转基因荧光小鼠视神经轴索钝性损伤后退行性病变观察

发布时间：2018-03-16 05:38

本文选题：GFP小鼠　切入点：YFP小鼠　出处：《眼科新进展》2017年08期 　论文类型：期刊论文

【摘要】：目的建立单侧眼视神经轴索钝性损伤模型,观察视神经轴突退行性病变过程和小胶质细胞的变化。方法将标记神经轴突的YFP小鼠和标记小胶质细胞的GFP小鼠分为手术组和正常对照组,并于视神经轴索钝性损伤手术后4 h、1 d、3 d、5 d、10 d分离视神经,以激光共聚焦显微镜观察神经轴突受损程度及小胶质细胞的变化。结果与对正常对照组比较,YFP小鼠术后4 h损伤处视神经轴突断裂;术后1 d视神经轴突开始出现念珠化;术后3 d视神经轴突大部分念珠化;术后5 d视神经轴突开始从念珠状转变为碎片状;术后10 d视神经轴突形成大量碎片。GFP小鼠与正常对照组相比,术后4 h形成胶质瘢痕,静息态小胶质细胞开始大量出现;术后1 d激活态小胶质细胞大量增多并开始覆盖受损区域;术后3 d大量的激活态小胶质细胞基本覆盖了受损区域;术后5 d、10 d虽然视神经的退行性病变持续恶化,但是小胶质细胞的数量基本保持稳定。结论小鼠视神经受损后轴突发生不可逆的退行性病变,同时并伴随着小胶质细胞的激活和增多,说明小胶质细胞与视神经的退行性病变密切关联。
[Abstract]:Objective to establish a model of unilateral optic nerve axonal blunt injury. To observe the process of optic nerve axonal degeneration and the changes of microglia. Methods YFP mice labeled with nerve axons and GFP mice labeled with microglia were divided into operation group and normal control group. The optic nerve was separated from the optic nerve at 4 h, 1 day, 3 days, 5 days and 10 days after the operation of blunt axonal injury of optic nerve. Results compared with the normal control group, the optic nerve axons of the YFP mice were damaged 4 hours after operation, and the axons of the optic nerve began to appear rosary on the 1st day after operation, and the damage degree of the axons and the changes of microglia were observed by laser confocal microscope. On the 3rd day after operation, most of the axons of the optic nerve became beads, 5 days after the operation, the axons of the optic nerve began to change from the beads to the fragments, and on the 10th day after the operation, a large number of fragments were formed in the optic nerve axons. Compared with the normal control group, the glial scar was formed in the GFP mice at 4 hours after operation. Resting microglia began to appear in large numbers, activated microglia increased a lot and began to cover the damaged area on the 1st day after operation, and a large number of activated microglia covered the damaged area on the 3rd day after operation. Although the degenerative lesion of optic nerve continued to deteriorate on the 5th day after operation, the number of microglia remained stable. Conclusion there is irreversible degeneration of the axon after optic nerve injury in mice. At the same time, the activation and increase of microglia showed that microglia were closely related to the degeneration of optic nerve.
【作者单位】：兰州大学;
【基金】：国家自然科学基金资助(编号:31471953)~~
【分类号】：R774.6

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