IL-19及其受体与慢性鼻-鼻窦炎组织重塑的相关性研究

发布时间：2018-03-30 11:36

本文选题：慢性鼻-鼻窦炎　切入点：组织重塑　出处：《中国病理生理杂志》2017年05期

【摘要】：目的:探讨白细胞介素19(interleukin-19,IL-19)及其受体(IL-20R1/IL-20R2)在不同类型慢性鼻-鼻窦炎[慢性鼻-鼻窦炎伴鼻息肉(chronic rhinosinusitis with nasal polyps,CRSw NP)和慢性鼻-鼻窦炎不伴鼻息肉(chronic rhinosinusitis without nasal polyps,CRSs NP)]患者中表达的差异,并分析其与慢性鼻-鼻窦炎组织重塑的相关性。方法:研究共分3组:CRSw NP组(30例)、CRSs NP组(15例)和对照组(鼻中隔偏曲患者15例)。实时荧光定量PCR检测IL-19及其受体(IL-20R1/IL-20R2)和组织重塑因子基质金属蛋白酶(MMP)-2、MMP-9、金属蛋白酶组织抑制物(TIMP)-1的mRNA相对表达量;免疫组化检测IL-19及其受体的蛋白表达量;ELISA检测MMP-9、TIMP-1的蛋白表达量。结果:CRSw NP组IL-19、IL-20R1、IL-20R2和MMP-9的mRNA和蛋白表达量高于CRSs NP组和对照组(P0.05),CRSs NP组TIMP-1的mRNA和蛋白的表达量均高于CRSw NP组和对照组(P0.05);MMP-2的mRNA和蛋白的表达量在各组无显著差异。CRSw NP组IL-19、IL-20R1和IL-20R2均与MMP-9的mRNA相对表达量呈正相关(P0.05);IL-19及其受体与TIMP-1的mRNA相对表达量无明显相关。结论:IL-19及其受体的mRNA在CRSw NP中高表达,且与MMP-9 mRNA表达呈正相关,提示二者可能存在交互作用,可能参与慢性鼻-鼻窦炎的组织重塑。
[Abstract]:Objective: to investigate the expression of interleukin-19 interleukin-19 (IL-19) and its receptor IL-20R1 / IL-20R2 in patients with chronic rhinosinusitis [chronic rhinosinusitis with chronic rhinosinusitis with nasal polyps] and chronic rhinosinusitis without chronic rhinosinusitis without nasal polyps (CRSNPs). Methods: the study was carried out in 30 cases of 30 cases of CRSs NP group and 15 cases of control group (15 cases of nasal septum deviation patients). Real-time fluorescence quantitative PCR was used to detect IL-19 and its receptor in patients with chronic rhinosinusitis and chronic rhinosinusitis. MRNA expression of IL-20R1 / IL-20R2), MMP-2MMP-2MMP-9, tissue inhibitor of metalloproteinase (TIMP- 1), tissue remodeling factor (MMP-2MMP-2MMP 9), tissue inhibitor of metalloproteinase (TIMP- 1); The protein expression of IL-19 and its receptor was detected by immunohistochemistry and the protein expression of MMP-9 and TIMP-1 was detected by Elisa. Results the mRNA and protein expression of IL-19, IL-20R1, IL-20R2 and MMP-9 in the group of IL-19 / CRSw NP was higher than that in the group of CRSs NP and control group (P 0.05) and the expression of TIMP-1 protein was higher than that in the group of CRSw. There was no significant difference in the expression of mRNA and protein between NP group and control group. There was no significant correlation between the expression of IL-19 IL-20R1 and IL-20R2 and the relative expression of mRNA of MMP-9 in CRSw NP group. Conclusion there is no significant correlation between the expression of IL-19 and its receptor and the relative expression of mRNA of TIMP-1 in the NP group and the control group. Conclusion\\\; Somatic mRNA was overexpressed in CRSw NP. There was a positive correlation with the expression of MMP-9 mRNA, which suggested that there might be interaction between the two proteins, which might be involved in the tissue remodeling of chronic rhinosinusitis.
【作者单位】：中山大学附属第三医院耳鼻咽喉头颈外科;
【基金】：国家自然科学基金资助项目(No.81371073;No.81670913) 广东省自然科学研究计划项目(No.2013B021800081)
【分类号】：R765

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