Electrospun Nanofibers of Hydroxyapatite / Collagen / Chitos

发布时间：2021-05-18 05:54

　　Bone tissue engineering, aiming at developing bone substitutes for repair and regeneration of bone defects instead of using autologous bone grafts,has attracted wide attention in the field of tissue engineering and regenerative medicine.Developing biomimetic biomaterial scaffolds able to regulate osteogenic differentiation of stem cells could be a promising strategy to improve the therapeutic efficacy.In this study, electrospun composite nanofibers of hydroxyapatite / collagen / chitosan（ HAp / ... 

【文章来源】：Journal of Donghua University（English Edition）. 2014,31(05)EI

【文章页数】：5 页

【文章目录】：
Introduction
1 Experimental
    1.1 Materials
    1.2 Fabrication of HAp/Col/CTS nanofibers
    1.3 Cell culture with scaffolds
    1.4 Cell proliferation assay
    1.5 Alkaline phosphatase (ALP) activity
    1.6 Col quantification assay
    1.7 Biomineralization assay
    1.8 Statistical analysis
2 Results and Discussion
    2.1 Morphology observation
    2.2 Proliferation of BMSCs
    2.3 Osteogenic differentiation of BMSCs
    2.4 Biomineralization of the differentiated osteoblasts
3 Conclusions



本文编号：3193243