miR-106b对鼻咽癌细胞凋亡的促进和对增殖的抑制作用

发布时间：2018-03-07 14:28

本文选题：miR-b　切入点：鼻咽癌　出处：《西安交通大学学报(医学版)》2017年06期 　论文类型：期刊论文

【摘要】：目的探讨miR-106b对鼻咽癌细胞凋亡和增殖的影响。方法应用miRNA芯片分析获得鼻咽癌组织和癌旁正常组织表达差异的miRNA,TaqMan miRNA检测试剂盒和实时荧光定量PCR分别检测miR-106和RhoC mRNA在鼻咽癌和癌旁组织中的表达,用miRnada预测miR-106b和靶基因的结合位点,采用双荧光素酶验证靶基因,Western blot检测miR-106b调控靶基因RhoC的表达水平。用Annexin V-PI和TUNEL检测miR-106b对鼻咽癌细胞凋亡的影响,用MTT检测miR-106b对鼻咽癌细胞增殖的影响。结果 miRNA芯片分析发现鼻咽癌组织中miR-106b的表达较癌旁正常组织低。RT-PCR结果显示miR-106b在鼻咽癌组织中表达减少(P0.05),RhoC在鼻咽癌组织中表达增加(P0.05),miR-106b和RhoC在鼻咽癌组织中表达呈负相关(r= 0.586 6,P0.001)。荧光素酶报告基因实验结果显示miR-106b组荧光素酶活性低于空质粒组(P0.05),Western blot结果显示miR-106b组鼻咽癌细胞中RhoC的表达较空质粒组降低(P0.05)。Annexin V-PI和TUNEL结果显示,miR-106b组鼻咽癌细胞凋亡较空质粒组增加(P0.05);MTT结果显示miR-106b组鼻咽癌细胞增殖能力较空质粒组降低(P0.05)。结论 miR-106b可能通过下调RhoC的表达诱导鼻咽癌细胞凋亡并抑制鼻咽癌细胞增殖。
[Abstract]:Objective to investigate the effect of miR-106b on apoptosis and proliferation of nasopharyngeal carcinoma cells. Methods the differential expression of miRNA-TaqMan miRNA assay kit and real-time fluorescent quantitative PCR were obtained by miRNA microarray analysis for miR-106 and RhoC in nasopharyngeal carcinoma tissues and adjacent normal tissues, respectively. Expression of mRNA in nasopharyngeal carcinoma and adjacent tissues. The binding sites of miR-106b and target gene were predicted by miRnada, the expression level of miR-106b regulated target gene RhoC was detected by double luciferase Western blot, and the effect of miR-106b on apoptosis of nasopharyngeal carcinoma cells was detected by Annexin V-PI and TUNEL. Results miRNA microarray analysis showed that the expression of miR-106b in nasopharyngeal carcinoma tissues was lower than that in adjacent normal tissues. RT-PCR results showed that the expression of miR-106b in nasopharyngeal carcinoma tissues was lower than that in nasopharyngeal carcinoma tissues. The results of luciferase reporter gene experiment showed that luciferase activity in miR-106b group was lower than that in empty plasmid group. Western blot showed that RhoC expression in nasopharyngeal carcinoma cells in miR-106b group was higher than that in miR-106b group. The results showed that the apoptosis of nasopharyngeal carcinoma cells in the empty plasmid group was higher than that in the empty plasmid group. The results showed that the proliferation ability of nasopharyngeal carcinoma cells in the miR-106b group was lower than that in the empty plasmid group. Conclusion miR-106b may induce the apoptosis of nasopharyngeal carcinoma cells by down-regulating the expression of RhoC. Apoptosis of nasopharyngeal carcinoma cells and inhibition of proliferation of nasopharyngeal carcinoma cells.
【作者单位】：川北医学院附属医院耳鼻咽喉头颈外科;川北医学院人体解剖教研室;大连医科大学第一临床学院;
【基金】：四川省科技厅重点项目资助(No.14ZA0198)~~
【分类号】：R739.63

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