siRNA沉默Survivin基因对鼻咽癌CNE-2细胞凋亡的影响

发布时间：2018-03-12 23:13

本文选题：鼻咽癌　切入点：RNA干扰　出处：《基因组学与应用生物学》2017年05期 　论文类型：期刊论文

【摘要】：合成Survivin小干扰RNA(small interfering RNA,siRNA)序列,用于转染人鼻咽癌细胞株CNE2,体外观察Survivin siRNA转染后CNE-2细胞Survivin mRNA、蛋白的表达、细胞增殖和凋亡情况。设计合成3段特异性Survivin siRNA,用siRNA转染鼻咽癌CNE-2细胞株,设置空白对照和阴性对照组,通过Real-time PCR检测CNE-2细胞的Survivin mRNA相对表达量,Western blotting检测Survivin蛋白的表达,流式细胞术及原位细胞凋亡检测转染后细胞凋亡情况。siRNA转染CNE-2细胞后,siRNA 1组和3组mRNA表达抑制率分别为(68.46±7.94)%、(49.44±3.78)%,siRNA 2组结果无显著差异(p0.05)。蛋白相对表达量只有siRNA 1组降低,表达抑制率为(30.61±2.47)%,流式细胞术和原位细胞凋亡检测转染后CNE-2细胞数量明显降低,细胞凋亡比例增高。siRNA干扰沉默Survivin基因能有效抑制CNE-2细胞的增殖和诱导细胞凋亡。本研究为Survivin基因可能作为治疗鼻咽癌的一个靶点,为Survivin基因沉默可能是治疗鼻咽癌高效的途径提供体外实验支持。
[Abstract]:Synthesis of Survivin small interfering RNA (siRNA small interfering RNA) sequence for transfection of human nasopharyngeal carcinoma cell line CNE2, observe the Survivin siRNA transfected CNE-2 cells after Survivin mRNA, protein expression, cell proliferation and apoptosis. The design and synthesis of 3 specific Survivin siRNA, transfected with siRNA nasopharyngeal carcinoma cell line CNE-2, and blank control the negative control group, detected by CNE-2 cell Real-time PCR Survivin mRNA relative expression, detect the expression of Survivin protein Western blotting, flow cytometry and TUNEL positive cells after transfection the apoptosis of.SiRNA after transfection into CNE-2 cells, the expression of siRNA 1 group and 3 group mRNA inhibition rate were (68.46 + 7.94)%. (49.44 + 3.78)%, the 2 groups showed no significant difference in siRNA (P0.05). The relative expression of siRNA protein only 1 groups decreased, expression inhibition rate was (30.61 + 2.47)%, flow cytometry and in situ fine The number of CNE-2 cells after transfection was detected by cell apoptosis significantly decreased, apoptosis increased ratio of.SiRNA interference silencing Survivin gene can effectively inhibit the proliferation of CNE-2 cells and induce cell apoptosis. In this study, Survivin gene may serve as a target for the treatment of nasopharyngeal carcinoma, Survivin gene silencing may be effective way for the treatment of nasopharyngeal carcinoma in vitro to provide support.

【作者单位】：海南大学农学院;海南省人民医院医学检验中心海南省细胞与分子遗传转化医学重点实验室;
【基金】：海南省自然科学基金项目(20158359)资助
【分类号】：R739.63

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