MTA-2在喉鳞癌中的表达及意义

发布时间：2018-01-16 15:12

本文关键词：MTA-2在喉鳞癌中的表达及意义　出处：《佳木斯大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的：本研究通过检测MTA-2mRNA和蛋白在喉鳞状细胞癌（LSCC）组织中的表达，，分析MTA-2的表达与各临床病理因素之间的关系及临床意义，也为将来把MTA-2作为早期诊断喉癌的指标之一打下基础，并为以后喉鳞状细胞癌的基因治疗提供基础理论依据。方法：实验对象：收集90例喉鳞状细胞癌石蜡标本，其中40例为配对资料（原发灶与相应的淋巴结转移灶）；另收集20例癌旁5mm以外的正常喉上皮组织作为对照。为了验证免疫组化的结果，我们收集30对喉鳞状细胞癌与癌旁正常喉上皮组织的新鲜标本（对应标记）提取其mRNA和蛋白。采用免疫组化方法喉鳞状细胞癌组织标本中MTA-2的表达，并确定MTA-2与患者的年龄和性别，以及喉鳞状细胞癌分化程度、PTNM分期、淋巴结转移等临床病理因素的关系；并检测MTA-2在原发灶与其相对应淋巴结转移灶中的表达差异。利用RT-PCR和Western-blotting方法检测30对喉鳞状细胞癌与癌旁正常喉上皮组织标本中MTA-2的mRNA和蛋白表达情况，并统计分析验证它们与临床病理因素的相关性。应用SPSS for Windows13.0软件进行数据分析。采用Pearson's Chi-Square检验分析MTA-2的表达与喉鳞癌患者临床病理因素的关系；MTA-2在原发灶与LN转移灶表达的比较采用McNemar法、RT-PCR和Western Blotting结果采用Independent-Samples T检验分析。P＜0.05被认为有统计学差异。结果：（1）MTA-2在喉鳞状细胞癌及癌旁喉上皮组织中的表达：90例喉鳞状细胞癌组织中，MTA-2阳性表达率高达64.4%（58/90），且均表达于癌细胞核内，而在20例相应的癌旁喉上皮组织中MTA-2为阴性表达。（2）MTA-2在喉鳞状细胞癌原发灶与配对的淋巴结转移灶中的表达：MTA-2在淋巴结转移灶中的阳性表达率为85%（34/40），明显高于其配对的原发灶的62.5%。（3）MTA-2mRNA在喉鳞状细胞癌及癌旁正常喉上皮组织中的表达：喉鳞状细胞癌组织中为0.99±0.08，癌旁正常喉上皮组织中为0.38±0.14。（4）MTA-2蛋白量在喉鳞状细胞癌及癌旁正常喉上皮组织中的表达：喉鳞状细胞癌组织中为0.92±0.23，癌旁正常喉上皮组织中为0.34±0.12。（5）MTA2mRNA和蛋白的表达与喉鳞状细胞癌患者临床病理特征的关系：MTA-2在低分化，高分期和有淋巴结转移的病例中表达明显增高，MTA-2的表达与喉癌临床PTNM分期呈正相关，与淋巴结转移正相关，与癌肿瘤的分化程度呈负相关，而与患者的性别差异，年龄大小无显著关系。结论：（1）MTA-2基因在喉鳞状细胞癌组织中呈高表达，并且与喉癌临床PTNM分期呈正相关，与淋巴结转移正相关，与癌肿瘤的分化程度呈负相关，与患者的年龄、性别无明显相关性。（2）MTA-2mRNA和蛋白在喉鳞状细胞癌中表达水平明显高于对照的正常喉粘膜上皮组织，之间的差异具有统计学意义。（3）MTA-2基因可促进喉鳞状细胞癌恶性增殖和转移，可作为喉鳞癌早期诊断的又一个重要标志物及基因治疗靶点。
[Abstract]:Objective: to investigate the expression of MTA-2mRNA and protein in laryngeal squamous cell carcinoma (LSCC). To analyze the relationship between the expression of MTA-2 and the clinicopathological factors and its clinical significance, and to lay a foundation for the early diagnosis of laryngeal carcinoma by MTA-2 in the future. It also provides basic theoretical basis for gene therapy of laryngeal squamous cell carcinoma. Methods: ninety paraffin specimens of laryngeal squamous cell carcinoma were collected, 40 of which were matched (primary tumor and corresponding lymph node metastasis). Another 20 cases of normal laryngeal epithelium beyond 5 mm adjacent to carcinoma were collected as control group to verify the immunohistochemical results. MRNA and protein were extracted from 30 pairs of fresh specimens of laryngeal squamous cell carcinoma (LSCC) and adjacent normal laryngeal epithelium. The expression of MTA-2 in laryngeal squamous cell carcinoma (LSCC) tissues was determined by immunohistochemical method. The age and sex of the patients and the differentiation degree of LSCC were determined. Relationship of clinicopathological factors such as lymph node metastasis; The expression of MTA-2 was detected in the primary tumor and its corresponding lymph node metastasis. The expression of MTA-2 mRNA and protein in 30 pairs of laryngeal squamous cell carcinoma (LSCC) and adjacent normal laryngeal epithelial tissues were detected by RT-PCR