LMP1经TRADD促进鼻咽癌SP18细胞增殖

发布时间：2018-03-05 16:18

本文选题：鼻咽癌　切入点：潜伏性膜蛋白　出处：《中国病理生理杂志》2017年04期 　论文类型：期刊论文

【摘要】：目的:探讨潜伏性膜蛋白1(LMP1)羧基末端活化区2(CTAR2)的肿瘤坏死因子受体相关死亡结构域(TRADD)在鼻咽癌SP18细胞增殖中的作用及机制。方法:首先建立表达LMP1及CTAR2突变型LMP1(LMP1~(TRADD))的SP18细胞系。其次采用细胞生长曲线、平皿集落形成实验、软琼脂集落实验和流式细胞术观察LMP1~(TRADD)对SP18细胞增殖的影响。然后选用基因芯片检测SP18-LMP1和SP18-LMP1~(TRADD)细胞间的差异表达基因。结果:细胞生长曲线、软琼脂集落和平皿集落形成实验结果均显示,SP18-LMP1细胞生长与集落形成能力均较SP18-LMP1~(TRADD)细胞强(P0.01)。流式细胞术检测结果显示,SP18-LMP1细胞的增殖指数较SP18-LMP1~(TRADD)细胞高(P0.01)。筛选出63个增殖相关基因,其中SP18-LMP1~(TRADD)细胞中上调的基因33个,下调的基因30个。结论:TRADD活性区是LMP1促进SP18细胞增殖的重要功能活性位点。LMP1可能通过TRADD提高细胞增殖指数,诱导SP18细胞增殖。
[Abstract]:Objective: to investigate the role and mechanism of tumor necrosis factor receptor-associated death domain (TRADDD) in the proliferation of nasopharyngeal carcinoma (NPC) SP18 cells in the carboxyl terminal activation region of latent membrane protein 1 (LMP1). Methods: to establish the expression of LMP1 and CTAR2 mutant LMP1 / TRADDD) in nasopharyngeal carcinoma (NPC) cells. Then the cell growth curve was used. The effects of LMP1 and TRADDD on the proliferation of SP18 cells were observed by plate colony forming assay, soft Agar colony assay and flow cytometry. The differentially expressed genes between SP18-LMP1 and SP18-LMP1 TRADDD cells were detected by gene microarray. The results showed that the growth and colony forming ability of SP18-LMP1 cells was stronger than that of SP18-LMP1 / TRADDD cells, and the proliferation index of SP18-LMP1 cells was higher than that of SP18-LMP1 / TRADDD cells by flow cytometry. 63 proliferation-related genes were screened out, and the results of flow cytometry showed that the proliferation index of SP18-LMP1 cells was higher than that of SP18-LMP1 / TRADDD cells, and the proliferation index of SP18-LMP1 cells was higher than that of SP18-LMP1 / TRADDD cells. Among them, 33 genes were up-regulated and 30 genes were down-regulated in SP18-LMP1 / TRADDD cells. Conclusion the active region of LMP1 is an important functional active site of LMP1 to promote the proliferation of SP18 cells. LMP1 may increase the cell proliferation index and induce the proliferation of SP18 cells through TRADD.
【作者单位】：南华大学附属第二医院耳鼻喉科;南华大学医学院肿瘤研究所肿瘤细胞与分子病理学湖南省重点实验室;南华大学医学院;
【基金】：湖南省自然科学衡阳联合基金资助项目(No.12JJ9033) 湖南省自然科学基金资助项目(No.13JJ3079) 湖南省科技厅项目(No.2013SK3118;No.2014SK3081) 湖南省高校创新平台基金资助项目(No.10K052;No.12K094;No.13K083) 湖南省教育厅课题(No.11C1112;No.12C0340) 湖南省卫生厅项目(No.B2014-163;No.B2013-048)
【分类号】：R739.63

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