多孔钛铌合金表征及生物相容性的实验研究
发布时间：2019-06-04 15:13
【摘要】：研究目的：人工关节无菌性松动是当前导致人工关节置换失败的主要原因之一,而获得骨-植入物界面的稳定又是防止人工关节无菌性松动的关键所在。骨-植入物界面的稳定取决于两者的结合强度,即骨整合的效果,这与植入物的力学性能和表面特征密切相关。钛具有较好的物理特性和良好的生物相容性,而铌可以提高钛合金的可加工性,且能增加钛的强度,降低弹性模量。多孔表面设计有利于骨组织的长入,随着骨组织长入到金属植入物的多孔结构内部,获得植入物和骨的结合,从而提供良好的生物学固定。本研究利用粉末冶金技术研制具有高孔隙率(60%)和理想力学强度的多孔钛铌合金,从生物力学、孔径及孔隙率、体外生物相容性及体内骨整合效果等方面对该合金进行评估,以了解多孔钛铌合金的性能,为多孔钛铌合金的临床应用提供基础实验依据。 方法：1.采用粉末冶金技术制作多孔钛铌合金并对其生物力学强度、孔径及孔隙率等进行评估；2.体外生物相容性检测,包括细胞毒性试验(MTT),细胞粘附和增殖试验(丫啶橙染色及扫描电镜),炎症因子检测(IL-6)等；3.动物体内骨整合检测,将设计好的合金试样植入新西兰大白兔股骨近端髓腔,分别于2,4,8周行X线摄片、扫描电镜、能谱分析及力学拔出实验。 结果：1.70%孔隙率多孔钛铌合金力学强度(抗压强度为94.8MPa,弹性模量为2.23GPa)和松质骨接近；2.70%孔隙率多孔钛铌合金孔径约为200-500μm,孔隙连通；3.MTT实验显示兔骨髓间充质干细胞对钛铌合金无毒性反应,材料毒性级别为0-1级；4.细胞粘附和增殖试验显示钛铌合金对兔骨髓间充质干细胞初期粘附和增殖无明显影响,电镜扫描见细胞在多孔钛铌合金材料表面生长良好,并向多孔材料孔隙内生长；5.炎症因子(IL-6)检测显示多孔钛铌合金未引起明显炎症反应；6.新西兰大白兔股骨近端髓腔多孔钛铌合金植入显示材料植入动物体内未引起明显炎症反应,多孔钛铌合金植入体无松动；7.X线摄片显示植入体与股骨皮质之间无低密度影；8.扫描电镜及能谱分析显示材料表面有较多钙沉积,植入体内8周材料与骨紧密结合,类骨组织向材料孔隙内生长,骨整合效果良好,70%孔隙率多孔钛铌合金较40%孔隙率组骨整合效果好；9.力学拔出实验显示70%孔隙率组和40%孔隙率组拔出力量无显著性差异(P0.05),但较致密组拔出力量大(P0.05)。 结论： 1.高孔隙率多孔钛铌合金具有较理想的抗压强度和弹性模量； 2.钛铌合金具有良好的生物相容性； 3.高孔隙率多孔钛铌合金-骨界面骨整合效果良好。
[Abstract]:Objective: aseptic loosening of artificial joint is one of the main reasons for the failure of artificial joint replacement, and the stability of bone-implantation interface is the key to prevent aseptic loosening of artificial joint. The stability of the interface between bone and implantation depends on the binding strength of the two, that is, the effect of bone integration, which is closely related to the mechanical properties and surface characteristics of the implantation. Titanium has good physical properties and good biocompatibility, while niobium can improve the machinability of titanium alloy, increase the strength of titanium and reduce the elastic modulus. The design of porous surface is beneficial to the growth of bone tissue. As the bone tissue grows into the porous structure of metal implantation, the combination of implantation and bone can be obtained, thus providing good biological fixation. In this study, porous titanium-niobium alloys with high porosity (60%) and ideal mechanical strength were prepared by powder metallurgy technology. The biocompatibility in vitro and the effect of bone integration in vivo were evaluated in order to understand the properties of porous titanium-niobium alloy and to provide basic experimental basis for the clinical application of porous titanium-niobium alloy. Methods: 1. Porous titanium-niobium alloy was fabricated by powder metallurgy technology and its biomechanical strength, pore size and porosity were evaluated. Biocompatibility test in vitro, including cytotoxicity test (MTT), cell adhesion and proliferation test (acacia orange staining and scanning electron microscope), inflammatory factor detection (IL-6), etc. The designed alloy samples were implanted into the proximal pulp cavity of New Zealand white rabbits. X-ray film, scanning electron microscope (SEM), energy spectrum analysis and mechanical pullout test were performed at 2, 4 and 8 weeks, respectively. the results were as follows: (1) X-ray film, scanning electron microscope (SEM), energy spectrum analysis (EDS) and mechanical pullout test. Results: the mechanical strength (compressive strength 94.8 