷颗粒诱导无菌性松动中的作用提供理论基础。方法体外培养小鼠巨噬细胞RAW264.7。根据培养液的不同分成8组:空白对照组(培养基中不含LaCL3和Al2O3)、Al2O3组(含1 mg/mL Al2O3)、LaCl3+Al2O3组(加入1 mg/mL Al2O3后分别加入2.5、10、100μmol/L LaCl3)以及LaCl3组(含2.5、10、100μmol/L LaCl3)。分别采用ELISA、RT-PCR及Western blotting法检测炎症因子白介素1β(IL-1β)、肿瘤坏死因子-α(TNF-α)、核因子-κB(NF-κB)mRNA和蛋白的表达。结果 ELISA检测结果显示:与空白对照组比较,Al2O3组和100μmol/L LaCl3组IL-1和TNF-β的蛋白分泌量均显著升高,差异有统计学意义(P0.05);与Al2O3组比较,Al2O3+2.5μmol/L LaCl3组、Al2O3+10μmol/L LaCl3组均显著降低,差异有统计学意义(P0.05);2.5和10μmol/L LaCl3组均明显低于100μmol/L LaCl3组,差异也有统计学意义(P0.05)。RT-PCR和Western blotting检测结果显示IL-1β、TNF-α、NF-κB mRNA以及NF-κB蛋白表达的变化趋势与ELISA检测结果一致。结论 10μmol/L LaCl3可在一定程度上抑制巨噬细胞IL-1β和TNF-α的分泌。LaCl3的炎症抑制作用可能与NF-κB信号转导通路的抑制有关。
[Abstract]:Aim to observe the effect of different doses of lanthanum chloride (LaCl3) on the inflammatory response of mouse macrophage RAW264.7 induced by alumina (Al2O3) ceramic particles, and to provide a theoretical basis for studying the role of LaCl3 in aseptic loosening induced by Al2O3 ceramic particles. Methods Mouse macrophage RAW264.7. was cultured in vitro. According to the different culture medium, they were divided into 8 groups: blank control group (without LaCL3 and Al2O3 in the culture medium), Al2O3 group (containing 1 mg/mL Al2O3), LaCl3 Al2O3 group (2.5100 渭 mol / L LaCl3 after adding 1 mg/mL Al2O3) and LaCl3 group (containing 2.5100 渭 mol / L LaCl3). The expression of IL-1 尾, TNF- 伪, NF- 魏 B mRNA and protein were detected by ELISA,RT-PCR and Western blotting respectively. Results the results of ELISA showed that the protein secretion of IL-1 and TNF- 尾 in Al2O3 group and 100 渭 mol / L LaCl3 group were significantly higher than those in blank control group (P0.05). Compared with Al2O3 group, Al2O3 2.5 渭 mol / L LaCl3 group and Al2O3 10 渭 mol / L LaCl3 group decreased significantly (P 0.05). The levels of IL-1 尾 and TNF- 伪 in LaCl3 group were significantly lower than those in 100 渭 mol / L LaCl3 group (P0.05). The results of RT-PCR and Western blotting showed that IL-1 尾 and TNF- 伪 were significantly lower than that of 100 渭 mol / L Western blotting group (P0.05). The expression trend of NF- 魏 B mRNA and NF- 魏 B protein was consistent with that of ELISA. Conclusion 10 渭 mol / L LaCl3 can inhibit the secretion of IL-1 尾 and TNF- 伪 in macrophages to a certain extent, and the inflammatory inhibition of Lacl 3 may be related to the inhibition of NF- 魏 B signal transduction pathway.
【作者单位】：南昌大学第一附属医院骨科;
【分类号】：R318.17

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