局部注射鼠神经生长因子对眼外肌的作用研究

发布时间：2018-03-09 22:29

本文选题：眼外肌　切入点：鼠神经生长因子　出处：《广西医科大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的探讨兔眼外肌局部注射鼠神经生长因子(nerve growth factor, NGF)后的肌肉收缩能力及组织学变化,探索药物治疗斜视的新方法。方法以16只成年新西兰大白兔为实验对象,左眼上直肌注射0.05mL(5μg) NGF(实验组),右眼上直肌注射0.05mL生理盐水(对照组)。1周后将实验动物麻醉测量上直肌肌肉收缩力,测量完毕处死动物后获取上直肌,测量肌肉湿重,长度,计算肌肉横截面积,做石蜡切片并行形态学检查。结果(1)实验组眼外肌在单刺激及不同频率刺激下,肌力较对照组均明显增强,差异均有统计学意义(P0.05)。实验组上直肌抗疲劳性实验结果(166.00±12.46)s较对照组(150.30±14.49)s差异有统计学意义(P)0.05)。(2)两组肌肉湿重未见明显不同,实验组眼外肌肌肉横截面面积为(9.67±1.82)mm2,对照组眼外肌肌肉横截面面积为(6.97±1.90)mm2,实验组较对照组增大明显,差异有统计学意义(P0.05)。注射部位无明显炎性细胞浸润,未见眼外肌肌纤维变性、坏死等毒性反应。注射部位附近的巩膜和相应的色素膜也未见明显炎性变化。Van Gieson氏苦味酸酸性复红染色未见胶原异常增生。实验组眼外肌纤维周径为(167.20±35.55)μm,较对照组(127.44±33.64)μ m更大(P0.01)。实验组眼外肌纤维大细胞计数为136、中等细胞计数为381、小细胞计数为28,对照组分别为88、383、118,实验组大细胞和中等细胞比例较对照组增高,小细胞比例降低,差异有统计学意义(P0.01)。(3)实验组PCNA阳性细胞百分率为(21.93±5.08)%较对照组(12.97±2.68)%更大,差异有统计学意义(P0.01)。实验组PCNA(?)日性细胞总数占总细胞数的21.8%(664／3078),对照组为12.1%(347／2869),差异有统计学意义(P0.01)。结论眼外肌局部注射5μg鼠神经生长因子对眼部组织无明显不良作用。眼外肌局部注射鼠神经生长因子能短期迅速提高肌肉收缩能力。鼠神经生长因子能促使眼外肌肌纤维快速增生,并能促进组织细胞分化。
[Abstract]:Objective to investigate the muscle contractility and histological changes after local injection of nerve growth factor nerve growth factor (NGF) into rabbit extraocular muscles, and to explore a new method for the treatment of strabismus. Methods Sixteen adult New Zealand white rabbits were used to measure the contractility of the superior rectus muscle by injecting 0.05mLL of 5 渭 g of NGF into the left superior rectus muscle (experimental group) and 0.05 mL saline (the control group was injected with 0.05 mL normal saline) after 1 week of anaesthesia, and the contractility of the muscle of the superior rectus muscle was measured. After the animals were killed, the superior rectus muscle was obtained, the wet weight and length of the muscle were measured, the cross sectional area of the muscle was calculated, the paraffin section was made and the morphological examination was performed. Results 1) the muscle strength of the extraocular muscle in the experimental group was significantly higher than that in the control group under single stimulation and different frequency stimulation. There was no significant difference in muscle wet weight between the experimental group and the control group (166.00 卤12.46 vs 150.30 卤14.49 vs 150.30 卤14.49). The cross sectional area of the extraocular muscle in the experimental group was 9.67 卤1.82 mm ~ (-2), and that in the control group was 6.97 卤1.90 mm ~ (2), which was significantly larger than that in the control group (P < 0.05). There was no significant infiltration of inflammatory cells and no degeneration of the extraocular muscle at the injection site. No inflammatory changes were observed in the sclera near the injection site and the corresponding pigment membrane. There was no abnormal proliferation of collagen by van Gieson picric acid acid fuchsin staining. The peripheral diameter of the extraocular muscle fibers in the experimental group was 167.20 卤35.55 渭 m, which was higher than that in the control group. In the experimental group, the number of fibrous large cells in extraocular muscle was 136, the number of medium cells was 381, the number of small cells was 28, and the number of small cells in the control group was 88-383118.The ratio of large cells and medium cells in the experimental group was higher than that in the control group. The percentage of PCNA positive cells in the experimental group was 21.93 卤5.08%, which was higher than that in the control group (12.97 卤2.68%). ) the total number of diurnal cells accounted for 21.8% of the total number of cells, and that of the control group was 12.1%, 347 / 2869% (P 0.01). Conclusion Local injection of 5 渭 g nerve growth factor into the extraocular muscle has no obvious adverse effect on ocular tissue. Local injection of nerve growth factor into the extraocular muscle can improve the contractility of the muscle in a short period of time, and nerve growth factor can promote the eye. The muscle fibers of the external muscles proliferate rapidly. And can promote tissue and cell differentiation.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R777.4

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