CBX7在喉鳞状细胞癌组织中的表达及意义

发布时间：2018-01-17 13:33

本文关键词：CBX7在喉鳞状细胞癌组织中的表达及意义　出处：《山东医药》2017年06期 　论文类型：期刊论文

【摘要】：目的观察喉鳞状细胞癌(LSCC)组织中CBX7 mRNA和蛋白的表达,探讨其在LSCC发生发展中的作用。方法收集手术切除的LSCC组织及对应的癌旁正常黏膜组织60例,采用real-time PCR法检测CBX7 mRNA表达,Western blot法检测CBX7蛋白表达,分析CBX7 mRNA和蛋白表达与LSCC患者临床病理特征的关系。结果LSCC组织和癌旁正常黏膜组织中CBX7 mRNA的相对表达量分别为6.79±2.36、1.04±0.51,LSCC组织和癌旁正常黏膜组织中CBX7蛋白的相对表达量分别为0.51±0.16、0.15±0.08,LSCC组织中CBX7 mRNA和蛋白的表达均高于癌旁正常黏膜组织(P均0.01)。临床分期高、合并颈部淋巴结转移、组织分化程度低者CBX7 mRNA和蛋白表达高于临床分期低、无颈部淋巴结转移、组织分化程度高者(P均0.01)。结论 LSCC组织中CBX7表达升高,CBX7参与了肿瘤形成和转移过程,可能成为LSCC治疗的新靶点和预后评价指标。
[Abstract]:Objective to investigate the expression of CBX7 mRNA and protein in laryngeal squamous cell carcinoma (LSCC). To investigate its role in the development of LSCC. Methods 60 cases of LSCC tissues and adjacent normal mucosa tissues were collected. CBX7 mRNA expression was detected by real-time PCR method and CBX7 protein expression was detected by Western blot assay. To analyze the relationship between the expression of CBX7 mRNA and protein and the clinicopathological characteristics of LSCC patients. Results CBX7 was found in LSCC tissues and adjacent normal mucosa. The relative expression of mRNA was 6.79 卤2.36, respectively. The relative expression of CBX7 protein was 0.51 卤0.16 卤0.15 卤0.08 in LSCC and adjacent normal mucosa, respectively. The expression of CBX7 mRNA and protein in LSCC was higher than that in adjacent normal mucosa (P 0.01). The expression of CBX7 mRNA and protein in patients with low tissue differentiation was higher than that in clinical stage, and there was no cervical lymph node metastasis. Conclusion the increased expression of CBX7 in LSCC may play an important role in the process of tumor formation and metastasis. It may be a new target and prognostic index for LSCC therapy.
【作者单位】：中国医科大学附属盛京医院;
【分类号】：R739.65
【正文快照】： 喉鳞状细胞癌(LSCC)是喉恶性肿瘤的主要类型,占所有喉癌患者的95%[1]。目前喉癌的治疗以手术为中心的综合治疗为主,但中晚期喉癌由于复发及转移的原因致死率仍较高,而且使多数患者失去语言功能[2,3]。T3、T4期喉癌5年生存率只达到50%,因此,对于喉癌分子生物学机制的研究十分重

【相似文献】