鼻咽癌中Rta-IgG表达的研究

发布时间：2018-03-20 19:24

本文选题：鼻咽癌　切入点：EB病毒　出处：《昆明医科大学》2017年硕士论文　论文类型：学位论文

【摘要】：[研究背景]鼻咽癌(NPC)是一种常见的头颈部肿瘤。EB病毒(Epstein-Barr virus,EBV)感染与鼻咽癌密切相关,目前研究表明可以通过Rta蛋白抗体IgG(Rta-IgG)的水平对其进行检测。[目的]抽取未经治疗的鼻咽癌患者和未患此病的健康人的血样,使用ELISA研究血液中Rta蛋白抗体IgG(Rta-IgG)的表达,再与VCA-IgA和EA-IgA进行比较。本研究的目的是评估Rta-IgG在鼻咽癌患者体内的表达情况,明确其临床应用和诊断价值。[方法]在这项研究中,共收集了 61名鼻咽癌患者作为研究人群,选取同时期19例健康人作为对照组。使用酶联免疫吸附测定法(ELISA)检测实验人群血清水平的Rta-IgG,VCA-IgA和EA-IgA水平。通过使用金标准筛选试验来进一步评估这些指标的诊断价值,以找出有效性,可靠性和预测价值。[结果]Rta-IgG,VCA-IgA,EA-IgA这三个参数的表达均具有显着的差异具有统计学意义,Rta-IgG(P = 0.013),VCA-IgA(P0.001),EA-IgA(P =0.002)。在单次试验中,Rta-IgG和VCA-IgA表达之间的有效性、可靠性和预测价值差异不大;而在多次试验中(其中Rta-IgG,VCA-IgA,EA-Ig-A组合观察),Rta-IgG和VCA-IgA都比EA-IgA具有更高的有效性、可靠性和预测价值。平行试验显示出较高的灵敏度但是特异性较低,而序列试验则显示较高的特异性,较低的敏感性。据ROC曲线分析,总体诊断效能排名为:VCA-IgA,Rta-IgG 和 EA-IgA.[结论]Rta-IgG,VCA-IgA和EA-IgA在鼻咽癌患者血清中表达水平升高。联合使用Rta-IgG,VCA-IgA和EA-IgA这三种参数用于诊断鼻咽癌可能对癌症病人群体筛选更有临床意义。
[Abstract]:[background] Nasopharyngeal carcinoma (NPC) is a common head and neck tumor. Epstein-Barr virus (EBV) infection is closely related to nasopharyngeal carcinoma. Current studies have shown that Rta protein antibody Rta-IgG can be detected by its level. [objective] to study the expression of Rta protein antibody IgG-Rta-IgG in the blood of patients with untreated nasopharyngeal carcinoma (NPC) and healthy persons without the disease, using ELISA. The purpose of this study was to evaluate the expression of Rta-IgG in patients with nasopharyngeal carcinoma and to determine its clinical application and diagnostic value. [methods] in this study, 61 patients with nasopharyngeal carcinoma were collected as a study group. The serum levels of Rta-IgGV VCA-IgA and EA-IgA were determined by enzyme linked immunosorbent assay (Elisa) in 19 healthy people in the same period. The diagnostic value of these indexes was further evaluated by using gold standard screening test. In order to find out the validity, reliability and predictive value. [results] the expression of Rta-IgGN VCA-IgACA EA-IgA was significantly different. There was no significant difference in the validity, reliability and predictive value between Rta-IgG and VCA-IgA expression in a single trial. In many experiments (Rta-IgG VCA-IgAEA-Ig-A combination), Rta-IgG and VCA-IgA are more effective, reliable and predictive than EA-IgA. Parallel tests show higher sensitivity but lower specificity, while sequence tests show higher specificity. Lower sensitivity. According to ROC curve analysis, The overall diagnostic efficacy ranked as Rta-IgG and EA-IgA. [conclusion] the expression of Rta-IgGG VCA-IgA and EA-IgA in the serum of patients with nasopharyngeal carcinoma is increased. The combination of Rta-IgGG VCA-IgA and EA-IgA may have more clinical significance in the diagnosis of nasopharyngeal carcinoma.
【学位授予单位】：昆明医科大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R739.63

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