喉癌与HPV、EBV感染阳性表达的相关性

发布时间：2018-01-16 12:10

本文关键词：喉癌与HPV、EBV感染阳性表达的相关性　出处：《实用医学杂志》2017年13期 　论文类型：期刊论文

【摘要】：目的探讨喉癌与HPV、EBV阳性表达的关系。方法采用核酸分子快速导流杂交基因芯片分型技术(Hybri Max)和实时荧光定量PCR技术对101例喉癌石蜡包埋组织标本进行37种HPV亚型检测和EBV定量检测,以43例声带息肉标本为对照。结果喉癌组HPV阳性率13.86%,对照组HPV阳性率9.3%,HPV在喉癌组和对照组的阳性表达差异无统计学意义(P0.05);喉癌组EBV阳性率63.37%,对照组EBV阳性率13.95%,EBV在喉癌组和对照组的阳性表达差异具有统计学意义(P0.05);通过不同分化程度喉癌组织中EBV阳性率的比较,EBV在高分化型与中分化型喉癌组织中阳性表达差异无统计学意义(P0.05),EBV在高分化型与低分化型、中分化型与低分化型喉癌组织中阳性表达差异具有统计学意义(P0.05);不同性别、年龄、病程的喉癌患者中EBV阳性率差异无统计学意义(P0.05)。结论喉癌的发病与EBV感染密切相关,与HPV可能有关,HPV高危亚型感染在诱发喉癌的过程中有一定作用;喉癌患者的性别、年龄、病程长短与EBV感染无明显关系。本研究将为喉癌的病毒学致病机制及临床上防治喉癌提供一定的依据。
[Abstract]:Objective to investigate the relationship between laryngeal carcinoma and HPV. The relationship of EBV positive expression. Methods the gene typing technique of nucleic acid molecular fast diversion hybridization was used. And real-time fluorescence quantitative PCR technique was used to detect 37 kinds of HPV subtypes and EBV quantitative detection in paraffin embedded tissues of 101 cases of laryngeal carcinoma. Results the positive rate of HPV was 13.86 in laryngeal carcinoma group and 9.3% in control group. There was no significant difference in the positive expression of HPV between the laryngeal carcinoma group and the control group (P 0.05). The positive rate of EBV in laryngeal carcinoma group was 63.37 and the positive rate of EBV in control group was 13.95%. To compare the positive rate of EBV in different differentiated laryngeal carcinoma tissues, there was no significant difference in the expression of EBV between well-differentiated and moderately differentiated laryngeal carcinoma tissues (P 0.05). The positive expression of EBV in highly differentiated and poorly differentiated, moderately differentiated and poorly differentiated laryngeal carcinomas was significantly different (P 0.05). There was no significant difference in EBV positive rate among different sex, age and course of laryngeal cancer patients. Conclusion the incidence of laryngeal carcinoma is closely related to EBV infection and may be related to HPV. High risk subtype infection of HPV may play a role in the induction of laryngeal carcinoma. There was no significant relationship between sex, age, course of disease and EBV infection in patients with laryngeal carcinoma. This study will provide evidence for virological mechanism of laryngeal carcinoma and clinical prevention and treatment of laryngeal cancer.
【作者单位】：宁夏医科大学;第二军医大学附属公利医院耳鼻咽喉科;宁夏医科大学总医院耳鼻咽喉科;第二军医大学附属长征医院耳鼻咽喉科;
【基金】：国家自然科学基金(编号:81602369) 浦东新区卫生系统重点学科群资助项目(编号:PWZxq2014-09)
【分类号】：R739.65
【正文快照】： 喉癌在头颈部恶性肿瘤中较常见,其中鳞状细胞癌占绝大多数,我国喉癌平均发病率约占全身恶性肿瘤的1%~5%,在头颈部恶性肿瘤中居第3位[1]。相关流行病学调查显示近年来喉癌的新发病例呈增长趋势,而且不同地区其发病率存在一定的差异[2]。喉癌的病因复杂,多数学者认为是多种因素

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