实验性外斜视猫内直肌M-cadherin和CNTF表达

发布时间：2018-01-18 03:09

本文关键词：实验性外斜视猫内直肌M-cadherin和CNTF表达　出处：《青岛大学》2012年硕士论文　论文类型：学位论文

更多相关文章： 外斜视猫模型 内直肌 M-cadherin CNTF

【摘要】：目的观察外斜视猫模型中内直肌M-钙粘蛋白(M-cadherin)和睫状神经营养因子(CNTF)的表达变化以及与戴镜时间的关系,探讨共同性外斜视的发病机制。方法选择处于2-4周龄的幼猫15只,雌雄不限,随机分为实验组(9只)和对照组(6只),实验组佩戴底向外三棱镜眼镜制作外斜视模型,根据戴镜时间分为A、B、C三组,每组3只；对照组也相应分为3组,每组2只。分别于戴镜后1个月、2个月、3个月取内直肌,免疫组化SP法测量M-cadherin和CNTF的光密度值,统计分析实验组与对照组以及各实验组间的表达差异。结果实验组外斜视猫内直肌M-cadherin平均光密度值均较对照组低,A组和对照A组平均光密度值分别为0.0291±0.0073和0.0386±0.0074,差异无统计学意义(P0.05)；B组和对照B组的平均光密度值为0.0252±0.0046和0.0379±0.0072,C组和对照C组的平均光密度值为0.0206±0.0035和0.0386±0.0057,两组差异均有统计学意义(P0.05)；经方差分析,实验组三组组间差异有统计学意义(P0.05),而对照组组间无统计学意义(P0.05)。实验组CNTF的平均光密度值较对照组低,A、B、C三组分别为0.364±0.0170、0.328±0.0180和0.277±0.0207,对照组分别为0.383±0.0172、0.384±0.0117和0.380±0.0273,除A组无统计学意义外(P0.05),B、C两组差异均有统计学意义(P0.05)；A、B、C三组组间差异也有统计学意义(p0.05),而对照组组间无统计学意义(P0.05)。结论外斜视模型内直肌中(?)-cadherin和(?)CNTF表达降低,可能与共同性外斜视的发生有关。外斜视模型内直肌中CNTF的表达随着戴镜时间的延长逐渐降低,可能是共同性外斜视眼外肌失神经病理改变逐渐加重的因素。
[Abstract]:Objective to observe the expression of M-cadherin (M-cadherin) and ciliary neurotrophic factor (CNTF) in rectus rectus muscle and the relationship between the expression of M-cadherin and the time of endoscope in exotropia cat model. To explore the pathogenesis of concomitant exotropia. Methods Fifteen kittens aged 2-4 weeks were randomly divided into experimental group (n = 9) and control group (n = 6). According to the time of wearing glasses, they were divided into three groups: group A, group B (n = 3) and group C (n = 3). The control group was also divided into 3 groups, 2 rats in each group, and the optical density of M-cadherin and CNTF were measured by immunohistochemical SP method after one month, two months and three months after wearing the mirror. The difference of expression between the experimental group and the control group was analyzed statistically. Results the average optical density of M-cadherin in the experimental group was lower than that in the control group. The average optical density of group A and control group A were 0.0291 卤0.0073 and 0.0386 卤0.0074, respectively. There was no significant difference between group A and group A (P 0.05). The average optical density of group B and control group B were 0.0252 卤0.0046 and 0.0379 卤0.0072 respectively. The average optical density of group C and C was 0.0206 卤0.0035 and 0.0386 卤0.0057, respectively. The analysis of variance showed that the difference among the three groups in the experimental group was statistically significant, but there was no significant difference between the control group and the control group. The average optical density of CNTF in the experimental group was lower than that in the control group. The values of the three groups were 0.364 卤0.0170, 0.328 卤0.0180 and 0.277 卤0.0207, respectively, and those of the control group were 0.383 卤0.0172, respectively. 0.384 卤0.0117 and 0.380 卤0.0273, with the exception of group A, there was no statistical difference between group A and group C (P 0.05). There was also a significant difference among the three groups (P 0.05), but there was no significant difference between the control group (P 0.05) and the control group (P 0.05). Conclusion the medial rectus muscle of the exotropia model. Cudherin and Con? The decreased expression of CNTF may be related to the occurrence of concomitant exotropia. The expression of CNTF in rectus muscle of exotropia model decreases gradually with the prolongation of the time of wearing glasses. It is possible that the pathological changes of the denervated extraocular muscles in concomitant exotropia are gradually aggravated.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.41

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