高压静电法结合冷冻干燥法和离子交联法制备壳聚糖-明胶多孔微球

发布时间：2019-07-24 09:19

【摘要】：目的·应用高压静电法结合冷冻干燥(冻干)法和离子交联法制备壳聚糖明胶多孔微球,探讨微球多孔结构形成的影响因素。方法·以三聚磷酸钠(STPP)为交联剂,利用高压静电法结合冻干法和离子交联法制备具有多孔结构的壳聚糖多孔微球和壳聚糖明胶多孔微球,比较壳聚糖明胶不同体积比、冷冻温度、饱和STPP的85%乙醇溶液交联固化时间对微球多孔结构的影响,光镜、扫描电镜、苏木精-伊红染色切片观察壳聚糖多孔微球和壳聚糖明胶多孔微球的形态、表面及内部结构、粒径。结果·高压静电结合冻干法和离子交联法制备的微球,球形良好,球体表面及内部均有较多微孔,表面与内部孔径相通。饱和STPP的85%乙醇溶液交联固化后的壳聚糖明胶微球结构能在中性溶液中稳定存在,冷冻、冻干与交联反应的先后顺序,交联时间及二次冷冻温度影响多孔微球的孔径。明胶可以和壳聚糖形成聚电解质复合物,也可以作为致孔剂参与多孔结构的形成。结论·高压静电法结合冻干法和离子交联法制备的壳聚糖明胶多孔微球具有较大的孔径(100~200μm),适合细胞的生长及迁移。
[Abstract]:Objective To study the influence factors of the porous structure of the microspheres by using the high-voltage electrostatic method in combination with the freeze-drying (freeze-drying) method and the ion cross-linking method to prepare the chitosan-gelatin porous microspheres. The method comprises the following steps: using sodium tripolyphosphate (STPP) as a cross-linking agent, and preparing a chitosan porous microsphere and a chitosan gelatin porous microsphere with a porous structure by using a high-voltage electrostatic method in combination with a freeze-drying method and an ion cross-linking method, and comparing the different volume ratio and the freezing temperature of the chitosan gelatin, The morphology, surface and internal structure and particle size of the porous microspheres and the chitosan-gelatin porous microspheres were observed by light microscope, scanning electron microscope and hematoxylin-eosin staining. Results The microspheres prepared by the method of high-voltage static electricity combined with the freeze-drying method and the ion cross-linking method have good spherical shape, and the surface of the sphere and the inner part of the microsphere have more micropores, and the surface is communicated with the internal pore. The cross-linking time and the secondary freezing temperature influence the pore size of the porous microspheres in the order of the stable existence, the freezing, the freeze-drying and the cross-linking reaction in the neutral solution after the crosslinking and curing of the 85% ethanol solution of the saturated STPP. The gelatin can form a polyelectrolyte complex with the chitosan, and can also be used as a pore-forming agent to participate in the formation of the porous structure. Conclusion The chitosan-gelatin porous microspheres prepared by the combination of freeze-drying and ion-crosslinking have a large pore size (100-200. mu.m), which is suitable for cell growth and migration.
【作者单位】：上海交通大学医学院附属瑞金医院泌尿外科;上海交通大学医学院附属瑞金医院普外科;
【基金】：国家高新技术研究发展计划(“863”计划)(2008AA02Z417) 国家自然科学基金(30672043,31170938)~~
【分类号】：R318.08

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