过敏性鼻炎合并哮喘患者血液嗜酸性粒细胞富集群中IL-18、IL-18BP及IL-18R的表达

发布时间：2018-01-31 03:51

  本文关键词： 过敏性鼻炎合并哮喘 嗜酸性粒细胞 IL- IL-BP IL-R　出处：《郑州大学学报(医学版)》2017年05期 　论文类型：期刊论文

【摘要】：目的:检测过敏性鼻炎合并哮喘(AR~+AS)患者血液嗜酸性粒细胞富集群中IL-18、IL-18BP和IL-18R的表达。方法:采集13例AR~+AS患者和14名健康人(正常对照组)外周静脉血,用流式细胞术检测静息状态下和过敏原(大籽蒿花粉、尘螨和梧桐花粉过敏原粗提液)刺激后嗜酸性粒细胞富集群中IL-18~+、IL-18BP~+和IL-18R~+细胞比例及平均荧光强度(MFI),分析其相关性。结果:与正常对照组相比,静息状态下AR~+AS患者嗜酸性粒细胞富集群中IL-18~+和IL-18BP~+细胞比例分别升高了5.821倍(P=0.001)和44.1%(P=0.048),IL-18~+细胞MFI增强了29.1%(P=0.001),IL-18BP~+细胞MFI降低了32.3%(P0.001),而IL-18R~+细胞比例及MFI无明显变化(P0.05)。过敏原刺激对AR~+AS患者嗜酸性粒细胞富集群中IL-18、IL-18BP及IL-18R的表达无影响(P0.05)。AR~+AS患者血液嗜酸性粒细胞富集群中IL-18~+和IL-18BP~+细胞比例呈中度相关(r=0.622,P0.001)。结论:嗜酸性粒细胞源的IL-18和IL-18BP可能在AR~+AS中起重要作用。
[Abstract]:Objective: to detect the allergic rhinitis and asthma (AR~+AS) patients with blood eosinophil eosinophil accumulation in IL-18 group, the expression of IL-18BP and IL-18R. Methods: collected 13 cases of AR~+AS patients and 14 healthy people (normal control group) peripheral venous blood with resting cells was detected by flow cytometry and allergens (large Artemisia pollen, dust mites and pollen allergen extract Indus) stimulated eosinophils eosinophil accumulation in IL-18~+ group, IL-18BP~+ and IL-18R~+ cell proportion and mean fluorescence intensity (MFI), and the correlation was analyzed. Results: compared with normal control group, resting state AR~+AS patients with eosinophilic and IL-18~+ enrichment group the percentage of IL-18BP~+ cells were increased by 5.821 times (P=0.001) and 44.1% (P=0.048), IL-18~+ cell MFI was increased by 29.1% (P=0.001), IL-18BP~+ MFI cells decreased by 32.3% (P0.001), and IL-18R~+ cells than cases and no significant change in MFI (P0.05) allergen. Stimulation of the AR~+AS patients with eosinophilic enrichment group IL-18, had no effect on the expression of IL-18BP and IL-18R (P0.05) IL-18~+ from the blood of patients with.AR~+AS and IL-18BP~+ cell ratio was eosinophil enriched group (r=0.622, P0.001) moderate correlation. Conclusion: eosinophil derived IL-18 and IL-18BP may play an important role in AR~+AS.

【作者单位】： 锦州医科大学附属第一医院变态反应与临床免疫研究中心;苏州市相城人民医院中心实验室;
【基金】：国家自然科学基金资助项目81471592;81472016
【分类号】：R562.25;R765.21
【正文快照】： 州21510018R+细胞比例及平均荧光强度(MFI),分析其相关性。结果:与正常对照组相比,静息状态下AR+AS患者嗜酸性粒细胞富集群中IL-18+和IL-18BP+细胞比例分别升高了5.821倍(P=0.001)和44.1%(P=0.048),IL-18+细胞MFI增强了29.1%(P=0.001),IL-18BP+细胞MFI降低了32.3%(P0.001),而，

本文编号：1478116