and Western-blotting methods. The correlation between them and clinicopathological factors was verified by statistical analysis. Data analysis using SPSS for Windows13.0 software. Pearson's. The relationship between the expression of MTA-2 and clinicopathological factors in patients with laryngeal squamous cell carcinoma was analyzed by Chi-Square test. McNemar method was used to compare the expression of MTA-2 in primary tumor and LN metastasis. RT-PCR and Western Blotting results using Independent-Samples. T test analysis. P < 0.05 was considered to have statistical difference. Results the positive expression rate of MTA-2 in laryngeal squamous cell carcinoma and adjacent laryngeal epithelium was as high as 64.4% / 90 in 90 cases of laryngeal squamous cell carcinoma (LSCC). All of them were expressed in the nuclei of cancer cells. The expression of MTA-2 was negative in the corresponding laryngeal epithelium of 20 cases. The positive expression rate of MTA-2 in primary and paired lymph node metastases of laryngeal squamous cell carcinoma was 85 / 34 / 40). Significantly higher than 62.5% of its matched primary foci. Expression of MTA-2mRNA in laryngeal squamous cell carcinoma and adjacent normal laryngeal epithelium: 0.99 卤0.08 in laryngeal squamous cell carcinoma. It was 0.38 卤0.14. 4 in normal laryngeal epithelium adjacent to cancer. Expression of MTA-2 protein in laryngeal squamous cell carcinoma and adjacent normal laryngeal epithelium: 0.92 卤0.23 in laryngeal squamous cell carcinoma. The relationship between the expression of MTA2 mRNA and protein in the adjacent normal laryngeal epithelium and the clinicopathological features of laryngeal squamous cell carcinoma (LSCC): MTA-2 is poorly differentiated. The expression of MTA-2 in patients with high staging and lymph node metastasis was positively correlated with the clinical PTNM stage of laryngeal carcinoma, positively correlated with lymph node metastasis, and negatively correlated with the degree of tumor differentiation. However, there was no significant relationship between gender difference and age. Conclusion the expression of MTA-2 gene in laryngeal squamous cell carcinoma is positively correlated with the clinical PTNM stage of laryngeal carcinoma and lymph node metastasis. There was a negative correlation with the degree of differentiation of cancer tumor, and a negative correlation with the age of the patients. The expression of MTA-2 mRNA and protein in laryngeal squamous cell carcinoma was significantly higher than that in normal laryngeal mucosal epithelium. MTA-2 gene can promote the malignant proliferation and metastasis of laryngeal squamous cell carcinoma and can be used as an important marker and gene therapy target for early diagnosis of laryngeal squamous cell carcinoma.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

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