糖皮质激素对大鼠鼻黏膜AR及SP表达的影响

发布时间：2018-01-31 02:35

本文关键词： AR 大鼠过敏 SP 炎症细胞　出处：《南华大学》2016年硕士论文　论文类型：学位论文

【摘要】：目的:变应性鼻炎(allergic rhinitis,AR)是鼻黏膜过敏性炎症,AR在临床上属常见病多发病。因此,本研究探讨糖皮质激素对大鼠鼻黏膜AR及SP表达的影响,观察鼻黏膜组织嗜酸性粒细胞及肥大细胞浸润。方法:本实验选用30只大鼠,采用随机方法,分为糖皮质激素喷鼻治疗AR实验组及对照组。对照组包括2组,分别为AR对照组及空白对照组,每组10只。AR实验组及AR对照组采用卵清蛋白腹腔注射基础致敏,加双侧鼻腔局部滴卵清蛋白激发,建立大鼠AR模型,空白对照组用等量生理盐水同法处理。末次局部激发后,观察并记录大鼠症状及行为变化。AR模型成功建立后,AR实验组用糖皮质激素布地奈德鼻喷雾剂喷鼻治疗3周,对照组用等量生理盐水鼻喷雾剂喷鼻处理,末次喷鼻治疗后,再次观察并记录大鼠症状及行为变化,水合氯醛麻醉处死3组大鼠,切取大鼠鼻黏膜组织,经甲醛固定、脱水、石蜡、切片,分别施行HE染色、甲苯胺蓝染色及免疫组化染色,显微镜下观察鼻黏膜组织中嗜酸粒细胞、肥大细胞的浸润及SP的表达。结果:采用卵清蛋白腹腔注射基础致敏,加鼻腔局部滴卵清蛋白激发,成功建立了大鼠AR模型;AR实验组施行糖皮质激素布地奈德鼻喷雾剂治疗后,AR实验组大鼠搔鼻、喷嚏、流涕症状较生理盐水鼻喷雾剂喷鼻治疗的AR对照组明显减少,而与空白对照组相似;AR对照组,仅用生理盐水处理,大鼠则有明显的搔鼻、喷嚏、流涕等AR症状表现;经3周治疗,各组大鼠采用水合氯醛麻醉处死,取大鼠鼻黏膜组织进行病理学检查,AR实验组鼻黏膜组织中嗜酸性粒细胞及肥大细胞浸润明显低于生理盐水鼻喷雾剂喷鼻处理的AR对照组,差异具有统计学意义(P0.05),而与空白对照组相似,差异无统计学意义(P0.05);糖皮质激素布地奈德鼻喷雾剂喷鼻治疗的AR实验组SP表达量低于AR对照组,差异具有统计学意义(P0.05),而与空白对照组相似,差异无统计学意义(P0.05)。结论:1.卵清蛋白腹腔注射,加鼻腔局部滴入,可建立大鼠AR模型。2.糖皮质激素布地奈德鼻喷雾剂可有效缓解AR过敏症状,减轻嗜酸性粒细胞及肥大细胞的浸润,减少SP的释放。
[Abstract]:Objective: allergic rhinitis ARA is a common disease of allergic inflammation of nasal mucosa. This study was to investigate the effect of glucocorticoid on AR and SP expression in nasal mucosa of rats, and to observe the infiltration of eosinophils and mast cells in nasal mucosa. Divided into glucocorticoid nasal spray treatment AR experimental group and control group, the control group includes two groups, AR control group and blank control group. Ten rats in each group were sensitized by intraperitoneal injection of ovalbumin (ovalbumin) and stimulated by local ovalbumin dropping in bilateral nasal cavity to establish AR model of rats. The blank control group was treated with the same amount of normal saline. After the last local excitation, the symptoms and behavioral changes of rats were observed and recorded. The AR model was established successfully. The AR experimental group was treated with glucocorticoid budesonide nasal spray for 3 weeks, the control group was treated with the same amount of saline nasal spray. After the last nasal spray treatment, the symptoms and behavior of the rats were observed and recorded again. Three groups of rats were anesthetized by chloral hydrate. The nasal mucosa of the rats was cut off, fixed by formaldehyde, dehydrated, paraffin, section, respectively, HE staining, toluidine blue staining and immunohistochemical staining. The infiltration of eosinophils and mast cells and the expression of SP in nasal mucosa were observed under microscope. Rat AR model was established successfully. After budesonide nasal spray treatment, the symptoms of nasal scratching, sneezing and runny in AR experimental group were significantly lower than those in AR control group treated with normal saline nasal spray. But similar to the blank control group. In the AR control group, the rats were treated with normal saline only, but the rats had obvious symptoms such as scratching nose, sneezing and running snot. After 3 weeks of treatment, the rats in each group were killed by chloral hydrate anesthesia, and the nasal mucosa of the rats were taken for pathological examination. The infiltration of eosinophilic granulocytes and mast cells in nasal mucosa in AR group was significantly lower than that in AR control group treated with normal saline nasal spray (P 0.05). But similar to the blank control group, the difference was not statistically significant (P 0.05). The SP expression in AR experimental group treated with budesonide nasal spray was lower than that in AR control group, the difference was statistically significant (P 0.05), but similar to that in blank control group. Conclusion: 1. Ovalbumin was injected intraperitoneally with nasal drip. Budesonide nasal spray of glucocorticoid can effectively relieve allergic symptoms of AR, reduce infiltration of eosinophils and mast cells, and reduce SP release.
【学位授予单位】：南华大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R765.21

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