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孔源性视网膜脱离术后视网膜下液延迟吸收的相关研究

发布时间:2018-03-19 03:03

  本文选题:肾上腺素 切入点:视网膜脱离 出处:《吉林大学》2012年硕士论文 论文类型:学位论文


【摘要】:目的:探讨孔源性视网膜脱离(Rhegatogenous Retinal Detachment,RRD)巩膜扣带术后视网膜下液(Subretinal Fluid,SRF)的延迟吸收与血液中肾上腺素浓度的相关性,分析其原因,以期缩短视网膜脱离术后SRF的吸收时间。 方法:采用病例对照的方法,分析2009年-2011年吉林大学第二临床学院眼底病二科行巩膜扣带术(包括环扎+冷凝、环扎+外垫压+冷凝、外垫压+冷凝,放液或不放液)的孔源性视网膜脱离患者44例(45只眼),男性21例(22只眼),女性23例(23只眼),年龄18-70岁,平均41.38±14.17岁。44例患者均为新鲜的孔源性视网膜脱离患者,所有患者术前视网膜脱离的范围在1.9±0.8个象限。术后视网膜原裂孔封闭良好,未发现新裂孔,无明显的PVR,术后眼压稳定在正常范围内,视网膜下液在术后1天-6个月吸收。上述病例均未实施第二次手术,术后视网膜下液逐渐减少,随诊1年,视网膜复位良好。按术后视网膜下液吸收的时间分为A组(≤3天)、 B组(3天到1个月)和C组(>1个月)三组,其中B、C两组视为“视网膜下液延迟吸收”组。三组患者均在术前1天及术后第1天清晨空腹肘静脉采血,患者静坐30min后前臂静脉采血5mL,采集血液置于预冷肝素化的真空采血管中,样本收集后30min内在4度下离心3000r/min、30min后取上清,置-80度保存,待检。标本采用长春比尔生物科技有限公司提供的肾上腺素ELISA检测试剂盒,运用生物素双抗体夹心酶联免疫吸附法(ELISA)测定血清中的人肾上腺素(EPI)水平,,操作严格按试剂盒说明书进行。数据搜集完毕,SPSS16.0统计学软件进行数据分析,比较三组患者术前1天及术后第1天、术后不同性别组以及每组术前1天与术后第1天的肾上腺素水平。 结果:(1)术前1天三组间血清肾上腺素水平比较:A组与B组, P=0.0130.05,差异有统计学意义;A组与C组, p=0.0050.05,差异有统计学意义;B组与C组,p=0.362>0.05,差异无统计学意义。(2)术后第1天各组血清中肾上腺素值比较:A组与B组, p=0.0000.05,差异有统计学意义;A组与C组,p=0.000<0.05,差异有统计学意义;B组与C组, p>0.05,差异无统计学意义.(3)各组组内术前1天与术后第1天肾上腺素水平比较:A组,p=0.0390.05,差异有统计学意义;B组,p0.05,差异无统计学意义;C组,p0.05,差异无统计学意义。(4)B组、C组中男、女术后肾上腺素比较, p=0.478>0.05,差异无统计学意义。 结论:1.血清肾上腺素浓度与孔源性视网膜脱离术后视网膜下液的吸收具有相关性,肾上腺素水平高者视网膜下液吸收慢。2.特殊类型人群可根据术前血清肾上腺素浓度预测术后视网膜下液吸收的时间。
[Abstract]:Objective: to investigate the relationship between the delayed absorption of subretinal fluid (subretinal fluid) and the concentration of epinephrine in the blood after rhegmatogenous Retinal detachment (RRD) scleral buckling, and to analyze the causes, so as to shorten the time of SRF absorption after retinal detachment. Methods: from 2009 to 2011, scleral buckling was performed in the second Department of ophthalmopathy, Jilin University, from 2009 to 2011. There were 44 patients with rhegmatogenous retinal detachment (44 patients) with or without rhegmatogenous retinal detachment, including 21 males (22 eyes) and 23 females (23 eyes) aged 18-70 years (mean 41.38 卤14.17 years), all of whom were fresh rhegmatogenous retinal detachment patients. The range of retinal detachment before operation was 1.9 卤0.8 quadrant. After operation, the retinal original hole was well closed, no new hole was found, no significant PVR was found, and the intraocular pressure was stable in the normal range. The subretinal fluid was absorbed from 1 day to 6 months after operation. According to the time of subretinal fluid absorption, group A (鈮

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