BRMS1基因在不同转移习性的人鼻咽癌细胞株中表达水平的研究

发布时间：2018-02-15 15:10

本文关键词： 鼻咽肿瘤鼻咽癌转移抑制基因 BRMS1 乳腺癌转移抑制基因1 实时荧光定量PCR 免疫组织化学法 Western blot　出处：《蚌埠医学院》2012年硕士论文　论文类型：学位论文

【摘要】：目的：探讨不同转移潜能的人鼻咽癌细胞株中BRMS1基因在mRNA和蛋白表达水平的差异，并为课题组下一步动物实验提供细胞理论基础。方法:体外培养5株不同组织学类型及不同转移习性的人鼻咽癌细胞株CNE-1、CNE-2Z、SUNE-1、SUNE-1-5-8F、SUNE-1-6-10B。1.应用实时荧光定量PCR法（real-time PCR）检测5种人鼻咽癌细胞株中BRMS1基因mRNA的表达水平；2.免疫组化和Western blot两种方法检测5种人鼻咽癌细胞株中BRMS1蛋白的定性及定量表达水平。结果:1.应用实时荧光定量PCR法检测，发现不同转移习性的人鼻咽癌细胞均扩增出BRMS1mRNA，且随着细胞转移习性增高，BRMS1mRNA表达水平逐渐降低(p0.05)；2.通过Western-blot技术检测了BRMS1蛋白在人鼻咽癌细胞中的表达，结果显示，BRMS1基因随着细胞转移习性增高，蛋白表达水平逐渐降低(p0.05)；3.通过免疫组织化学方法检测了BRMS1在鼻咽癌细胞中的表达，结果表明BRMS1在鼻咽癌细胞膜和/或细胞质中表达。结论:1. BRMS1基因在不同人鼻咽癌细胞株中mRNA水平和蛋白水平均有表达且表达具有一致性，，随着BRMS1基因mRNA水平表达的增高，在蛋白水平表达也呈增高趋势；2.BRMS1基因在mRNA水平随鼻咽癌细胞株转移潜能增高表达量下降；3.BRMSI基因在蛋白水平随鼻咽癌细胞株转移潜能增高表达量下降；4.BRMS1基因在低转移性细胞株中高表达，而在高转移性细胞中低表达，且其表达强度随细胞株的转移潜能的增加而降低，提示了BRMS1可能在鼻咽癌肿瘤细胞转移机制中发挥重要的抑制作用。
[Abstract]:Aim: to investigate the expression of BRMS1 gene in human nasopharyngeal carcinoma (NPC) cell lines with different metastatic potential and to provide a theoretical basis for further animal experiments. Methods: five human nasopharyngeal carcinoma cell lines CNE-1, CNE-2Z, SUNE-1-5-5-8 FT-SUNE-1-6-6-10B.1.The expression of BRMS1 gene mRNA in 5 human nasopharyngeal carcinoma cell lines was detected by real-time PCR method. The expression level of BRMS1 gene was detected by real-time PCR in 5 human nasopharyngeal carcinoma cell lines. The qualitative and quantitative expression levels of BRMS1 protein in 5 human nasopharyngeal carcinoma cell lines were detected by Western blot. Results 1. Real time fluorescence quantitative PCR method was used to detect, It was found that BRMS1 mRNAs were amplified from human nasopharyngeal carcinoma cells with different metastatic habits, and the expression level of BRMS1 mRNA decreased gradually with the increase of metastasis habit. The expression of BRMS1 protein in human nasopharyngeal carcinoma cells was detected by Western-blot technique. The results showed that the protein expression level of BRMS1 gene decreased gradually with the increase of cell metastasis habit. The expression of BRMS1 in nasopharyngeal carcinoma cells was detected by immunohistochemical method. The results showed that BRMS1 was expressed in the cell membrane and / or cytoplasm of nasopharyngeal carcinoma. Conclusion the expression of BRMS1 gene in different human nasopharyngeal carcinoma cell lines is consistent with the expression of mRNA and protein, and the expression of mRNA gene is consistent with the increase of mRNA level of BRMS1 gene. The expression of BRMS1 gene in mRNA decreased with the metastasis potential of nasopharyngeal carcinoma cell line. 3. BRMSI gene expression decreased with the increase of metastasis potential in nasopharyngeal carcinoma cell line at protein level. High expression in migration cell lines, However, the expression of BRMS1 in high metastatic cells decreased with the increase of metastatic potential, suggesting that BRMS1 may play an important role in the mechanism of metastasis of nasopharyngeal carcinoma.
【学位授予单位】：蚌埠医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R739.63

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