MPA, elastic modulus 2.23GPa) of 1.70% porosity porous titanium-niobium alloy was close to that of cancellous bone, and the pore size of 2.70% porosity porous titanium-niobium alloy was about 200-500 渭 m, and the pore size of porous titanium-niobium alloy was about 200-500 渭 m, and the porosity of porous titanium-niobium alloy was about 200-500 渭 m. 3.MTT test showed that rabbit bone marrow mesenchymal stem cells had no toxic reaction to titanium and niobium alloy, and the toxicity grade of the material was 0 鈮，
本文编号：2492808
[Abstract]:Objective: aseptic loosening of artificial joint is one of the main reasons for the failure of artificial joint replacement, and the stability of bone-implantation interface is the key to prevent aseptic loosening of artificial joint. The stability of the interface between bone and implantation depends on the binding strength of the two, that is, the effect of bone integration, which is closely related to the mechanical properties and surface characteristics of the implantation. Titanium has good physical properties and good biocompatibility, while niobium can improve the machinability of titanium alloy, increase the strength of titanium and reduce the elastic modulus. The design of porous surface is beneficial to the growth of bone tissue. As the bone tissue grows into the porous structure of metal implantation, the combination of implantation and bone can be obtained, thus providing good biological fixation. In this study, porous titanium-niobium alloys with high porosity (60%) and ideal mechanical strength were prepared by powder metallurgy technology. The biocompatibility in vitro and the effect of bone integration in vivo were evaluated in order to understand the properties of porous titanium-niobium alloy and to provide basic experimental basis for the clinical application of porous titanium-niobium alloy. Methods: 1. Porous titanium-niobium alloy was fabricated by powder metallurgy technology and its biomechanical strength, pore size and porosity were evaluated. Biocompatibility test in vitro, including cytotoxicity test (MTT), cell adhesion and proliferation test (acacia orange staining and scanning electron microscope), inflammatory factor detection (IL-6), etc. The designed alloy samples were implanted into the proximal pulp cavity of New Zealand white rabbits. X-ray film, scanning electron microscope (SEM), energy spectrum analysis and mechanical pullout test were performed at 2, 4 and 8 weeks, respectively. the results were as follows: (1) X-ray film, scanning electron microscope (SEM), energy spectrum analysis (EDS) and mechanical pullout test. Results: the mechanical strength (compressive strength 94.8 MPA, elastic modulus 2.23GPa) of 1.70% porosity porous titanium-niobium alloy was close to that of cancellous bone, and the pore size of 2.70% porosity porous titanium-niobium alloy was about 200-500 渭 m, and the pore size of porous titanium-niobium alloy was about 200-500 渭 m, and the porosity of porous titanium-niobium alloy was about 200-500 渭 m. 3.MTT test showed that rabbit bone marrow mesenchymal stem cells had no toxic reaction to titanium and niobium alloy, and the toxicity grade of the material was 0 鈮，
本文编号：2492808
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