ECRG4增强鼻咽癌细胞对顺铂敏感性的初步研究

发布时间：2018-03-09 08:04

本文选题：食管癌相关基因　切入点：顺铂　出处：《现代肿瘤医学》2016年05期 　论文类型：期刊论文

【摘要】：目的:研究ECRG4在鼻咽癌细胞对顺铂反应性中的影响。方法:以不同浓度的顺铂分别处理ECRG4稳定转染的CNE1细胞和对照细胞,MTT实验检测ECRG4高表达对鼻咽癌细胞对化疗敏感性的影响。流式细胞术、免疫荧光和Western Blot检测ECRG4高表达对顺铂处理后的鼻咽癌细胞的细胞凋亡和细胞自噬的影响。用抑制细胞自噬的药物预先处理鼻咽癌细胞,观察该药物对ECRG4高表达增强的鼻咽癌细胞化疗敏感性的影响。结果:和对照细胞相比,ECRG4的高表达显著增强鼻咽癌细胞对顺铂的敏感性。流式细胞术分析和细胞凋亡相关蛋白的Western Blot分析显示ECRG4高表达对顺铂诱导的鼻咽癌细胞的细胞凋亡没有显著影响。免疫荧光和Western Blot分析显示ECRG4的高表达上调自噬微管相关轻链蛋白3(LC3)的含量。同时抑制细胞自噬的药物可以降低ECRG4高表达对CNE1细胞对顺铂敏感性的促进作用。结论:ECRG4高表达促进鼻咽癌细胞对顺铂的敏感性,并且该作用是通过诱导细胞自噬的途径实现的。ECRG4有可能用于鼻咽癌个体化治疗方案的分子标记。
[Abstract]:Objective: to study the effect of ECRG4 on the reactivity of nasopharyngeal carcinoma cells to cisplatin. Methods: ECRG4 stably transfected CNE1 cells and control cells were treated with different concentrations of cisplatin respectively. Effects of sensitivity. Flow cytometry, The effects of high expression of ECRG4 on apoptosis and autophagy of nasopharyngeal carcinoma cells treated with cisplatin were detected by immunofluorescence and Western Blot. To observe the effect of the drug on chemosensitivity of nasopharyngeal carcinoma cells with high expression of ECRG4. Results: the high expression of ECRG4 G4 significantly enhanced the sensitivity of nasopharyngeal carcinoma cells to cisplatin. Flow cytometry and cell regulation. Western Blot analysis of apoptosis related proteins showed that the high expression of ECRG4 had no significant effect on apoptosis of nasopharyngeal carcinoma cells induced by cisplatin. Immunofluorescence and Western Blot analysis showed that ECRG4 overexpression upregulated autophagy associated light chain protein 3LC3). At the same time, inhibition of autophagy can reduce the effect of ECRG4 overexpression on the cisplatin sensitivity of CNE1 cells. Conclusion the high expression of ECRG4 RG4 can promote the sensitivity of nasopharyngeal carcinoma cells to cisplatin. This effect is achieved by inducing autophagy. ECRG4 may be used as a molecular marker for individualized treatment of nasopharyngeal carcinoma.
【作者单位】：湖北文理学院医学院;漯河医学高等专科学校药学系;
【基金】：湖北省自然科学基金项目(编号:2014CFC1154) 湖北文理学院医学院基金项目(编号:YXKY 201402)
【分类号】：R739.63

【相似文献】