姜黄素对大鼠退行性腰椎间盘NF-κB表达的影响

发布时间：2018-07-04 19:45

本文选题：姜黄素 + 退行性腰椎间盘　；参考：《宁夏医科大学》2015年硕士论文

【摘要】：目的观察姜黄素对大鼠退行性腰椎间盘髓核中核转录因子-kappa B-p65(NF-κB-p65)、肿瘤坏死因子-alpha(TNF-a)表达的影响并且探讨其延缓椎间盘退行性改变进程机制的研究。方法建立Sprague-Dawley(SD)大鼠腰椎间盘退行性改变模型,给予姜黄素治疗,超显微组织形态学变化评价SD大鼠腰椎间盘退行性改变,分别检测退变的椎间盘组织中NF-κB-p65与TNF-a在蛋白和基因的表达。结果1.通过腰椎核磁共振(MRI)和组织形态学证实大鼠腰椎间盘退变模型建立成功;2.通过MRI和超显微结果发现姜黄素治疗组可以明显延缓腰椎间盘退变(低剂量和高剂量),在溶剂对照组(dimethyil sulfoxide,DMSO)中观察并没有显著改变;3.根据Western blot和real time PCR结果分析,姜黄素治疗组(低剂量和高剂量)与未给予姜黄素治疗组相比,前者能够减少退变腰椎间盘组织中的NF-κB-p65、TNF-a的表达;4.电镜中观察、Western blot和real time PCR结果分析,姜黄素低剂量组和高剂量组之间没有差异。结论高剂量的姜黄素可有效抑制腰椎间盘的退变程度、减少胶原纤维的损伤,并可抑制退变的椎间盘中NF-ΚB-p65活性、降低TNF-alpha的表达水平。
[Abstract]:Objective To observe the effect of curcumin on the expression of nuclear factor -kappa B-p65 (NF- kappa B-p65) and tumor necrosis factor -alpha (TNF-a) in the degenerative nucleus pulposus of rats and to explore the mechanism of delaying the process of degeneration of intervertebral disc. Methods to establish the degenerative change model of the lumbar intervertebral disc in Sprague-Dawley (SD) rats and give curcumin. Treatment, ultrastructural changes of ultrastructure to evaluate the degenerative changes of lumbar intervertebral disc in SD rats and the expression of NF- kappa B-p65 and TNF-a in the degenerative intervertebral disc respectively. Results 1. the lumbar disc degeneration model was successfully established by lumbar nuclear magnetic resonance (MRI) and histomorphology, and 2. through MRI and ultramicro results. It was found that the curcumin treatment group could significantly delay the lumbar intervertebral disc degeneration (low dose and Gao Jiliang), and no significant changes were observed in the solvent control group (dimethyil sulfoxide, DMSO); 3. according to the results of Western blot and real time PCR, the curcumin treatment group (low dose and high dose) could be compared with the non curcumin treatment group. Reduction of NF- kappa B-p65, TNF-a expression in degenerative lumbar intervertebral disc; 4. electrical mirror observation, Western blot and real time PCR results, no difference between curcumin low dose group and high dose group. Conclusion high dose curcumin can effectively inhibit the degree of degeneration of lumbar intervertebral disc, reduce the damage of collagen fiber, and inhibit the degenerative vertebra. The activity of NF- B-p65 in the pan decreased the expression level of TNF-alpha.
【学位授予单位】：宁夏医科大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：R681.53

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