SHH缓释纤维蛋白支架移植促大鼠脊髓损伤的修复

发布时间：2018-07-03 17:27

本文选题：脊髓损伤 + 音猬因子　；参考：《神经解剖学杂志》2017年02期

【摘要】：目的:研究应用可缓释音猬因子(sonic hedgehog,SHH)的纤维蛋白胶(fibrin glue,FG)支架促大鼠脊髓完全横断损伤的修复效果。方法:(1)制作体外缓释SHH的纤维蛋白胶支架模型,探究缓释效果。(2)选用健康的SD大鼠60只,制作大鼠脊髓完全横断模型,随机分为三组:单纯脊髓横断组(SCI),纤维蛋白胶组(FG),音猬因子-支架移植组(F-SHH)。术后每周记录大鼠双后肢功能活动(BBB评分)。术后12周取出脊髓组织,采用免疫组织化学染色和免疫蛋白印迹检测,观察脊髓损伤的近远端神经元存活情况及神经丝蛋白(neurofilament200,NF200),生长再生相关蛋白(growth associated protein-43,GAP43),胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)表达情况。结果:(1)载SHH的纤维蛋白胶具有良好的缓释SHH的效果;(2)F-SHH组BBB评分从术后到第十二周较FG、SCI组上升明显(P0.05);(3)免疫组化结果显示:F-SHH组脊髓横断端有一些神经元存活且排列规则,许多神经纤维未完全溃变。SCI组细胞凋亡明显,纤维溃变严重,退变空泡大,FG组较SCI组稍好;(4)Western Blot结果显示:F-SHH组NF200,GAP43相对含量高于FG、SCI组(P0.05),而GFAP相对含量低于FG、SCI组(P0.05)。结论:SHH缓释纤维蛋白支架缓释效果良好,对大鼠脊髓完全横断损伤的修复有明显的促进作用,SHH对于脊髓损伤的修复机理值得进一步研究。
[Abstract]:Aim: to study the effect of sonic hedgehog factor (sonic hedgehog) on the repair of spinal cord complete transection injury (sci) in rats with fibrin glueosin (FG) scaffold. Methods: (1) the fibrin gel scaffold model of sustained release of SHH in vitro was made, and the effect of sustained release was investigated. (2) 60 healthy SD rats were selected to make the spinal cord transection model. They were randomly divided into three groups: spinal cord transection group (sci), fibrin glue group (FG) and hedgehog factor-stent graft group (F-SHH). The functional activities of the hind limbs (BBB score) were recorded weekly after operation. Spinal cord tissues were taken out 12 weeks after operation and were detected by immunohistochemistry and Western blot. The survival of proximal and distal neurons and the expression of neurofilament200NF-200, (growth associated protein-43 and (glial fibrillary acidic protein of glial fibrillary acidic protein (GFAP) were observed. Results: (1) the fibrin glue loaded with SHH had a good effect of sustained release of SHH. (2) the BBB score of F-SHH group was significantly higher than that of FGG sci group from postoperative to 12th week (P0.05); (3) the immunohistochemical results showed that some neurons in the transverse end of spinal cord in the FSH group survived and arranged regularly. The results of Western blot showed that the relative content of NF200 GAP43 in the FG group was higher than that in the FGSCI group (P0.05), and the relative content of GFAP in the FG group was lower than that in the FGSCI group (P0.05). Conclusion the sustained release effect of% SHH fibrin scaffold is good and can promote the repair of spinal cord complete transection injury in rats. The mechanism of SHH on spinal cord injury should be further studied.
【作者单位】：江苏大学附属医院骨科;江苏大学医学院;
【基金】：国家自然科学基金(51403086) 江苏省自热科学基金(BK20140544) 江苏大学高级人才启动基金(13JDG089) 镇江市社会发展科技基金(SH2012026)
【分类号】：R651.2

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