实验性外斜视猫内直肌IGF-1、Anexin A1的表达变化

发布时间：2018-01-22 18:01

本文关键词： 外斜视猫眼外肌胰岛素样生长因子Ⅰ 膜联蛋白A1　出处：《青岛大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的观察斜视猫模型弱侧眼外肌中IGF-I及Annexin A1的表达变化,探讨IGF-I、Annexin A1与共同性外斜视发生、发展的关系。方法3-4周龄幼猫15只,随机分为实验组(9只)和对照组(6只)。实验组猫均佩戴猫类动物用三棱镜眼镜。角膜映光法测量斜视度数,确定斜视模型建立。根据实验猫佩戴三棱镜眼镜持续时间又分为A、B、C三组,其中A组戴镜1个月,B组戴镜2个月,C组戴镜3个月。分别于佩戴三棱镜眼镜1、2、3个月后取实验组及同龄对照组猫内直肌,HE染色观察内直肌形态学表现,免疫组织化学染色,测(?)IGF-1、AnnexinA1在眼外肌中的平均光密度值。t检验统计比较各实验组及其同龄对照组组间平均光密度值,单因素方差分析比较实验组、对照组组间IGF-1、Annexin A1蛋白表达差异。结果①实验组猫IGF一1的表达较对照组降低,平均光密度值分别为0.300±0.012、0.265±0.038及0.195±0.028。与同龄对照组比较均有统计学差异(P0.05)。②实验组猫AnnexinAl的表达显著低于同龄对照组,平均光密度值分别为0.235±0.015、0.185±0.024及0.114±0.017。与对照组比较均有显著统计学差异(P0.01)。③实验组内直肌IGF-1、AnnexinA1平均光密度值随戴镜时间延长逐渐降低,组间比较有统计学差异(P0.05)。④各对照组内直肌IGF-1、 AnnexinA1表达无明显变化,组间比较无统计学差异(P0.05)。结论实验性外斜视猫弱侧眼外肌中IGF-I、Annexin A1表达的降低,可能参与共同性外斜视的发病。实验性外斜视猫弱侧眼外肌中IGF一1、Annexin A1的表达随戴镜时间延长逐渐降低,可能与共同性外斜视的发展相关。佩戴三棱镜眼镜能成功诱导共同性外斜视,可作为共同性斜视动物模型的制备方法。
[Abstract]:Objective to observe the expression of IGF-I and Annexin A1 in the weak lateral extraocular muscle of strabismus cat model, and to explore the occurrence of IGF-I Annexin A1 and concomitant exotropia. The relationship between development Methods Fifteen kittens aged 3-4 weeks were randomly divided into two groups: experimental group (n = 9) and control group (n = 6). All cats in the experimental group wore prism glasses and the squint degree was measured by corneal enantiometry. According to the duration of prism glasses, the cats were divided into three groups: group A wearing glasses for 1 month and group B wearing glasses for 2 months. Group C wore glasses for 3 months. After 3 months of wearing prism glasses, the experimental group and the control group were stained with HE staining to observe the morphology of the rectus muscle. The mean optical density of IGF-1 Annexin A1 in the extraocular muscle was compared with the average optical density of the experimental group and the control group of the same age. The single factor variance analysis was used to compare the experimental group. The expression of IGF-1 Annexin A1 protein was different between the control group and control group. Results 1 the expression of IGF-1 in the experimental group was lower than that in the control group, and the average optical density was 0. 300 卤0. 012, respectively. 0.265 卤0.038 and 0.195 卤0.028 respectively. 2 the expression of AnnexinAl in the experimental group was significantly lower than that in the control group. The average optical density was 0.235 卤0.015. 0.185 卤0.024 and 0.114 卤0.017.The difference was significant compared with the control group (P 0.01). 3. IGF-1 of the medial rectus muscle in the experimental group was significantly different from that in the control group. The mean optical density of AnnexinA1 decreased gradually with the prolongation of the time of wearing the lens. There was a significant difference between the two groups (P 0.05). 4. The IGF-1 of rectus muscle in each control group was significantly lower than that in the control group. There was no significant change in AnnexinA1 expression and there was no statistical difference between the two groups (P 0.05). Conclusion the expression of IGF-I and Annexin A1 in the weak lateral extraocular muscles of experimental exotropia cats is decreased. The expression of IGF 1 and Annexin A1 in the weak lateral extraocular muscles of experimental exotropia cats decreased with the prolongation of the time of wear. It may be related to the development of concomitant exotropia. Wearing prism glasses can induce concomitant exotropia successfully and can be used as a preparation method of concomitant exotropia animal model.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.41

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