重楼皂苷Ⅰ对低氧喉癌Hep-2细胞增殖和HIF-1α、VEGF表达的影响

发布时间：2018-03-04 14:19

本文选题：重楼皂苷Ⅰ　切入点：Hep-细胞　出处：《安徽医科大学学报》2016年11期 　论文类型：期刊论文

【摘要】：目的研究重楼皂苷Ⅰ对低氧喉癌Hep-2细胞增殖及缺氧诱导因子-1α(HIF-1α)、血管内皮生长因子(VEGF)表达的影响。方法采用MTT法检测重楼皂苷Ⅰ对细胞增殖的影响,实时定量PCR(RT-PCR)法检测低氧条件下重楼皂苷Ⅰ对Hep-2细胞HIF-1αmRNA、VEGF mRNA表达的影响。采用Western blot法检测低氧条件下,重楼皂苷Ⅰ对Hep-2细胞VEGF、HIF-1α、信号传导与转录激活因子3(STAT-3),磷酸化信号传导与转录激活因子3(p-STAT-3)蛋白表达的影响。结果重楼皂苷Ⅰ对低氧喉癌Hep-2细胞的增殖有抑制作用;其中,1.5、3、6μg/ml浓度组重楼皂苷Ⅰ可下调低氧Hep-2细胞HIF-1α、VEGF mRNA和蛋白的表达。同时,重楼皂苷Ⅰ抑制了STAT-3、p-STAT-3的表达。结论重楼皂苷Ⅰ能抑制低氧条件下喉癌Hep-2细胞增殖和HIF-1α、VEGF的表达,可能与其下调STAT-3表达及抑制STAT-3的磷酸化有关。
[Abstract]:Objective to study the effects of saponins I on the proliferation of Hep-2 cells and the expression of hypoxia inducible factor-1 伪 (HIF-1 伪) and vascular endothelial growth factor (VEGF) in hypoxic laryngeal carcinoma. The effect of saponins I on the expression of HIF-1 伪 mRNA-VEGF mRNA in Hep-2 cells was detected by real-time quantitative PCR- RT-PCR.The Western blot assay was used to detect the expression of HIF-1 伪 -mRNA-VEGF mRNA in Hep-2 cells under hypoxia. The expression of HIF-1 伪, signal transduction and transcriptional activator 3- STAT-3, phosphorylated signal transduction and transcriptional activator 3p-STAT-3) in Hep-2 cells were inhibited. Results the proliferation of Hep-2 cells in hypoxic laryngocarcinoma was inhibited by the expression of SAS-1 伪, phosphorylated signal transduction and transcriptional activator 3p-STAT-3. The expression of HIF-1 伪 -VEGF mRNA and protein in hypoxic Hep-2 cells was down-regulated in 1. 5 渭 g / ml and 6 渭 g / ml groups. At the same time, it inhibited the expression of STAT-3 and p-STAT-3.Conclusion it can inhibit the proliferation of Hep-2 cells and the expression of HIF-1 伪 VEGF in laryngeal carcinoma under hypoxia. It may be related to its down-regulation of STAT-3 expression and inhibition of STAT-3 phosphorylation.
【作者单位】：贺州市人民医院耳鼻喉头颈外科;湖北中医药大学临床医学院;
【基金】：湖北省卫生计生委中医药、中西医结合科研指导性项目(编号:2013Z-B05)
【分类号】：R739.65

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