茶多酚对紫外线诱导大鼠晶状体上皮细胞中Caveolin-1、MMP-2表达的影响

发布时间：2018-03-03 14:15

本文选题：茶多酚　切入点：小窝蛋白-1　出处：《辽宁医学院》2012年硕士论文　论文类型：学位论文

【摘要】：目的研究小窝蛋白-1(caveolin-1, CAV-1)和基质金属蛋白酶-2（matrixmetalloproteinase-2，MMP-2）在氧化损伤性白内障的晶状体上皮细胞（lensepithelial cells, LECs）中的表达，并探讨茶多酚(tea polyphenols，，TP)对晶状体上皮细胞中CAV-1和MMP-2表达的影响，为探索TP可能成为防治白内障的新药物提供实验基础和理论依据。方法采用离体晶状体培养技术，通过紫外线（ultra-violet，UV）照射氧化损伤法建立实验性白内障晶状体模型，设置空白对照组、紫外线照射组和实验组（即UV+TP组），实验组按茶多酚的浓度分为4个亚组，即0.02mg/L、0.2mg/L、2mg/L、20mg/L，分别于6h、12h、24h、48h取样，进行形态学观察，运用免疫组织化学法染色计数晶状体上皮细胞中CAV-1、MMP-2阳性细胞，并运用Western-blot免疫印迹分析晶状体上皮细胞中CAV-1、MMP-2表达量的变化。结果空白对照组大鼠离体晶状体在培养的48h内无显著变化，晶状体均保持透明,紫外线照射组大鼠离体晶状体在培养的48h内,随紫外线作用时间的延长,晶状体的混浊程度逐渐加重。不同浓度TP实验组晶状体混浊进展较UV组缓慢，在不同时间段的混浊程度均明显低于UV组。不同时段各组晶状体上皮细胞Caveolin-1、MMP-2阳性表达率和表达量的比较,各实验组与紫外线对照组相比,差异有均有统计学意义(P0.05)，不同时段实验组晶状体上皮细胞CAV-1阳性表达明显高于UV组，MMP-2阳性表达明显低于UV组，0.02mg/L TP实验组CAV-1与空白对照组相比,差异无统计学意义(P0.05)；其余实验组与空白对照组相比，差异均有统计学意义(P0.05)。结论 CAV-1存在于正常晶状体上皮细胞中，在白内障晶状体上皮细胞中，随着紫外线照射时间的延长，CAV-1表达逐渐减少； MMP-2在正常晶状体上皮细胞中无表达，在白内障晶状体上皮细胞中，随着紫外线照射时间的延长，MMP-2的表达逐渐增加。TP可以抑制紫外线诱导的晶状体上皮细胞CAV-1表达的减少和MMP-2的增加，以浓度为0.02mg/L TP的作用效果最佳，提示TP有可能成为防治白内障的有效药物。
[Abstract]:Purpose. To investigate the expression of fossa protein 1 (CAV-1) and matrix metalloproteinase-2 (MMP-2) in lens epithelial cells (LECs) of oxidative cataract, and to investigate the effect of tea polyphenols tea polyphenols (TP) on the expression of CAV-1 and MMP-2 in lens epithelial cells. In order to explore TP may be a new drug for cataract prevention and treatment of experimental basis and theoretical basis. Method. The experimental cataract lens model was established by ultraviolet ultra-violetine (UV) irradiation and oxidative injury in vitro, and a blank control group was set up. Ultraviolet irradiation group and experimental group (UV TP group) were divided into four subgroups according to the concentration of tea polyphenols. The experimental group was divided into 4 subgroups according to the concentration of tea polyphenols, namely 0.02mg / L 0.2mg / L 0.2mg / L ~ (2) mg / L ~ (2) mg / L ~ (2) mg / L ~ (20) mg 路L ~ (-1) respectively at 6h ~ 12h ~ (24) h ~ (24 h) for morphological observation, and immunohistochemical staining was used to count the CAV-1MMP ~ (2) positive cells in lens epithelial cells. The expression of CAV-1 and MMP-2 in lens epithelial cells was analyzed by Western-blot Western blot. Results. In the blank control group, there was no significant change in the isolated lens within 48 hours of culture, and the lens remained transparent, while in the ultraviolet radiation group, the lens of the isolated lens cultured within 48 hours was prolonged with the time of ultraviolet irradiation. The degree of lens opacity was gradually aggravated. The progress of lens opacity in TP groups with different concentrations was slower than that in UV group. The positive rate and quantity of Caveolin-1 MMP-2 in lens epithelial cells in different periods of time were significantly lower than those in UV group, and the positive rate and quantity of MMP-2 in each experimental group were higher than those in ultraviolet control group. The positive expression of CAV-1 in lens epithelial cells in the experimental group was significantly higher than that in the UV group (P 0.05). The positive expression of MMP-2 in the experimental group was significantly lower than that in the UV group (0.02 mg / L TP) compared with the blank control group. There was no significant difference between the two groups (P 0.05), but there was significant difference between the other experimental groups and the blank control group (P 0.05). Conclusion. The expression of CAV-1 in normal lens epithelial cells decreased gradually with the prolongation of ultraviolet irradiation time, but MMP-2 was not expressed in normal lens epithelial cells, but in cataract lens epithelial cells. With the prolongation of ultraviolet irradiation time, the expression of MMP-2 increased gradually. TP could inhibit the decrease of CAV-1 expression and the increase of MMP-2 in lens epithelial cells induced by ultraviolet radiation. The best effect was 0.02mg / L TP. The results suggest that TP may be an effective drug for the prevention and treatment of cataract.
【学位授予单位】：辽宁医学院
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R776.1

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