玻璃体腔注射VAS2870对小鼠氧诱导视网膜病变的保护作用

发布时间：2018-02-16 23:23

本文关键词： VAS 氧诱导视网膜病变氧自由基血管内皮生长因子　出处：《国际眼科杂志》2016年12期 　论文类型：期刊论文

【摘要】：目的:观察玻璃体腔注射VAS2870对C57小鼠氧诱导视网膜病变的影响。方法:将新生C57BL/6J小鼠随机分为3组,分别为正常对照组、VAS2870注射OIR组和无菌PBS缓冲液注射OIR组。将后两组小鼠在出生后第7d(P7)至P12置于体积分数为75%±2%的恒定高氧氧箱中以构建OIR模型,在P12时给予幼鼠双眼玻璃体腔注射VAS2870(0.5μL),另一组幼鼠双眼注射同等剂量的无菌PBS缓冲液。三组小鼠均在P17时取右眼行视网膜铺片和Lectin染色,观察视网膜中央无血管区及病理性新生血管的情况;取右眼行视网膜组织定量检测ROS/RNS含量;取左眼应用RT-PCR检测Nox4 m RNA含量,并应用Western-blot测定视网膜组织中VEGF的表达。结果:VAS2870注射OIR组小鼠视网膜中央无血管区面积较无菌PBS缓冲液注射OIR组明显减少(P0.05),病理性新生血管数目明显减少(P0.05);VAS2870注射OIR组小鼠视网膜组织Nox4 m RNA的表达量明显低于无菌PBS缓冲液注射组;VAS2870注射OIR组小鼠视网膜组织ROS含量较无菌PBS缓冲液注射组明显降低(P0.05);VAS2870注射OIR组小鼠视网膜组织VEGF的表达明显低于无菌PBS缓冲液注射组(P0.05)。结论:在小鼠OIR模型中,VAS2870可抑制Nox4 m RNA的表达,减少ROS/RNS,下调VEGF的生成,在视网膜病变进程中具有保护作用。
[Abstract]:Objective: to observe the effect of intravitreal injection of VAS2870 on oxygen-induced retinopathy in C57 mice. Methods: newborn C57BL / 6J mice were randomly divided into 3 groups. The mice in the latter two groups were placed in a constant hyperoxia box with volume fraction of 75% 卤2% on the 7th day after birth and in a constant hyperoxia box with a volume fraction of 75% 卤2% to establish the OIR model, and the control group were injected with VAS2870 and aseptic PBS buffer respectively, and the mice in the latter two groups were placed in a constant hyperoxic box with a volume fraction of 75% 卤2%. VAS2870(0.5 渭 L was injected into the vitreous body of the young rats at P12, and the same dose of aseptic PBS buffer was injected into the eyes of the other group. The right eyes of the three groups were taken out at P17 for retina preparation and Lectin staining. The contents of ROS/RNS in the retinal tissue of the right eye and the content of Nox4 m RNA in the left eye were measured by RT-PCR. Western-blot was used to detect the expression of VEGF in retina. Results the area of central retinal vascular area of mice injected with OIR was significantly lower than that of OIR group injected with sterile PBS buffer solution, and the number of pathological neovascularization was significantly decreased after injection of OIR with VAS2870. The expression of Nox4 m RNA in retina of mice in the control group was significantly lower than that in the control group injected with aseptic PBS buffer solution, and the content of ROS in the retina of the mice injected with OIR was significantly lower than that in the group injected with aseptic PBS buffer solution, and the VEGF in retina tissue of the OIR group was significantly lower than that of the group injected with aseptic PBS buffer. Conclusion: VAS2870 can inhibit the expression of Nox4 m RNA in mouse OIR model. Reducing RNSs and down-regulating the production of VEGF has a protective effect in the process of retinopathy.
【作者单位】：西安交通大学第一附属医院眼科;中国陕西省榆林市第一医院眼科;
【分类号】：R774.1

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