噪声对豚鼠耳蜗基底膜乙酰化组蛋白H2B表达的影响

发布时间：2018-01-17 14:00

本文关键词：噪声对豚鼠耳蜗基底膜乙酰化组蛋白H2B表达的影响　出处：《听力学及言语疾病杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的观察噪声性聋豚鼠耳蜗基底膜毛细胞乙酰化组蛋白H2B表达水平的变化,探讨组蛋白乙酰化与噪声性听力损失的相关性。方法成年雄性白色红目豚鼠60只随机分为噪声组和对照组,两组豚鼠分别进行听性脑干反应(ABR)检测后,噪声组给予高强度窄带噪声(122dB SPL,3小时)暴露,对照组不予任何处理;噪声组于噪声暴露后1小时、3天、7天、14天和21天分别行ABR检测;并以免疫荧光染色和Western blot方法检测两组豚鼠耳蜗基底膜听觉细胞中乙酰化组蛋白H2B的表达水平。结果噪声暴露后1小时、3天、7天、14天、21天噪声组豚鼠ABR各频率反应阈均较噪声暴露前及对照组显著增高,且随时间的延长听力逐渐恢复,14天时趋于稳定,达到永久性阈移。免疫荧光染色显示在噪声组豚鼠耳蜗内外毛细胞及Hensen's细胞中乙酰化组蛋白H2B荧光强度较正常对照组减弱,Western blot结果显示噪声组豚鼠耳蜗基底膜中乙酰化组蛋白H2B的表达(H2BAcK5/β-actin比值为0.310 2±0.083 9)较对照组(0.617 9±0.126)明显下调,两组差异有统计学意义(P0.01)。结论噪声可导致豚鼠内耳感觉细胞乙酰化组蛋白H2B表达水平下降,乙酰化组蛋白失衡有可能参与了噪声性聋的发生。
[Abstract]:Objective to observe the changes of acetylated histone (H2B) expression in the hair cells of basal membrane of cochlea of noise induced deafness guinea pig. To investigate the correlation between histone acetylation and noise-induced hearing loss 60 adult male white red guinea pigs were randomly divided into noise group and control group. After the auditory brainstem response (ABR) was detected in the two groups, the noise group was exposed to high intensity narrowband noise of 122 dB SPLN for 3 hours, while the control group was not treated with any treatment. In the noise group, ABR was detected at 1 hour, 3 days, 7 days, 14 days and 21 days, respectively. The expression of acetylated histone H2B in auditory cells of basal membrane of guinea pig cochlea was detected by immunofluorescence staining and Western blot. The frequency response thresholds of ABR in the noise group were significantly higher than those in the control group and before noise exposure, and tended to be stable with the prolongation of the time. The fluorescence intensity of acetylated histone H2B in the inner and outer hair cells of cochlea and Hensen's cells in the noise group was lower than that in the normal control group. The results of Western blot showed the expression of acetylated histone H2B in the basal membrane of guinea pig cochlea with noise (. The ratio of H2BAcK5 / 尾 -actin was 0.310 2 卤0.083 9) significantly lower than that of control group (0.617 9 卤0.126). Conclusion noise can decrease the expression of acetylated histone H2B in the inner ear of guinea pig. Acetylated histone imbalance may be involved in noise-induced deafness.
【作者单位】：首都医科大学附属复兴医院耳鼻喉头颈外科;首都医科大学附属北京友谊医院耳鼻喉头颈外科;
【基金】：国家自然科学基金资助(81300831)
【分类号】：R764.433
【正文快照】： 网络出版地址:http://kns.cnki.net/kcms/detail/42.1391.R.20170705.1715.002.html随着社会工业化和现代化的发展,噪声性聋的发病人群也越来越多,目前全世界约有15%的人口存在不同程度和类型的听力损失[1],噪声已成为引起感音神经性聋的第二大病因,仅次于老年性聋[2]。目前,

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