微小RNA-34a在喉鳞状细胞癌的功能研究及临床意义

发布时间：2018-01-24 07:05

本文关键词： 微小RNA-34a 生存素喉鳞状细胞癌抑癌因子　出处：《宁波大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的生存素（Survivin）具有强大的抗凋亡作用，被认为是肿瘤基因治疗的理想靶点。微小RNA-34a (miR-34a)通过负向调控某些肿瘤中特定基因的表达而发挥抑癌因子的作用。因此，本研究旨在探讨miR-34a和Survivin在喉鳞状细胞癌(LSCC)中相互作用的关系。方法 1.采用脂质体把miR-34a类似物导入人喉鳞状细胞癌细胞系Hep-2细胞中，以提高Hep-2细胞中miR-34a的表达水平。四甲基偶氮唑蓝（MTT）法检测Hep-2细胞的增殖情况，，蛋白免疫印迹（Western blot）法检测miR-34a对Survivin蛋白表达的影响，流式细胞术检测细胞周期的变化。 2.实时定量聚合酶链反应（PCR）检测喉鳞状细胞癌中miR-34a及Survivin信使RNA的表达水平，并分析其表达水平与喉鳞状细胞癌的病理分化，淋巴结转移，临床分期及生存率的关系。结果 1. miR-34a可以明显的抑制Hep-2细胞的增殖，并且可以在不影响Survivin信使RNA的表达水平的情况下明显下调Survivin蛋白的表达。此外，miR-34a使Hep-2细胞的细胞周期阻滞在G0/G1期。 2.喉鳞状细胞癌组织标本结果表明，miR-34a在肿瘤组织中是低表达的，而Survivin是高表达的。miR-34a的表达水平与喉鳞状细胞癌患者的病理分化，淋巴结转移，临床分期负相关，与生存率正相关。结论 miR-34a可能通过靶向Survivin与喉鳞状细胞癌的发生密切相关，在喉鳞状细胞癌的发生发展中起着重要作用。
[Abstract]:Purpose Survivin (survivin) has a strong anti-apoptotic effect. Small RNA-34a miR-34a) acts as a tumor suppressor by negatively regulating the expression of specific genes in certain tumors. The purpose of this study was to investigate the interaction between miR-34a and Survivin in laryngeal squamous cell carcinoma (LSCC). Method 1. MiR-34a analogue was introduced into human laryngeal squamous cell carcinoma cell line Hep-2 cells by liposome. In order to increase the expression of miR-34a in Hep-2 cells, the proliferation of Hep-2 cells was detected by MTT assay. The effect of miR-34a on the expression of Survivin protein was detected by Western blotting, and the cell cycle was detected by flow cytometry. 2.Real-time quantitative polymerase chain reaction (PCR) was used to detect the expression of miR-34a and Survivin messenger RNA in laryngeal squamous cell carcinoma. The relationship between the expression level and pathological differentiation, lymph node metastasis, clinical stage and survival rate of laryngeal squamous cell carcinoma (LSCC) was analyzed. Results 1. MiR-34a could significantly inhibit the proliferation of Hep-2 cells. Moreover, the expression of Survivin protein could be significantly down-regulated without affecting the expression level of Survivin messenger RNA. MiR-34a blocked the cell cycle of Hep-2 cells in G _ 0 / G _ 1 phase. 2. The expression of miR-34a in laryngeal squamous cell carcinoma tissues was low. However, the expression of .miR-34a in Survivin was negatively correlated with pathological differentiation, lymph node metastasis, clinical stage and survival rate in laryngeal squamous cell carcinoma. Conclusion MiR-34a may be closely related to laryngeal squamous cell carcinoma by targeting Survivin, and play an important role in the development of laryngeal squamous cell carcinoma.
【学位授予单位】：宁波大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.65

【共引文献】