CXCL1在大鼠烟曲霉菌角膜炎早期的表达

发布时间：2018-03-10 08:34

本文选题：CXCL1　切入点：真菌性角膜炎　出处：《青岛大学》2012年硕士论文　论文类型：学位论文

【摘要】：目的观察CXCL1在大鼠烟曲霉菌角膜炎早期角膜组织的表达情况,探讨其在真菌性角膜炎固有免疫阶段的作用。方法健康Wistar大鼠66只,雌雄不限,随机分为3组,正常对照组6只12眼,损伤对照组30只30眼,真菌感染组30只30眼,损伤对照组和真菌感染组动物均取右眼为实验眼。刮除角膜中央上皮后涂以烟曲霉菌菌丝悬液并覆盖自制角膜接触镜,建立角膜烟曲霉菌感染模型。建模后分别于4,8,16,24h处死大鼠,HE染色观察角膜组织病理变化和中性粒细胞浸润,运用RT-PCR和免疫组织化学技术检测各组不同时间点CXCL1的表达情况。结果建模后裂隙灯检查见大鼠角膜化脓性溃疡表现,刮取物真菌培养有烟曲霉菌菌落生长。HE染色见真菌感染组角膜炎症改变,大量中性粒细胞浸润。RT-PCR示接种真菌后4h角膜即出现CXCL1表达,并在24h内持续升高(P=0.000)。CXCL1在正常对照组和模型组角膜上皮各检测时间点的表达量有显著差异(各组比较P=0.000)。免疫组化结果示在角膜上皮,正常对照组有少量的CXCL1表达,损伤对照组表达升高,与正常对照组比较差异有统计学意义(P=0.000)；真菌感染组CXCL1表达较损伤对照组更高,与正常对照组和损伤对照组比较差异有统计学意义(P=0.000)。结论1、组织病理学观察和真菌培养证实大鼠烟曲霉菌性角膜炎模型建立成功。2、大鼠烟曲霉菌性角膜炎早期主要特点为大量中性粒细胞浸润。3、CXCL1基因转录和蛋白表达在烟曲霉菌角膜炎早期均显著升高,提示CXCL1可能与中性粒细胞的浸润相关。
[Abstract]:Objective to investigate the expression of CXCL1 in the early corneal tissue of rat aspergillus fumigatus keratitis and its role in the innate immune stage of fungal keratitis. Methods 66 healthy Wistar rats were randomly divided into 3 groups: normal control group (n = 6), injury control group (n = 30), fungal infection group (n = 30), normal control group (n = 6), injury control group (n = 30) and fungal infection group (n = 30). The right eyes of the injured control group and fungal infection group were taken as experimental eyes. The central epithelium of cornea was scraped with aspergillus fumigatus hyphae suspension and covered with self-made contact lens. The model of aspergillus fumigatus infection was established and the rats were killed at 4: 8 and 1624 h to observe the corneal histopathological changes and neutrophil infiltration. The expression of CXCL1 at different time points was detected by RT-PCR and immunohistochemistry. Results the corneal suppurative ulcer was detected by slit lamp in rats after modeling. The corneal inflammation was observed in the fungal culture group with aspergillus fumigatus colony growth and HE staining. A large number of neutrophils infiltrated. RT-PCR showed that there was CXCL1 expression in cornea 4 hours after inoculation. The expression of CXCL1 in corneal epithelium of normal control group and model group was significantly higher than that of control group in 24 h. The immunohistochemical results showed that there was a small amount of CXCL1 expression in corneal epithelium in normal control group and normal control group, and the expression of CXCL1 in normal control group was a little higher than that in normal control group. Compared with the normal control group, the expression of CXCL1 in the fungal infection group was higher than that in the injured control group, and the difference was statistically significant compared with the normal control group and the injured control group. Conclusion 1. Histopathological observation and fungal culture confirmed that the rat model of tobacco-curl fungal keratitis was established successfully. The main characteristics of rat early keratitis were neutrophil infiltration. 3CXCL1 gene transcription and protein surface. In the early stage of aspergillus fumigatus keratitis, The results suggest that CXCL1 may be associated with neutrophil infiltration.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R772.21

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