Brg1基因与喉癌发生相关性研究

发布时间：2018-01-19 13:29

本文关键词： Brg1 喉癌单核苷酸多态性免疫组织化学　出处：《吉林大学》2012年硕士论文　论文类型：学位论文

【摘要】：Brg1基因是进化上高度保守的SWI/SNF染色质重塑复合物的核心亚基,是近年来发现的一个肿瘤抑制因子。Brg1在调节细胞周期、发育分化、DNA修复和肿瘤发生等进程中发挥着重要的作用。而且，Brg1能够与RB、p53、CD44、BRCA-1等细胞因子相互作用，激活或抑制转录。研究发现，Brg1在肺癌、乳腺癌、前列腺癌、胰腺癌等细胞系中都有不同程度的突变或缺失。本实验主要采用PCR-RFLP和免疫组织化学的方法，探讨Brg1基因与喉癌发生的相关性。通过对98例喉癌患者和100例正常对照组的血样研究，发现Brg1的rs60448955、rs12609589、rs75904516位点的等位基因频率在喉癌组和对照组之间没有差异（P＞0.05），与喉癌的发生可能没有相关性；rs73013167位点的G、C等位基因频率在喉癌组和对照组之间有显著差异（P＜0.05），说明该位点可能是喉癌发生的易感位点。为了进一步研究Brg1在喉癌组织中的表达情况，采用了免疫组织化学的方法，对20例喉癌组织和10例癌旁组织染色分析，发现Brg1在癌旁正常组织中呈阳性表达，阳性率为90%；在喉癌组织中表达明显降低，阳性率为35%，两组间阳性率有显著差异（P＜0.05）。并且，在高、中、低分化的喉癌组织中，Brg1的阳性表达逐渐降低；有淋巴转移组织Brg1的阳性表达也要低于没有淋巴转移的组织，说明Brg1与喉癌的发生、发展有关，在维持喉粘膜的正常形态和功能上有意义。国内外鲜见关于Brg1与喉癌的报道，本实验从分子生物学和组织学两方面论证了Brg1与喉癌的发生具有相关性，，为进一步探讨喉癌发生的分子机制奠定了基础，为临床诊断和治疗提供了新的思路。
[Abstract]:Brg1 gene is the core subunit of the highly conserved SWI/SNF chromatin remodeling complex. It is a tumor suppressor. Brg1 regulates cell cycle and differentiation. DNA repair and tumorigenesis play an important role in the process, and Brg1 can interact with cytokines such as RBP53, CD4, BRCA-1 and so on. Activates or suppresses transcription. Studies have found that Brg1 is present in lung cancer, breast cancer, and prostate cancer. Pancreatic cancer and other cell lines have varying degrees of mutation or deletion. PCR-RFLP and immunohistochemical methods were used in this study. To investigate the relationship between Brg1 gene and laryngeal carcinoma, the rs60448955 of Brg1 was found in 98 patients with laryngeal carcinoma and 100 normal controls. The allele frequency of rs12609589 rs75904516 was not different between the laryngeal cancer group and the control group (P > 0.05), and there may be no correlation between the allele frequency of rs12609589 or rs75904516 and the occurrence of laryngeal carcinoma. There was significant difference in allele frequency of rs73013167 between laryngeal carcinoma group and control group (P < 0.05). In order to further study the expression of Brg1 in laryngeal carcinoma tissues, immunohistochemical method was used. 20 cases of laryngeal carcinoma and 10 cases of paracancerous tissues were analyzed. The positive expression of Brg1 was found in normal tissues of adjacent larynx, and the positive rate was 90%. The positive rate was 35% in laryngeal carcinoma tissues, and there was significant difference between the two groups (P < 0.05). The positive expression of Brg1 decreased gradually. The positive expression of Brg1 in lymphatic metastasis tissues was also lower than that in non-lymphatic metastasis tissues, indicating that Brg1 was related to the occurrence and development of laryngeal carcinoma. It is significant to maintain the normal morphology and function of laryngeal mucosa. There are few reports about Brg1 and laryngeal carcinoma at home and abroad. This study demonstrated the correlation between Brg1 and laryngeal carcinogenesis from two aspects of molecular biology and histology, which laid a foundation for further study on the molecular mechanism of laryngeal carcinogenesis. It provides a new idea for clinical diagnosis and treatment.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

【相似文献】